Optimization study of ethanolic fermentation from oil palm trunk, rubberwood and mixed hardwood hydrolysates using Saccharomyces cerevisiae

Abstract

Ethanolic fermentation using Saccharomyces cerevisiae was carried out on three types of hydrolysates produced from lignocelulosic biomass which are commonly found in Malaysia such as oil palm trunk, rubberwood and mixed hardwood. The effect of fermentation temperature and pH of hydrolysate was evaluated to optimize the fermentation efficiency which defined as maximum ethanol yield in minimum fermentation time. The fermentation process using different temperature of 25 degrees Celsius, 30 degrees Celsius and 40 degrees Celsius were performed on the prepared fermentation medium adjusted to pH 4, pH 6 and pH 7, respectively. Results showed that the fermentation time was significantly reduced with the increase of temperature but an adverse reduction in ethanol yield was observed using temperature of 40 degrees Celsius. As the pH of hydrolysate became more acidic, the ethanol yield increased. Optimum fermentation efficiency for ethanolic fermentation of lignocellulosic hydrolysates using S. cerevisiae can be obtained using 33.2 degrees Celsius and pH 5.3.

A Real Story of Bioethanol from Biomass: Malaysia Perspective

“…However, low-cost supply associated with high bioethanol yield of the bioethanol is indispensable for its wide use. The discussion of economic feasibility of bioethanol production from lignocellulosic biomass in Malaysia in this paper was based on the experimental data through laboratory worked done by [35] and [36] and comparison was made with sugarcane and corn.…”

“…As stated in the study by [35], optimum fermentation parameters for lignocellulosic hydrolysates using Saccharomyces cerevisae was obtained using 33.2°C and pH 5.3 with the fermentation efficiency of 80%, 85% and 90% for oil palm trunk, rubberwood and mixed hardwood, respectively. The optimum cellulose conversion and fermentation efficiency were used to calculate the actual ethanol yield per tonne (L/t) and the conversion efficiency of lignocellulosic biomass.…”

Bioenergy Production from Bamboo: Potential Source from Malaysia’s Perspective

“…In the process of bioethanol production, hydrolyzing of the lignocellulosic material to fermentable sugars faces a bottleneck situation due to the limitations set on the digestibility of lignocellulose by many physico-chemical, compositional, and structural factors (Chin et al 2010(Chin et al , 2011. Thus, pretreatment is a vital step in the production of fermentable sugars due to the recalcitrance characteristics of the feedstock.…”

Chemical Pretreatments of Wood Chips Prior to Alkaline Pulping - A Review of Pretreatment Alternatives, Chemical Aspects of the Resulting Liquors, and Pulping Outcomes

“…Production of fuel alcohols, such as ethanol or butanol from LCMs, has gained growing global attention during the past few decades (Brandberg et al 2004;Tang et al 2006;Margeot et al 2009;Chin et al 2010;Limayem and Ricke 2012;Sun and Liu 2012;Lee et al 2013). Depletion of fossil resources and growing demands for energy and chemicals have forced industries to search for alternative material sources.…”