Optimized architecture for Tyrosinase-containing Langmuir–Blodgett films to detect pyrogallol

Pavinatto, Felippe J.; Fernandes, Edson Giuliani Ramos; Aléssio, Priscila; Constantino, Carlos J. L.; Saja, J.A. de; Zucolotto, Valtencir; Apetrei, Constantin; Oliveira, Osvaldo N.; Rodrı́guez-Méndez, M.L.

doi:10.1039/c0jm03864d

Cited by 55 publications

(45 citation statements)

References 65 publications

(63 reference statements)

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“…One of the objectives of this work was to improve the selectivity of the sensors by introducing enzymes in biomimetic layers containing LuPc 2 as electron mediator Pavinatto et al, 2011;Yin et al, 2009). Two phenoloxidases (tyrosinase and laccase) were selected.…”

Section: Electrochemical Response Towards Phenolsmentioning

confidence: 99%

Bioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapes

Medina-Plaza

Saja

Rodrı́guez-Méndez

2014

Biosensors and Bioelectronics

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a b s t r a c tIn this work, a multisensor system formed by nanostructured voltammetric biosensors based on phenol oxidases (tyrosinase and laccase) has been developed. The enzymes have been incorporated into a biomimetic environment provided by a Langmuir-Blodgett (LB) film of arachidic acid (AA). Lutetium bisphthalocyanine (LuPc 2 ) has also been introduced in the films to act as electron mediator. The incorporation of the enzymes to the floating layers to form Tyr/AA/LuPc 2 and Lac/AA/LuPc 2 films has been confirmed by the expansion in the surface pressure isotherms and by the AFM images. The voltammetric response towards six phenolic compounds demonstrates the enhanced performance of the biosensors that resulted from a preserved activity of the tyrosinase and laccase combined with the electron transfer activity of LuPc 2 . Biosensors show improved detection limits in the range of 10. An array formed by three sensors AA/LuPc 2 , Tyr/AA/LuPc 2 and Lac/AA/LuPc 2 has been employed to discriminate phenolic antioxidants of interest in the food industry. The Principal Component Analysis scores plot has demonstrated that the multisensor system is able to discriminate phenols according to the number of phenolic groups attached to the structure. The system has also been able to discriminate grapes of different varieties according to their phenolic content. This good performance is due to the combination of four factors: the high functionality of the enzyme obtained using a biomimetic immobilization, the signal enhancement caused by the LuPc 2 mediator, the improvement in the selectivity induced by the enzymes and the complementary activity of the enzymatic sensors demonstrated in the loading plots.

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Section: Electrochemical Response Towards Phenolsmentioning

confidence: 99%

Bioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapes

Medina-Plaza

Saja

Rodrı́guez-Méndez

2014

Biosensors and Bioelectronics

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“…However, for some cases, the enzyme activity could be even higher than that calculated for the homogeneous environment, which is the case where the catalytic site is a heme group instead of a polypeptide moiety of the enzyme (Schmidt et al 2008, Goto et al 2010. Other examples involve also the use of enzyme based LB films for sensor purposes as an electronic tongue (Pavinatto et al 2011).…”

mentioning

confidence: 99%

“…This strategy was applied for the deposition of biocatalysts with active layers of penicillin G acylase (PGA) (Pastorino et al 2002) which preserved an enzyme widely used for medicine production. Also, we can cite: (iv) galactose oxidase Langmuir-Blodgett (LB) films of poly(3-hexyl thiophene) (P3HT)/stearic acid (SA) for the estimation of lactose in milk and its products to prevent "lactose intolerance" (Zheng et al 2004) where the stability was observed for 120 days; (v) laccase and tyrosinase mixed with octadecyltrimethylammonium bromide and with linoleic acid to detect cathecol (Cabaj et al 2010); (vi) cholesterol oxidase immobilized in LB films consisting of positively charged octadecyltrimethylammonium and nano-sized Prussian-blue clusters (Ohnuki et al 2009); (vii) a uricase-chitosan -n-nonadecanoic acid LB films to detect uric acid ; (viii) Hyaluronidase-phospholipid LB films designed to determine hyaluronic acid by using fluorescence spectroscopy (Monteiro et al 2011); (ix) tyrosinase incorporated in a lipidic layer and the use of lutetium bisphthalocyanine as an electron mediator for the voltammetric detection of phenol derivatives, which include one monophenol (vanillic acid), two diphenols (catechol and caffeic acid) and two triphenols (gallic acid and pyrogallol) (Apetrei et al 2012) (x) a bioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases for the analysis of grapes by means of amperometric and capacitance measurements (Pavinatto et al 2011); and (xi) malate dehydrogenase-cationic lipid systems to determine malic acid (Gur et al 2016). Table I summarizes some highlights on the development of bionsensors based on LB films reported in the last three decades.…”

mentioning

confidence: 99%

Enzymes immobilized in Langmuir-Blodgett films: Why determining the surface properties in Langmuir monolayer is important?

Caseli

2018

An. Acad. Bras. Ciênc.

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In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980's, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device.

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“…Several kinds of sensing elements and detection methods have been studied for e-noses and mainly e-tongues [45,51,[57][58][59][60][61][62], which allow applicability in fields as food [57,[62][63][64][65][66], wines [67], water [68] and pharmaceutical analysis [66]. The importance of the e-tongues and e-noses to biosensing stems from the possible extension through the incorporation of sensing units capable of molecular recognition [69][70][71][72].…”

Section: Electronic Tongues and Nosesmentioning

confidence: 99%

Information Visualization to Enhance Sensitivity and Selectivity in Biosensing

Oliveira¹,

Pavinatto²,

Constantino³

et al. 2012

Biointerphases

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An overview is provided of the various methods for analyzing biosensing data, with emphasis on information visualization approaches such as multidimensional projection techniques. Emphasis is placed on the importance of data analysis methods, with a description of traditional techniques, including the advantages and limitations of linear and non-linear methods to generate layouts that emphasize similarity/dissimilarity relationships among data instances. Particularly important are recent methods that allow processing high-dimensional data, thus taking full advantage of the capabilities of modern equipment. In this area, now referred to as e-science, the choice of appropriate data analysis methods is crucial to enhance the sensitivity and selectivity of sensors and biosensors. Two types of systems deserving attention in this context are electronic noses and electronic tongues, which are made of sensor arrays whose electrical or electrochemical responses are combined to provide “finger print” information for aromas and tastes. Examples will also be given of unprecedented detection of tropical diseases, made possible with the use of multidimensional projection techniques. Furthermore, ways of using these techniques along with other information visualization methods to optimize biosensors will be discussed.

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Optimized architecture for Tyrosinase-containing Langmuir–Blodgett films to detect pyrogallol

Cited by 55 publications

References 65 publications

Bioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapes

Bioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapes

Enzymes immobilized in Langmuir-Blodgett films: Why determining the surface properties in Langmuir monolayer is important?

Information Visualization to Enhance Sensitivity and Selectivity in Biosensing

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