Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment

Gamal, Amira A.; Abbas, Heba.Y.; Abdelwahed, Nayera A. M.; Kashef, Mona T.; Mahmoud, Khaled; Esawy, Mona A.; Ramadan, Mohamed A.

doi:10.1016/j.ijbiomac.2021.05.056

Cited by 22 publications

(9 citation statements)

References 36 publications

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“…Different concentrations of samples were produced, and in a U-shaped Ostwald viscometer, the flow time of equal volumes for each concentration at 30 °C was calculated ( Gamal et al, 2021 ). The flow time of the same volume of distilled water was also measured as a control.…”

Section: Methodsmentioning

confidence: 99%

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Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

et al. 2022

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Section: Methodsmentioning

confidence: 99%

“…The molecular weight was calculated using Mark-Houwink-Sakurada equation (Eq. (5) ) ( Gamal et al, 2021 ):

…”

Section: Methodsmentioning

confidence: 99%

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

et al. 2022

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“…The levan yielding organisms, Bacillus paranthracis (lev13M) [result under publication], Bacillus subtilis MT453867 (H) [9], Enterococcus faecalis Esawy (levG) [10], Pseudomonas aeruginosa HI1 from which the crude (levAE) and the dialyzed (levAE/D) levans were obtained [11] and Bacillus subtilis (9A) (lev9A) [result under publication] were cultivated on the medium containing (g/l): 80 sucrose, MgSO 4 0.2, KH 2 PO 4 1 and yeast extract 1, under shaking flask cultivation technique [12]. After the early stage of the stationary phase, the culture filtrate [CF] was centrifuged at 5000 ×g to get rid of bacterial cells.…”

Section: Levans' Precipitationmentioning

confidence: 99%

Possible correlation between probiotic activity of bacterial honey isolates and severe acute respiratory syndrome coronavirus 2 replication in vitro

et al. 2022

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Various strategies, like those using vaccines and antibiotics, have been examined for the prevention and treatment of virus's diseases, but until this moment infection control is not at sufficient level. Exopolysaccharides, especially from probiotics, became one of the most innovative approaches for antiviral agents. This research tried to highlight the effect of a probiotic polysaccharide, such as levan, in COVID-19 prevention. Accordingly, 5 levans types previously obtained from bacterial honey isolates were tested against COVID-19. The most promising result was recorded with levans from Pseudomonas aeruginosa HI1 (levAE) and Bacillus subtilis 9A (lev9A). The lowest cytotoxicity was obtained from lev9A (CC 50 =5.567e+006 mg/ml) and the most promising IC 50 was obtained by levAE (10.75 mg/ml) followed by lev13M (142.5 mg/ml) then lev9A (1299 mg/ml). The dialysis process of levAE greatly affected the virus inhibition activity (IC 50 of levAE/D =7.773e+006 mg/ml). Pseudomonas aeruginosa HI1 and Bacillus subtilis 9A were highly tolerant to the acidic (pHs 2, 3) and alkaline conditions (pHs 9, 11). Moreover, when incubated with 0.3 bile salt for 24h, their surviving rates recorded 94% and 100% respectively. H 2 O 2 tolerance showed 77% surviving of Pseudomonas aeruginosa HI1 and 100% surviving of Bacillus subtilis 9A. The blood hemolysis and the antibiotics sensitivity tests confirmed the isolate's safety. The hypothesis that the isolates adhere to the lung cells, could explain the ability of the isolates and their levans to inhibit covid-19 replication.

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“…For instance, FOS could be used as excellent prebiotic to support intestinal health owing to the non-digestible property . In addition, levan polymer can be applied as a stabilizer and texture modifier to improve food textural and sensory properties. , Moreover, some studies have reported that levan has potential pharmaceutical properties such as antipathogenic, anticancer, antiviral, and immunomodulatory activity, indicating it would be promising medicinal agent. − …”

Section: Introductionmentioning

confidence: 99%

Successful Manipulation of the Product Spectrum of the Erwinia amylovora Levansucrase by Modifying the Residues around loop1, Loop 3, and Loop 4

Zhang

et al. 2022

J. Agric. Food Chem.

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Levansucrase (LS, EC 2.4.1.10) catalyzes the synthesis of levan by successively transferring the fructosyl moiety from sucrose to an elongated fructan chain. Although the product distribution of LS from Erwinia amylovora (Ea-LS) was studied under different sucrose concentrations, the effect of residues on the product formation is yet unknown. The first levanhexaose-complexed structure of LS from Bacillus subtilis (Bs-SacB) provided information on the oligosaccharide binding sites (OB sites), from +1 to +4 subsites. Since Ea-LS would efficiently produce fructooligosaccharides, a substitution mutation of OB sites in Bs-SacB and the corresponding residues of Ea-LS were conducted to investigate how these mutants would influence the product distribution. As a result, a series of mutants with different product spectrum were obtained. Notably, the mutants of G98E, V151F, and N200T around loop 1, loop 3, and loop 4 all showed a significant increase in both the molecular mass and the yield of high-molecular-mass levan, suggesting that the product profile of Ea-LS was significantly modified.

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Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment

Cited by 22 publications

References 36 publications

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

Possible correlation between probiotic activity of bacterial honey isolates and severe acute respiratory syndrome coronavirus 2 replication in vitro

Successful Manipulation of the Product Spectrum of the Erwinia amylovora Levansucrase by Modifying the Residues around loop1, Loop 3, and Loop 4

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