Optimized combined electrical–chemical parthenogenetic activation for in vitro matured bovine oocytes

Hosseini, Seyed Morteza; Hajian, Mehdi; Moulavi, F.; Shahverdi, Abdolhossein; Nasr-Esfahani, Mohammad Hossein

doi:10.1016/j.anireprosci.2007.07.011

Cited by 31 publications

(28 citation statements)

References 46 publications

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“…We found that combined activation significantly improved the development potential of parthenogenetic embryos than chemical activation or electrical activation only. It was consistent with the previous studies in cattle (Hosseini et al 2008), goat (Kumar et al 2014), and pig (Ock et al 2011). Comparing with Ion chemical treatment, combined treatment of electrical stimuli induced more Ca 2+ oscillations, and it could mimic intracellular events induced by sperm penetration more efficiently (Sun et al 1992).…”

Section: Discussionsupporting

confidence: 91%

“…Combined activation which integrated a transient inactivation of MPF obtainable with a single Ca 2+ rise, and with a persistent inhibition of MPF, induced by addition of either protein synthesis inhibitors or nonspecific kinase inhibitors may further improve the developmental potential of the IVM oocytes. IVM oocytes with the electrical stimuli or combined activation stimuli resulted in high embryo development in several species (Zhu et al 2002;Hosseini et al 2008;Hai et al 2014). Despite extensive research in this area, none of the available studies have been done to systematically compare chemical, electrical, and combined activation protocols, and less attention has been paid to the sequential use of these Ca 2+ -immobilizing agents, which may benefit the activation outcome.…”

Section: Introductionmentioning

confidence: 97%

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Comparison of chemical, electrical, and combined activation methods for in vitro matured porcine oocytes

Liu

Cui

et al. 2014

In Vitro Cell.Dev.Biol.-Animal

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Factors influencing porcine oocyte activation were systematically studied. This study included (1) the effect of ionomycin plus various chemical agents on activation, (2) comparison of different electrical activation parameters, (3) optimization of combined activation, and (4) evaluation of the optimized protocols. The results showed that (1) blastocyst rates of ionomycin (Ion) + 6-dimethylaminopurine (6-DMAP) (29.7 ± 1.1%), Ion + cytochalasin B (CB) + cycloheximide (CHX) (29.8 ± 1.2%), Ion + CB + 6-DMAP (30.4 ± 1.6%), and Ion + CB + CHX + 6-DMAP (30.2 ± 2.7%) were significantly higher than Ion + CHX (15.8 ± 1.5%, p < 0.05); (2) the parthenogenetic blastocyst formation of electrical activation was optimal when oocytes were activated by three direct current (DC) pulses of 1.00 kV cm(-1) for 80 μs (39.5 ± 1.1%); (3) blastocyst rates of DC + CB + CHX (55.4 ± 1.2%) and DC + CB + 6-DMAP (50.4 ± 2.9%) were significantly higher than DC + 6-DMAP, DC + CB + CHX + 6-DMAP, electrical activation, and chemical activation alone (p < 0.05); and (4) approximately 84% of parthenogenetic blastocysts yielded by the optimized protocol were diploid, which was significantly higher than that of electrical activation blastocysts (40%). Using the optimized electrical and combined activation protocol, high blastocyst rates were generated by intracytoplasmic sperm injection (ICSI) (34.6 ± 1.1%), cytoplasmic microinjection (CI) (52.3 ± 2.2%), and handmade cloning (HMC) (31.2 ± 1.0%), respectively. This study concludes that the optimal activation protocol of in vitro matured porcine oocytes was combined activation with parameter as three DC pulses of 1.00 kV cm(-1) for 80 μs plus CB and CHX treatment.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Comparison of chemical, electrical, and combined activation methods for in vitro matured porcine oocytes

Liu

Cui

et al. 2014

In Vitro Cell.Dev.Biol.-Animal

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“…The couplets were then electrofused [a sinusoidal electric current (7 V/ cm) for 10 s followed by two direct currents (1.75 kV/cm for 30 μsec and 1 s delay)] in 290 mOsm fusion buffer free of Ca 2+ and Mg 2+ . The reconstructed oocytes were activated using ionomycin (5 μM, 5 min) followed by incubation with 2 mM 6-dimethyl aminopurine for 4 h [17]. Activated reconstructs were cultured in presence (SCNT-TSA) or absence (SCNT) of 50 nM TSA for 12 h and then cultured up to 8 days as described by Vajta et al [18].…”

Section: Somatic Cell Nuclear Transfer (Scnt)mentioning

confidence: 99%

Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos

Jafari

Hosseini

Hajian

et al. 2011

J Assist Reprod Genet

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“…To rescue cases that encounter problems in oocyte activation immediately following sperm-oocyte fusion, certain artificial activation procedures have been recommended: mechanical activation by a modified ICSI technique [8,9], electrical activation [10,11], chemical compounds such as strontium chloride [12,13], ionomycin [14], and calcium ionophores [15], and sequential treatment with calcium ionophore and puromycin [16].…”

Section: Introductionmentioning

confidence: 99%

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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Purpose Fertilization failures have occurred repeatedly in reproductive centers after intracytoplasmic sperm injection (ICSI) and artificial oocyte activation (AOA) has been used to prevent it. This study was performed to investigate whether spermatozoan origin influences clinical outcomes of AOAwith a calcium ionophore. Methods A total of 185 ICSI cycles with a history of no or low fertilization was included in this retrospective study. The outcomes of AOA after ICSI were compared with ejaculatednormal, ejaculated-oligo-astheno-terato or extracted-testicular spermatozoa. Results There were significant differences between the previous standard ICSI cycles and AOA cycles in the rate of fertilization and clinical outcomes among cases with different sperm origins. Thirty-eight healthy babies (20 singles and 18 twins, 29 cycles) were successfully delivered, and no congenital birth defects were observed. Conclusions Most patients with a no or low fertilization history obtained an increased fertilization rate and a positive clinical outcome with AOA regardless of the origin of spermatozoa.

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Optimized combined electrical–chemical parthenogenetic activation for in vitro matured bovine oocytes

Cited by 31 publications

References 46 publications

Comparison of chemical, electrical, and combined activation methods for in vitro matured porcine oocytes

Comparison of chemical, electrical, and combined activation methods for in vitro matured porcine oocytes

Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

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