Optimized ARROW-Based MMI Waveguides for High Fidelity Excitation Patterns for Optofluidic Multiplexing

Stott, Matthew A.; Ganjalizadeh, Vahid; Olsen, Maclain; Orfila, Marcos; McMurray, Johnny; Schmidt, Holger; Hawkins, Aaron R.

doi:10.1109/jqe.2018.2816120

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…The side illuminated biosensor excited passing particles with a seven-spot multi-mode interferometer (MMI) waveguide, used for signal multiplexing [24]. For testing, fluorescent beads (ThermoFisher Scientific ® FluoSpheres™ 625/645 Crimson) 0.2 µm in diameter were used as targets as a stand-in for bioparticles.…”

Section: Figurementioning

confidence: 99%

Performance Comparison of Flow-Through Optofluidic Biosensor Designs

et al. 2021

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Optofluidic flow-through biosensors are being developed for single particle detection, particularly as a tool for pathogen diagnosis. The sensitivity of the biosensor chip depends on design parameters, illumination format (side vs. top), and flow configuration (parabolic, two- and three-dimensional hydrodynamic focused (2DHF and 3DHF)). We study the signal differences between various combinations of these design aspects. Our model is validated against a sample of physical devices. We find that side-illumination with 3DHF produces the strongest and consistent signal, but parabolic flow devices process a sample volume more quickly. Practical matters of optical alignment are also discussed, which may affect design choice.

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Section: Figurementioning

confidence: 99%

Performance Comparison of Flow-Through Optofluidic Biosensor Designs

et al. 2021

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“…Although these devices can detect several emission wavelengths without optical filters, spectral resolution is limited, which compromises accuracy. Light-guiding components are used to prevent the excitation light from reaching the detectors due to the collimation of excitation light and isotropy of emission light [12,13]. Attenuation of excitation can be achieved by free-space optical elements without thin film filters, but non-idealities in fabrication can severely affect attenuation efficiency.…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost Time-Correlated Single Photon Counting Portable DNA Analyzer

Tian

Wei

2019

Sensors

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Photon-counting analysis of nucleic acids plays a key role in many diagnostics applications for its accurate and non-invasive nature. However, conventional photon-counting instrumentations are bulky and expensive due to the use of conventional optics and a lack of optimization of electronics. In this paper, we present a portable, low-cost time-correlated single photon-counting (TCSPC) analysis system for DNA detection. Both optical and electronic subsystems are carefully designed to provide effective emission filtering and size reduction, delivering good DNA detection and fluorescence lifetime extraction performance. DNA detection has been verified by fluorescence lifetime measurements of a V-carbazole conjugated fluorophore lifetime bioassay. The time-to-digital module of the proposed TCSPC system achieves a full width at half maximum (FWHM) timing resolution from 121 to 145 ps and a differential non-linearity (DNL) between −8.5% and +9.7% of the least significant bit (LSB) within the 500 ns full-scale range (FSR). With a detection limit of 6.25 nM and a dynamic range of 6.8 ns, the proposed TCSPC system demonstrates the enabling technology for rapid, point-of-care DNA diagnostics.

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“…Cross-polarizers have been employed to filter excitation [16]. Micro waveguides have been used to separately propagate excitation and emission [17], [18].…”

Section: Introductionmentioning

confidence: 99%

“…This unique advantage offers vast potential for the development of lowcost microsystems for fluorescence detection. In the last several years, optofluidic platforms based on the LCW have been investigated for different applications, such as for low concentration toxic gases detection [19], [23], real-time single molecule detection [24], flowing micro droplet based absorbance detection [18], and single-virus detection [17], [25].…”

Section: Introductionmentioning

confidence: 99%

Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing

et al. 2019

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Time-resolved fluorescence is a widely adopted technique for DNA detection due to its high sensitivity and selectivity. However, due to stringent requirements on optics and electronics, instrumentation with time-resolved capability is bulky and expensive, prohibiting their use in portable and point-of-care applications. In this work, a fully-integrated DNA biosensor based on liquid-core waveguide (LCW) optics for fluorescence lifetime analysis is presented. The DNA biosensor encompasses all-custom bioassay, optics and electronics into a microsystem, delivering a near sample-to-answer level of integration. Lifetime, rather than intensity, is exploited as the analytical signal from the V-carbazole probe for the first time. Excitation propagation within the LCW is investigated both analytically and in simulations to achieve high excitation rejection and low temporal dispersion, enabling the proposed LCW-based system with much smaller instrumentation size to deliver comparable lifetime measurement accuracy to traditional systems. Detection of DNA down to 15 base pairs at a low detection limit of 1.38 nM demonstrates the high applicability of the proposed biosensor for compact, application-specific, and low-cost diagnostics devices.INDEX TERMS DNA biosensing, fluorescence lifetime detection, liquid-core waveguide, TCSPC, turn-on fluorescent probe.

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Optimized ARROW-Based MMI Waveguides for High Fidelity Excitation Patterns for Optofluidic Multiplexing

Cited by 10 publications

References 23 publications

Performance Comparison of Flow-Through Optofluidic Biosensor Designs

Performance Comparison of Flow-Through Optofluidic Biosensor Designs

A Low-Cost Time-Correlated Single Photon Counting Portable DNA Analyzer

Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing

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