Optimized Antibiotic Resistance Genes Monitoring Scenarios Promote Sustainability of Urban Water Cycle
Zhou-Hua Cheng,
Xi-Yan Luo,
Dong-Feng Liu
et al.
Abstract:Dissemination
of antibiotic resistance genes (ARGs) in urban water
bodies has become a significant environmental and health concern.
Many approaches based on real-time quantitative PCR (qPCR) have been
developed to offer rapid and highly specific detection of ARGs in
water environments, but the complicated and time-consuming procedures
have hindered their widespread use. Herein, we developed a facile
one-step approach for rapid detection of ARGs by leveraging the trans-cleavage activity of Cas12a and recombina… Show more
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