Optimized operation of a controlled stirred tank reactor system for the production of mesenchymal stromal cells and their extracellular vesicles

Fernandes‐Platzgummer, Ana; Cunha, Raquel; Morini, Sara; Carvalho, Marta; Moreno-Cid, Juan A.; Silva, Claudio Lobato da

doi:10.22541/au.167160787.74320022/v1

Cited by 1 publication

(1 citation statement)

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“…The stirred tank reactor (STR) is the most commonly used bioreactor for culturing immobilized cells. 198,199 Owing to their advantages such as easy scale-up, good fluid mixing and oxygen transfer ability, and alternative impellers, STRs are commonly used. However, this type of bioreactor also has several limitations, such as high power consumption, high shear, and concerns about sealing and stability of shafts in tall bioreactors.…”

Section: Stirred Tank Reactor (Str)mentioning

confidence: 99%

Immobilization: the promising technique to protect and increase the efficiency of microorganisms to remove contaminants

Najim,

Radeef,

al‐Doori

et al. 2024

J of Chemical Tech & Biotech

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Biodegradation is one of the most economical methods and usually no production of toxic by‐products. In general, this approach is favored because it offers reduced expenses and the potential for complete mineralization. In order to enhance the viability and longevity of the bioremediation agents within polluted areas, it becomes necessary to immobilize the cells. Cell immobilization refers to the procedure of confining intact cells to specific areas within a device or material, without compromising their essential biological functions. A wide variety of carriers and approaches have been used for the restriction of various cells. Immobilization techniques, such as microencapsulation, have opened up new possibilities in biotechnology by facilitating the development of artificial organs, cell therapies, and drug delivery systems. Researchers have found promising outcomes in various applications through the immobilization of microorganisms. This approach enhances stability, reusability, and catalytic efficiency, making immobilization a valuable strategy for biocatalysis, bioremediation, and other biotechnological processes. Notably, the use of immobilized microorganisms has led to significant improvements in the removal of pollutants, with some studies achieving 100% efficiency. When comparing the degradation of pollutants between free and immobilized microorganisms over the same time period, the results demonstrated that immobilized microorganisms achieved a removal efficiency of more than 21% than the free microbial consortium. The primary objective of this review is to give an overview of the key scientific aspects related to bioremediation of various pollutants using immobilized cell, with a particular focus on the techniques to entrap the cells.This article is protected by copyright. All rights reserved.

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Section: Stirred Tank Reactor (Str)mentioning

confidence: 99%