Optimized Gas Chromatography-Tandem Mass Spectrometry for 1,1′-sulfonylbis[2-(methylthio) ethane] Quantification in Human Urine

Eyison, Rusen Koray; Sezigen, Sermet; Ortatatli, Mesut; Kenar, Levent

doi:10.1093/chromsci/bmz017

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…These symptoms were observed in our patients on 18th hour after the exposure as shown in Figure 1 and 2. Urine samples were collected on 48th h after the exposure for further diagnosis and these samples were analyzed by using an analytical method that was modified for the quantitation of urinary SBMTE (21). The limit of detection (LOD) for SBMTE was 1 ng/mL and the retention time for SBMTE was about 8.82 min (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

“…We also preferred analyzing urinary SBMTE for the retrospective detection of SM exposure in our cases. Our modified method depended on reducing polar ß-lyase metabolites MSMTESE and SBMSE by using titanium trichloride to low polar ß-lyase metabolite SBMTE which had more suitable chemical structure for GC-MS-MS analysis (1,21).…”

Section: Discussionmentioning

confidence: 99%

“…An analytical method which was reported previously was used for the sample pretreatment and SPE (21). Urine sample (500 µL) was mixed with 12% TiCl3 solution (250 µL) and the solution was heated at 75º C for 60 minutes (min) for the complete reduction of MSMTESE and SBMSE to SBMTE.…”

Section: Methodsmentioning

confidence: 99%

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Bioanalytical verification of sulfur mustard exposure in a syrian family

Sezigen¹,

Eyison²,

Kenar³

2019

Gulhane Med J

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Aims:Sulfur mustard (SM) is a well-known chemical warfare agent. It is a highly reactive compound which has cytotoxic and carcinogenic. 1,1'-sulfonylbis[2-(methylthio)ethane] (SBMTE) is a ß-lyase metabolite of SM and it is the unequivocal diagnostic biomarker for SM exposure. Our study aimed to confirm SM exposure by detecting quantitative levels of SBMTE in urine samples of a family of four. Methods:A family of four who was exposed to SM was included into the study. Urine samples of victims were analysed by using a gas chromatography-tandem mass spectrometry (GC-MS-MS) method for the detection of urinary SBMTE levels. External calibration standards were used in the multiple reaction monitoring mode of GC-MS-MS. Results:The limit of detection (LOD) for SBMTE was 1 ng/mL and the retention time for SBMTE was about 8.82 min. Urinary SBMTE levels of Patient 1 and 2 were measured as 314.43 ng/mL and 61.57 ng/mL respectively. Conclusions:We confirmed SM exposure in urine samples of two patients by quantitative analysis of urinary SBMTE. The retrospective detection of urinary SBMTE supported SM exposure which was initially diagnosed with typical medical history, and presented typical symptoms.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bioanalytical verification of sulfur mustard exposure in a syrian family

Sezigen¹,

Eyison²,

Kenar³

2019

Gulhane Med J

Self Cite

View full text Add to dashboard Cite

show abstract

“…[5][6][7][8][9][10] However, the methods for analysing protein and DNA adducts of NMs are preferable as they are stable from several weeks to months after exposure. 11,12 For sulphur mustard (SM), several methods were developed to determine β-lyase metabolites [13][14][15][16][17] for urine samples. Therefore, one option to detect NMs in biomedical samples is to study their conjugation with GSH.…”

Section: Introductionmentioning

confidence: 99%

Glutathione conjugation of nitrogen mustards: In vitro study

Hamzah

Kjellberg

Vanninen

2023

Rapid Comm Mass Spectrometry

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Rationale: This paper describes an in vitro study designed to identify metabolic biomarkers resulting from the conjugation of nitrogen mustards (NMs) with glutathione (GSH). The method developed is essential in providing evidence in the event of NM exposure in biomedical samples.Methods: The mass spectral characterization of the proposed NMs-GSH conjugates was performed with liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS/MS). The final reaction mixtures were analysed in positive electrospray ionisation (ESI) at different incubation times.Results: This study identified three types of conjugates in addition to ethanolamines, the hydrolysis products of NMs. Monoglutathionyl, diglutathionyl and phosphorylated conjugates were produced for each of the NMs, bis(2-chloroethyl) ethylamine (HN1), bis(2-chloroethyl)methylamine (HN2) and tris(2-chloroethyl)amine (HN3). The monoglutathionyl conjugates consisted of HN1-GSH, HN2-GSH and HN3-GSH. The spontaneous and primary conjugates of diglutathionyl were HN1-GSH2, HN2-GSH2 and HN3-GSH2. These included phosphorylated conjugates, namely HN1-GSH-PO 4 , HN2-GSH-PO 4 and HN3-GSH-PO 4 , as might have formed due to hydrolysis in phosphate buffer. Conclusions:The mass spectral data of all conjugates formed in the presence of all NMs and GSH are reported in this study. These GSH metabolites can be used to confirm NMs toxicity in biological samples such as urine.

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Biomarkers of Sulfur Mustard (Mustard Gas) in Urine

Sezigen

2022

Biomarkers in Toxicology

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Optimized Gas Chromatography-Tandem Mass Spectrometry for 1,1′-sulfonylbis[2-(methylthio) ethane] Quantification in Human Urine

Cited by 10 publications

References 16 publications

Bioanalytical verification of sulfur mustard exposure in a syrian family

Bioanalytical verification of sulfur mustard exposure in a syrian family

Glutathione conjugation of nitrogen mustards: In vitro study

Biomarkers of Sulfur Mustard (Mustard Gas) in Urine

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