Optimized Analysis of Organic Acids in Edible Mushrooms from Portugal by Ultra Fast Liquid Chromatography and Photodiode Array Detection

Abstract

Organic acid profiles of different mushroom species were obtained by ultra-fast liquid chromatography (UFLC), by means of photodiode array detector (PDA). The chromatographic separation was achieved using a SphereClone (Phenomenex) reverse phase C 18 column using an isocratic elution with sulphuric acid (3.6 mM) at a flow rate of 0.8 mL/min. All the compounds were separated in 8 min. The method was optimized usingAgaricus bisporus sample and proved to be reproducible and accurate. Organic acid profiles were quite homogeneous for all mushroom samples; oxalic, malic and fumaric acids were the main organic acids; some samples also presented quinic and citric acids.Sarcondon imbricatus was the species that presented the highest total content (254.09 mg/g dw), while Bovista nigrescens presented the lowest concentration (1.33 mg/g dw). The high amounts of organic acids present in all the species may suggest that they could be related to the antioxidant activity found in these species and previously reported by us.

Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal “mushroom”

“…Organic acids were determined by ultra fast liquid chromatography (UFLC, Shimadzu 20A series) coupled with a photodiode array detector (PDA), after dissolving each extract in metaphosphoric acid (4%), at a known concentration (Barros et al, 2013). The Phenolic compounds.…”

Study on chemical, bioactive and food preserving properties ofLaetiporus sulphureus(Bull.: Fr.) Murr.

“…Organic acids were determined following a procedure previously optimized and described by the authors. 17 Analysis was performed by an ultra-fast liquid chromatograph (UFLC) coupled with a photodiode array detector (PDA), using a Shimadzu 20A series UFLC (Shimadzu Corporation). Detection was carried out in a PDA, using 215 nm and 245 nm as preferred wavelengths.…”

Bioactivity and phytochemical characterization of Arenaria montana L.

“…Organic acids were determined following a procedure previously described by the authors. 24 The analysis was performed using a Shimadzu 20A series UFLC (Shimadzu Coperation, Kyoto, Japan). Separation was achieved using a SphereClone (Phenomenex, Torrance, CA, USA) reverse phase C 18 column (5 mm, 250 mm  4.6 mm i.d) thermostatted at 35 C. The elution was performed with sulphuric acid 3.6 mM using a ow rate of 0.8 mL min À1 .…”