Optimized Growth Conditions for Tissue Engineering of Human Cardiovascular Structures

Sp, Hoerstrup; Zünd, Gregor; Am, Schnell; Sa, Kolb; Jf, Visjager; Schoeberlein, Andreina; Turina, Marko

doi:10.1177/039139880002301206

Cited by 29 publications

(15 citation statements)

References 16 publications

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“…Autologous skin [16] and cartilage transplants [17] generated from patient biopsies are already commercially available for reconstructive surgery; heart valves [18] and blood vessels [19] are currently tested clinically. Numerous bioartificial repair tissues including bladder [20], urethra [21], bone [22], and neural tissue [23] are tested preclinically for potential human applications.…”

Section: Bioartificial Tissue Implants In Reconstructive Surgerymentioning

confidence: 99%

The Potential of Bioartificial Tissues in Oncology Research and Treatment

et al. 2007

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This review article addresses the relevance and potential of bioartificial tissues in oncologic research and therapy and reconstructive oncologic surgery. In order to translate the findings from basic cellular research into clinical applications, cell-based models need to recapitulate both the 3D organization and multicellular complexity of an organ but at the same time accommodate systematic experimental intervention. Here, tissue engineering, the generation of human tissues and organs in vitro, provides new perspectives for basic and applied research by offering 3D tissue cultures resolving fundamental obstacles encountered in currently applied 2D and 3D cell culture systems. Tissue engineering has already been applied to create replacement structures for reconstructive surgery. Applied in vitro, these complex multicellular 3D tissue cultures mimic the microenvironment of human tissues. In contrast to the currently available cell culture systems providing only limited insight into the complex interactions in tissue differentiation, carcinogenesis, angiogenesis and the stromal reaction, the more realistic (micro)environment afforded by the bioartificial tissuespecific 3D test systems may accelerate the progress in design and development of cancer therapies.

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Section: Bioartificial Tissue Implants In Reconstructive Surgerymentioning

confidence: 99%

The Potential of Bioartificial Tissues in Oncology Research and Treatment

et al. 2007

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“…Many investigators have described specific in vitro conditions for tissue development, but the ideal conditions are unknown so far. 13,17 It has been widely confirmed that optimized cell distribution on polymeric scaffolds, an efficient cell culture medium, and exposure to several stimuli such as chemical and mechanical signals may be beneficial for tissue engineering cardiovascular constructs. 18 Currently used cell-seeding procedures for creating heart valves are based on static conditions with the cell suspension being dripped onto the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Short-Term Culture of Human Neonatal Myofibroblasts Seeded Using a Novel Three-Dimensional Rotary Seeding Device

Lueders¹,

Sodian²,

Shakibaei³

et al. 2006

ASAIO Journal

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A crucial factor in the tissue engineering of heart valves is an effective cell seeding with uniform cell distribution on biodegradable scaffolds to eventually form functional tissue constructs in vitro. In our laboratory, we developed a new cell-seeding device for optimal cell distribution for tissue-engineered heart valve constructs. In the present study, we developed a new cell-seeding device made of acrylic glass that is completely transparent (University Hospital Benjamin Franklin, Berlin, Germany). The polymeric heart valve scaffold is fixed in a small-volume, cylindrical cell-seeding chamber, and is surrounded by optimal cell suspension. The cell-seeding chamber is placed in a clear acrylic bowl so that it can be rotated in all directions to provide optimal cell distribution to all areas of the heart valve construct. We thus developed a highly isolated cell-seeding device that is driven by an independently developed rotating machine consisting of two independent motors (University Hospital Benjamin Franklin, Berlin, Germany). The whole system provides a high level of sterility and fits into a humidified incubator. Our newly developed cell-seeding device enables sterile conditions and optimal cell distribution for the controlled fabrication of autologous tissue-engineered heart valve constructs.

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“…[1][2][3][4][5][6] One of these issues is in vitro formation of ideal and viable tissue of autologous human cardiovascular structures before implatation. [2][3][4][5][6]10 Based on the concept of tissue engineering, the prerequisite for in vitro formation of strong, well structured tissue is the appropriate generation of extracellular matrix and cell proliferation. The authors focus on applying the principles of tissue .…”

Section: Discussionmentioning

confidence: 99%

“…22,23 A review of the literature on the cell culture conditions for cell-polymer constructs shows that ascorbic acid is currently used as an additive for improving cardiovascular tissue formation in several laboratories. 10,11 This article presents joint use of bFGF and ascorbic acid as supplements to standard nutrient medium to improve in vitro formation of mature matrix of tissue engineered cardiovascular structures. The effect of the initial modification of the standard cell culture condition with growth factor and ascorbic acid on tissue development in vitro suggests a promising process to further optimize in vitro conditions for tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Effects of Basic Fibroblast Growth Factor and Transforming Growth Factor-β on Maturation of Human Pediatric Aortic Cell Culture for Tissue Engineering of Cardiovascular Structures

Fu¹,

Sodian²,

Lüders³

et al. 2004

ASAIO Journal

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Optimal in vitro conditions are necessary for the development of a strong, well structured, and functional tissue engineered cardiovascular structure eventually designed for implantation. To further optimize in vitro conditions for cell proliferation and extracellular matrix formation in tissue engineering of cardiovascular structures, in this study, ascorbic acid and growth factors as additives to standard cell culture medium were evaluated for their effect on tissue development in vitro. Biodegradable polymer patches [polyglycolic acid (PGA) coated with poly-4-hydroxybutyrate (P4HB)] were seeded with human pediatric aortic cells and cultured for 7 and 28 days. Group A was cultured with standard medium (DMEM with 10% fetal calf serum and 1% antibiotics) supplemented with ascorbic acid; group B was cultured with standard medium plus ascorbic acid and basic fibroblast growth factor (bFGF); group C was cultured with standard medium adding ascorbic acid and transforming growth factor (TGF). Analysis of the cell seeded polymer constructs included DNA assay, collagen assay, and histologic and immunohistochemical examination for cell proliferation and collagen formation. After 7 and 28 days of culture, group B and group C showed a significantly higher DNA content compared with group A. The addition of bFGF (group B) led to a markedly higher collagen synthesis after 28 days of culture compared with the additives in groups C and A. The histologic and immunohistochemical examination also revealed a more dense, organized tissue development with pronounced matrix protein formation in the tissue engineered structures in group B after 28 days of culture. When seeded on to the polymeric scaffold, human vascular cells proliferate and form organized cell tissue after 28 days of culture. The addition of bFGF and ascorbic acid to the standard medium enhances cell proliferation and collagen synthesis on the biodegradable polymer, which leads to the formation of more mature, well organized tissue engineered structures.

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Optimized Growth Conditions for Tissue Engineering of Human Cardiovascular Structures

Cited by 29 publications

References 16 publications

The Potential of Bioartificial Tissues in Oncology Research and Treatment

The Potential of Bioartificial Tissues in Oncology Research and Treatment

Short-Term Culture of Human Neonatal Myofibroblasts Seeded Using a Novel Three-Dimensional Rotary Seeding Device

Effects of Basic Fibroblast Growth Factor and Transforming Growth Factor-β on Maturation of Human Pediatric Aortic Cell Culture for Tissue Engineering of Cardiovascular Structures

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