Optimization, validation and application of headspace solid-phase microextraction gas chromatography for the determination of 1-nitro-2-phenylethane and methyleugenol from Aniba canelilla (H.B.K.) Mez essential oil in skin permeation samples

Kreutz, Tainá; Lucca, Letícia Grolli; Loureiro-Paes, Orlando A.R.; Teixeira, Hélder Ferreira; Veiga, Valdir Florêncio da; Limberger, Renata Pereira; Ortega, George González; Koester, Letícia Scherer

doi:10.1016/j.chroma.2018.05.073

Cited by 17 publications

(10 citation statements)

References 56 publications

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“…Polarity, molecular weight, and the size of the analyte of interest are the criteria that must be pondered before choosing the most suitable fiber for applying the HS-SPME technique. 27 Fibers coated with PDMS are generally better, as in the long term, they present stability and good performance during the processes of chromatography, extraction, and recovery of the analyte. Besides, these fibers are durable and usually hold satisfactory functioning for up to 100 analytical cycles.…”

Section: Results and Discussionmentioning

confidence: 99%

Headspace Solid-Phase Microextraction: Validation of the Method and Determination of Allyl Isothiocyanate Persistence in Cowpea Beans

et al. 2020

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Essential oils are widely recognized as an efficient and safe alternative for controlling pests in foods. However, a few studies have determined the persistence of these compounds in stored grains. The present study optimized and validated a fast and effective method for extraction and quantification of allyl isothiocyanate (AITC—the main component of mustard essential oil) residue in cowpea beans. It also investigated the persistence of this substance in the grains. The proposed method employs headspace solid-phase microextraction (HS-SPME) and gas chromatography with a flame ionization detector (GC/FID). For optimizing it, a central composite design (CCD) was used, where the best conditions for the extraction of the AITC were achieved using 15 min fiber exposure at 30 °C. The performance of the method was assessed by studying selectivity, linearity, limits of detection (LOD) and quantification (LOQ), precision, and accuracy. The LOD and LOQ for AITC were 0.11 and 0.33 μg kg –1 , respectively. The determination coefficient ( R 2 ) was above 0.99. The relative recovery rate ranged from 108.2 to 114.8%, with an interday coefficient of variation below 9%. After 36 h, no residue was detected in the samples, demonstrating that the AITC has low persistence and can be safely used as a bioinsecticide for grains.

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Section: Results and Discussionmentioning

confidence: 99%

Headspace Solid-Phase Microextraction: Validation of the Method and Determination of Allyl Isothiocyanate Persistence in Cowpea Beans

et al. 2020

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“…HS-SPME-GC/FID has advantages over other methods because it reduces or eliminates the need for organic solvents. Besides, it makes it possible to extract and concentrate the analyte simultaneously, thefore not requiring additional procedures for sample cleanup [42,43,51].…”

Section: Validation Of the Methods Of Extraction And Analysismentioning

confidence: 99%

“…Ultimately, the fiber of choice was the 50/30-µm StableFlex coated with DVB/CAR/PDMS, which delivered a response with a larger chromatographic area, indicating better safrole extraction. Polarity, molecular mass, and size of the analytes are criteria that also must be accounted for determining the most appropriate fiber for HS-SPME [42]. Xiao et al [45] assessed the performance of four fibers with different coatings for extracting volatile compounds from cherry wine-namely, 50/30-μm DVB/CAR/PDMS StableFlex, 65-μm PDMS/DVB, 75-μm CAR/PDMS, and 100-μm PDMS fibers.…”

Section: Choosing the Fibermentioning

confidence: 99%

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Method Validation and Evaluation of Safrole Persistence in Cowpea Beans Using Headspace Solid-Phase Microextraction and Gas Chromatography

et al. 2021

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Bioinsecticides are regarded as important alternatives for controlling agricultural pests. However, few studies have determined the persistence of these compounds in stored grains. This study aimed at optimizing and validating a fast and effective method for extraction and quantification of residues of safrole (the main component of Piper hispidinervum essential oil) in cowpea beans. It also sought to assess the persistence of this substance in the grains treated by contact and fumigation. The proposed method used headspace solid-phase microextraction (HS-SPME) and gas chromatography with a flame ionization detector (GC/FID). Factors such as temperature, extraction time and type of fiber were assessed to maximize the performance of the extraction technique. The performance of the method was appraised via the parameters selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy. The LOD and LOQ of safrole were 0.0057 and 0.019 μg kg−1, respectively and the determination coefficient (R2) was >0.99. The relative recovery ranged from 99.26 to 104.85, with a coefficient of variation <15%. The validated method was applied to assess the persistence of safrole residue in grains, where concentrations ranged from 1.095 to 0.052 µg kg−1 (contact) and from 2.16 to 0.12 µg kg −1 (fumigation). The levels measured up from the fifth day represented less than 1% of the initial concentration, proving that safrole have low persistence in cowpea beans, thus being safe for bioinsecticide use. Thus, this work is relevant not only for the extraction method developed, but also for the possible use of a natural insecticide in pest management in stored grains.

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“…However, high contents of methyleugenol were reported in the sapwood (65.3%) and heartwood (52.9%) tissues of A. canelilla . Additionally, significant contents of benzenoids, such as benzyl benzoate (78.1%) and phenylethyl benzoate (14.3%), were identified in the EO of A. gardineri ( Table 1 ) [ 12 , 27 , 83 , 84 , 85 , 86 , 87 ].…”

Section: Multivariate Statistical Analysis Based On the Essential Oils Of Aniba Speciesmentioning

confidence: 99%

Chemical Diversity and Therapeutic Effects of Essential Oils of Aniba Species from the Amazon: A Review

Trindade

Xavier

Setzer

et al. 2021

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Lauraceae families have great diversity in the world’s tropical regions and are represented mainly by aromatic shrubs and trees with significant production of essential oils (EOs). This work presents a review of the EO chemical profiles from specimens of Aniba, including their seasonal variations, geographical distributions, and biological activities in the Amazon biome. Based on the survey, 15 species were reviewed, representing 167 oil samples extracted from leaves, twig barks, and woods. Brazilian Amazon was the most representative geographic area in the number of specimens, highlighting the locations Belém, (Pará state, PA) (3 spp., 37 samples), Santarém (PA) (3 spp., 10 samples), Carajás (PA) (3 spp., 7 samples), and Manaus (Amazonas state, AM) (3 spp., 16 samples). The main compound classes identified in oils were benzenoids and phenylpropanoids, represented by 1-nitro-2-phenylethane, benzyl salicylate, benzyl benzoate and methyleugenol, along with terpenoids, especially monoterpenes and sesquiterpenes, such as linalool, α-phellandrene, β-phellandrene, β-selinene, and spathulenol. The EOs from Aniba showed considerable variation in the chemical profiles according to season and collection site. The hierarchical cluster analysis classified the samples into two main groups according to chemical composition. This review highlights its comprehensive and up-to-date information on history, conservation, traditional uses, chemosystematics, pharmacological potential of Aniba species.

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Optimization, validation and application of headspace solid-phase microextraction gas chromatography for the determination of 1-nitro-2-phenylethane and methyleugenol from Aniba canelilla (H.B.K.) Mez essential oil in skin permeation samples

Cited by 17 publications

References 56 publications

Headspace Solid-Phase Microextraction: Validation of the Method and Determination of Allyl Isothiocyanate Persistence in Cowpea Beans

Headspace Solid-Phase Microextraction: Validation of the Method and Determination of Allyl Isothiocyanate Persistence in Cowpea Beans

Method Validation and Evaluation of Safrole Persistence in Cowpea Beans Using Headspace Solid-Phase Microextraction and Gas Chromatography

Chemical Diversity and Therapeutic Effects of Essential Oils of Aniba Species from the Amazon: A Review

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