Optimized molecular design of PET probe for the visualization of <i>γ</i>-glutamyltranspeptidase activity in tumors

Wang, Xiuting; Gao, Dingyao; Lu, Chunmei; Xie, Minhao; Lin, Jianguo; Qiu, Ling

doi:10.1039/d2nj01688e

Cited by 3 publications

(3 citation statements)

References 38 publications

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Section: Measuring Ggt By a Small-molecule-based Responsive Fluoresce...mentioning

confidence: 99%

“…Measuring the activity of GGT has also been achieved by alternative techniques, including bioluminescence, chemiluminescence, photoacoustic imaging (PAI), Raman, , ionizing-radiation-based techniques, like positron emission tomography (PET), − and magnetic resonance imaging (MRI). , In comparison with the responsive probes used for fluorescence measurements, the availability of probes employing these techniques for GGT measurements is limited. In this section, advances in the development of probes using these alternative techniques are summarized.…”

Section: Measuring Ggt By a Small-molecule-based Responsive Fluoresce...mentioning

confidence: 99%

“…The [ 18 F]-based PET probe ([ 18 F]JM-10, Probe #42) was also developed by Wang et al in 2022 for GGT measurement (Figure ). Interestingly, the probe [ 18 F]JM-10 could respond to GGT on the cell membrane to produce [ 18 F]JM-C. After internalization of [ 18 F]JM-C, [ 18 F]JM-D could be produced that could then be self-assembled through p-stacking to form the nanoparticle with 179 ± 20 nm diameter. The probe [ 18 F]JM-10 was finally applied to measure GGT activity in tumors.…”

Section: Measuring Ggt By a Small-molecule-based Responsive Fluoresce...mentioning

confidence: 99%

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Advances and Perspectives of Responsive Probes for Measuring γ-Glutamyl Transpeptidase

Zhang,

et al. 2023

ACS Meas. Sci. Au

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Gamma-glutamyltransferase (GGT) is a plasma-membrane-bound enzyme that is involved in the γ-glutamyl cycle, like metabolism of glutathione (GSH). This enzyme plays an important role in protecting cells from oxidative stress, thus being tested as a key biomarker for several medical conditions, such as liver injury, carcinogenesis, and tumor progression. For measuring GGT activity, a number of bioanalytical methods have emerged, such as chromatography, colorimetric, electrochemical, and luminescence analyses. Among these approaches, probes that can specifically respond to GGT are contributing significantly to measuring its activity in vitro and in vivo. This review thus aims to highlight the recent advances in the development of responsive probes for GGT measurement and their practical applications. Responsive probes for fluorescence analysis, including "off−on", near-infrared (NIR), two-photon, and ratiometric fluorescence response probes, are initially summarized, followed by discussing the advances in the development of other probes, such as bioluminescence, chemiluminescence, photoacoustic, Raman, magnetic resonance imaging (MRI), and positron emission tomography (PET). The practical applications of the responsive probes in cancer diagnosis and treatment monitoring and GGT inhibitor screening are then highlighted. Based on this information, the advantages, challenges, and prospects of responsive probe technology for GGT measurement are analyzed.

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Section: Measuring Ggt By a Small-molecule-based Responsive Fluoresce...mentioning

confidence: 99%

Section: Measuring Ggt By a Small-molecule-based Responsive Fluoresce...mentioning

confidence: 99%

Section: Measuring Ggt By a Small-molecule-based Responsive Fluoresce...mentioning

confidence: 99%

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Advances and Perspectives of Responsive Probes for Measuring γ-Glutamyl Transpeptidase

Zhang,

et al. 2023

ACS Meas. Sci. Au

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A sensitive benzothiazole fluorescent probe for the detection of γ‐glutamyl transpeptidase activity and its application

Wu,

Ding,

Yang

et al. 2024

Luminescence

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A sensitive benzothiazole fluorescent probe (PBZO) for the detection of γ‐glutamyl transpeptidase (GGT) activity was developed. Based on the enzymatic hydrolysis of peptide bonds by glutamyl transpeptidase, it can be specifically recognized by PBZO. The PBZO has a good linear relationship with different gradients of GGT activity at the emission wavelength of 560 nm, the Stokes shift reached 215 nm, and the detection limit of GGT activity is 0.1644 U/ml. With the increase of GGT concentration in the probe solution, the color of the solution gradually changed from orange to dark yellow under the 365 nm UV lamp. The same color change was also observed on the probe test paper. In addition, there is a linear relationship between the GGT activity and the R‐value of the probe solution. More importantly, the probe has a good recovery rate in serum. Therefore, this probe can be used as a convenient tool for detecting GGT activity.

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Lysosome-targeting and legumain-triggered 68Ga-labeled probe for enhanced tumor PET imaging

Hua,

Xi,

Xie

et al. 2024

Biochemical and Biophysical Research Communications

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Optimized molecular design of PET probe for the visualization of γ-glutamyltranspeptidase activity in tumors

Abstract: γ-Glutamyltranspeptidase (GGT), a protease anchored to the surface of the cell membrane, is responsible for maintaining intracellular redox balance by regulating endogenous glutathione and cysteine levels. The abnormal expression of...

Cited by 3 publications

References 38 publications

Advances and Perspectives of Responsive Probes for Measuring γ-Glutamyl Transpeptidase

Advances and Perspectives of Responsive Probes for Measuring γ-Glutamyl Transpeptidase

A sensitive benzothiazole fluorescent probe for the detection of γ‐glutamyl transpeptidase activity and its application

Lysosome-targeting and legumain-triggered 68Ga-labeled probe for enhanced tumor PET imaging

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