Optimized application of surface-enhanced laser desorption/ionization time-of-flight MS to differentiate<i>Francisella tularensis</i>at the level of subspecies and individual strains

Seibold, Eric; Bogumil, Ralf; Vorderwülbecke, Sonja; Dahouk, Sascha Al; Buckendahl, A.; Tomaso, Herbert; Splettstoesser, Wolf D.

doi:10.1111/j.1574-695x.2007.00216.x

Cited by 29 publications

(14 citation statements)

References 29 publications

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“…This method, enhanced using in addition a hydrophobic affinity surface, resulted in the possibility of analyzing F. tularensis strains within the sub-species discrimination (Seibold et al, 2007). Schmid et al (2005) were able to distinguish 350 extracts of Neisseria gonorrhoeae from closely related species with a sensitivity of 95.7% and a specificity of 97.1% after the SELDI-MS using hydrophobic ProteinChips.…”

Section: F Sample Fractionationmentioning

confidence: 98%

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Sample preparation methods for MALDI‐MS profiling of bacteria

Šedo¹,

Sedláček²,

Zdráhal³

2010

Mass Spectrometry Reviews

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Direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.

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Section: F Sample Fractionationmentioning

confidence: 98%

“…Section II.F) As an nice example could be mentioned distinguishing F. tularensis strains by means of SELDI-MS (Lundquist et al, 2005;Seibold et al, 2007) or the increase of MALDI-MS profile informativeness resulting in …”

Section: Discriminatory Powermentioning

confidence: 99%

Sample preparation methods for MALDI‐MS profiling of bacteria

Šedo¹,

Sedláček²,

Zdráhal³

2010

Mass Spectrometry Reviews

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“…The available literature regarding the identification of and discrimination between subspecies or even single strains by MALDI-TOF MS is limited (1,24). Francisella tularensis subspecies were successfully discriminated with surface-enhanced laser desorption ionization-time of flight MS (SELDI-TOF MS) (19,26), a modified MALDI-TOF MS method which allows selective absorption of proteins on a chromatographic array surface prior to MS (31,32). Compared to SELDI-TOF MS, MALDI-TOF MS is less time-consuming and more cost-effective.…”

mentioning

confidence: 99%

Identification of Francisella tularensis by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Fast, Reliable, Robust, and Cost-Effective Differentiation on Species and Subspecies Levels

et al. 2010

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Francisella tularensis, the causative agent of tularemia, is a potential agent of bioterrorism. The phenotypic discrimination of closely related, but differently virulent, Francisella tularensis subspecies with phenotyping methods is difficult and time-consuming, often producing ambiguous results. As a fast and simple alternative, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was applied to 50 different strains of the genus Francisella to assess its ability to identify and discriminate between strains according to their designated species and subspecies. Reference spectra from five representative strains of Francisella philomiragia, Francisella tularensis subsp. tularensis, Francisella tularensis subsp. holarctica, Francisella tularensis subsp. mediasiatica, and Francisella tularensis subsp. novicida were established and evaluated for their capability to correctly identify Francisella species and subspecies by matching a collection of spectra from 45 blind-coded Francisella strains against a database containing the five reference spectra and 3,287 spectra from other microorganisms. As a reference method for identification of strains from the genus Francisella, 23S rRNA gene sequencing was used. All strains were correctly identified, with both methods showing perfect agreement at the species level as well as at the subspecies level. The identification of Francisella strains by MALDI-TOF MS and subsequent database matching was reproducible using biological replicates, different culture media, different cultivation times, different serial in vitro passages of the same strain, different preparation protocols, and different mass spectrometers.

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“…Recently, after sequencing of the genomes of individual F. tularensis subspecies, mass spectrometric identification of F. tularensis and its typing into subspecies and even into individual strains has become possible [201][202][203][204]. Another possibility for precisely identifying F. tularensis isolates consists in automated genotyping assay based on the analysis of variable number tandem repeat (VNTR) or multiple loci VNTR analysis (MLVA) markers [205,206].…”

Section: Diagnosis Detection and Laboratory Confirmationmentioning

confidence: 99%

Putting the Jigsaw Together - A Brief Insight Into the Tularemia

Kubelková

Macela

2015

Open Life Sciences

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Tularemia is a debilitating febrile and potentially fatal zoonotic disease of humans and other vertebrates caused by the Gram-negative bacterium Francisella tularensis. The natural reservoirs are small rodents, hares, and possibly amoebas in water. The etiological agent, Francisella tularensis, is a non-spore forming, encapsulated, facultative intracellular bacterium, a member of the γ-Proteobacteria class of Gram-negative bacteria. Francisella tularensis is capable of invading and replicating within phagocytic as well as non-phagocytic cells and modulate inflammatory response. Infection by the pulmonary, dermal, or oral routes, respectively, results in pneumonic, ulceroglandular, or oropharyngeal tularemia. The highest mortality rates are associated with the pneumonic form of this disease. All members of Francisella tularensis species cause more or less severe disease Due to their abilities to be transmitted to humans via multiple routes and to be disseminated via biological aerosol that can cause the disease after inhalation of even an extremely low infectious dose, Francisella tularensis has been classified as a Category A bioterrorism agent. The current standard of care for tularemia is treatment with antibiotics, as this therapy is highly effective if used soon after infection, although it is not, however, absolutely effective in all cases.

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Optimized application of surface-enhanced laser desorption/ionization time-of-flight MS to differentiateFrancisella tularensisat the level of subspecies and individual strains

Cited by 29 publications

References 29 publications

Sample preparation methods for MALDI‐MS profiling of bacteria

Sample preparation methods for MALDI‐MS profiling of bacteria

Identification of Francisella tularensis by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Fast, Reliable, Robust, and Cost-Effective Differentiation on Species and Subspecies Levels

Putting the Jigsaw Together - A Brief Insight Into the Tularemia

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