Abstract:Second near-infrared (NIR-II, 1000-1700 nm) photon-mediated fluorescence imaging has attracted extensive interest in the field of bioimaging. However, NIR-II fluorescent nanoprobes competent for plant imaging have been rarely developed. Herein,...
“…1E , the prepared UCNP emits strong red light at 654 nm under 980 nm excitation (black line), which can be attributed to the 4 F 9/2 – 4 I 15/2 transitions of Er 3+ . 18 While TMBox has a wide UV-vis absorption spectrum at 550–750 nm (red line), which overlaps well with the red light emitted by UCNP and can effectively quench the fluorescence of UCNP. Therefore, a sensing platform for detecting CHOL is constructed using TMBox as the energy receptor and UCNP as the energy donor.…”
Cholesterol (CHOL) is an important clinical biochemical indicator that plays an important role in the regulation of the fluidity, permeability, and microstructure of cell membranes.
“…1E , the prepared UCNP emits strong red light at 654 nm under 980 nm excitation (black line), which can be attributed to the 4 F 9/2 – 4 I 15/2 transitions of Er 3+ . 18 While TMBox has a wide UV-vis absorption spectrum at 550–750 nm (red line), which overlaps well with the red light emitted by UCNP and can effectively quench the fluorescence of UCNP. Therefore, a sensing platform for detecting CHOL is constructed using TMBox as the energy receptor and UCNP as the energy donor.…”
Cholesterol (CHOL) is an important clinical biochemical indicator that plays an important role in the regulation of the fluidity, permeability, and microstructure of cell membranes.
“…Fluorescent labelling combined with staining is an evergreen research field because of its intrinsic sensitivity, high spatiotemporal resolution, non-invasion, etc. 40,41 Aiming to achieve the proof-of-concept objectives, cellular experiments of the SQs utilizing the model of onion epidermal cells were first assessed. Rapid cellular uptake of the SQs and notable differences in their internalization and organelle distribution were illustrated (Fig.…”
Organelle-selective vision provides insights into the physiological response of plants and crops to environmental stresses in sustainable agriculture ecosystems. Biological applications often require two-photon excited fluorophores with low phototoxicity, high...
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