Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (&lt;i&gt;Juglans regia&lt;/i&gt; L.)

Ćelepirović, Nevenka; Vlahović, Maja; Dounavi, Aikaterini; Ivanković, Mladen

doi:10.14214/sf.1674

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…The following sections depict an overview of these molecular studies and their application (Table 2). Woeste et al 2002;Dangl et al 2005;Hoban et al 2008;Zhang et al 2010;Qi et al 2011;Yi et al 2011;Wu et al 2012;Zhang et al 2013;Chen et al 2013;Najafi et al 2014;Topçu et al 2015;Ikhsan et al 2016;Dang et al 2015;Hu et al 2015;Dang et al 2016 Interspecific hybrids identification Pollegioni et al 2008Pollegioni et al , 2009 Genetic diversity/relationships determination Foroni et al 2005;Dangl et al 2005;Victory et al 2006;Robichaud et al 2006;Wang et al 2008;Aradhya et al 2009;Gunn et al 2010;Karimi et al 2010;Bai et al 2010;Mohsenipoor et al 2010;Ebrahimi et al 2011;Ruiz-Garcia et al 2011;Pollegioni et al 2011;Qi et al 2011;Grauke et al 2012;Mahmoodi et al 2013;Zhang et al 2013;Pop et al 2013;Najafi et al 2014;Dang et al 2015;Hu et al 2015;Han et al 2016;Noor Shah et al 2016;Ćelepirović et al 2016;Wang et al 2016;…”

Section: Historical Overview Of Molecular Marker Development and Usementioning

confidence: 99%

Walnut: past and future of genetic improvement

Bernard

Lheureux²,

Dirlewanger

2017

Tree Genetics & Genomes

153

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Persian or English walnut (Juglans regia L.), the walnut species cultivated for nut production, is one of the oldest food sources known. Persian walnuts, native to the mountain valleys of Central Asia, are grown worldwide in temperate areas. World production exceeds three million tons since 2012, mostly provided by China, the USA, and Iran. Despite very ancient culture of walnut species (Juglans spp.), breeding actually started in the twentieth century. Using a range of methodologies, from morphological markers to the most recent advances in genome analysis, many genetic studies of walnut have been conducted during the past 30 years, including examination of diversity, determination of relationships within or among germplasm collections and populations, phylogenetic and origin elucidation, genetic map construction, and biotic or abiotic stress investigations. The genetic improvement of walnut has undergone great evolution. The producing countries of the Middle East have widely studied morphological characteristics of walnut. The USA and France, for example, are behind important cultivar releases such as BChandler^and BFranquette.^Finally, genomics represents a major breakthrough in walnut improvement, in particular by recent sequencing of both chloroplast and nuclear genomes. This review summarizes worldwide molecular and Bomics^studies and gives an overview of the main walnut breeding programs.

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Section: Historical Overview Of Molecular Marker Development and Usementioning

confidence: 99%

Walnut: past and future of genetic improvement

Bernard

Lheureux²,

Dirlewanger

2017

Tree Genetics & Genomes

153

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“…In walnut, microsatellite loci have been developed for different species in previous studies ( Table S1 ) [ 20 , 21 , 22 , 23 ]. However, multiplexing of microsatellite loci has not yet been fully embraced in Juglans , with only a few published studies [ 24 ]. Hence, the goal of the present study was to develop and characterize highly polymorphic SSRs by cross-species amplification and de novo development and ultimately to construct a multiplex PCR system for the loci.…”

Section: Introductionmentioning

confidence: 99%

A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts

Xiahou,

Wambulwa,

et al. 2023

Plants

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Multiplex polymerase chain reaction (PCR) of microsatellite loci allows for simultaneous amplification of two or more pairs of primers in a single PCR reaction; hence, it is cost and time effective. However, very few attempts have been reported in non-model species. In this study, by combining a genome-based de novo development and cross-species application approach, a multiplex PCR system comprising 5 PCR reactions of 33 microsatellites consisting of 26 novel genomic and 7 literature-sourced loci was tested for polymorphisms, cross-species transferability, and the ability to assess genetic diversity and population structure of three walnut species (Juglans spp.). We found that the genome-based approach is more efficient than other methods. An allelic ladder was developed for each locus to enhance consistent genotyping among laboratories. The population genetic analysis results showed that all 33 loci were successfully transferred across the three species, showing high polymorphism and a strong genetic structure. Hence, the multiplex PCR system is highly applicable in walnut species. Furthermore, we propose an efficient pipeline to characterize and genotype polymorphic microsatellite loci. The novel toolbox developed here will aid future ecology and evolution studies in walnut and could serve as a model for other plant species.

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Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)

Cited by 2 publications

References 22 publications

Walnut: past and future of genetic improvement

Walnut: past and future of genetic improvement

A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts

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