Optimized grafting of antimicrobial peptides on stainless steel surface and biofilm resistance tests

Héquet, Arnaud; Humblot, Vincent; Berjeaud, Jean‐Marc; Pradier, Claire‐Marie

doi:10.1016/j.colsurfb.2011.01.012

Cited by 107 publications

(80 citation statements)

References 66 publications

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“…Owing to a simple and straightforward method of synthesis, on functionalization, the AMP was found to retain its antibacterial property, which was a problem as reported in a recent study. 44 The capping of polymyxin B on the SNPs enhanced the antimicrobial effectiveness of the nanoparticles. The extant knowledge about the synergistic mode of action of SNPs along with polymyxin B is well established.…”

Section: Resultsmentioning

confidence: 99%

“…LPS is also known as endotoxin, which is shed by Gram-negative bacteria and is notorious for causing sepsis and septic shock. 44 Preservation of the biological activity of polymyxin B upon capping on SNPs prompted us to investigate the ability of such biofunctionalized nanoparticles to remove endotoxins from solutions using a conventional chromogenic Limulus Amebocyte Lysate endotoxin assay ( Figure 7). The PBSNPs efficiently removed endotoxin from the test samples (~97%) as compared with control (~16%).…”

Section: Removal Of Endotoxin By Pbsnpsmentioning

confidence: 99%

See 1 more Smart Citation

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Lambadi¹,

Sharma²,

Kumar³

et al. 2015

IJN

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Infectious diseases cause a huge burden on healthcare systems worldwide. Pathogenic bacteria establish infection by developing antibiotic resistance and modulating the host’s immune system, whereas opportunistic pathogens like Pseudomonas aeruginosa adapt to adverse conditions owing to their ability to form biofilms. In the present study, silver nanoparticles were biofunctionalized with polymyxin B, an antibacterial peptide using a facile method. The biofunctionalized nanoparticles (polymyxin B-capped silver nanoparticles, PBSNPs) were assessed for antibacterial activity against multiple drug-resistant clinical strain Vibrio fluvialis and nosocomial pathogen P. aeruginosa . The results of antibacterial assay revealed that PBSNPs had an approximately 3-fold higher effect than the citrate-capped nanoparticles (CSNPs). Morphological damage to the cell membrane was followed by scanning electron microscopy, testifying PBSNPs to be more potent in controlling the bacterial growth as compared with CSNPs. The bactericidal effect of PBSNPs was further confirmed by Live/Dead staining assays. Apart from the antibacterial activity, the biofunctionalized nanoparticles were found to resist biofilm formation. Electroplating of PBSNPs onto stainless steel surgical blades retained the antibacterial activity against P. aeruginosa . Further, the affinity of polymyxin for endotoxin was exploited for its removal using PBSNPs. It was found that the prepared nanoparticles removed 97% of the endotoxin from the solution. Such multifarious uses of metal nanoparticles are an attractive means of enhancing the potency of antimicrobial agents to control infections.

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Section: Resultsmentioning

confidence: 99%

Section: Removal Of Endotoxin By Pbsnpsmentioning

confidence: 99%

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Lambadi¹,

Sharma²,

Kumar³

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…It is possible that the antimicrobial activity against the isolates of this study has occurred in a similar way, because both are Gram-positive. In recent years, the use of antimicrobial peptides on surfaces to prevent microbial adhesion proves to be an interesting approach in food processing facilities (Massani et al 2008, Aminov 2010, Héquet et al 2011. Bioconservatives molecules such as nisin, pediocin and lauricidin have potential for biofilm control in processing facilities.…”

Section: Discussionmentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

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“…Different types of techniques could be used such as attenuated total reflectance-Fourier transform infrared (FTIR-ATR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), 3D profilometry, X-ray photoelectron spectroscopy (XPS) and timeof-flight secondary ion mass spectrometry (ToF-SIMS) in static and dynamic modes. Recent studies focused on the surface characterization, especially on the interface between chitosan coating and metal support [26,29]. Nevertheless up to now, no study combining both bulk and outermost surface characterization techniques presented above were reported in the literature.…”

Section: Introductionmentioning

confidence: 93%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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Optimized grafting of antimicrobial peptides on stainless steel surface and biofilm resistance tests

Cited by 107 publications

References 66 publications

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

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