Optimization Strategy for Isocratic Separation of Halogenated Phenols by Reversed Phase Liquid Chromatography with Methanol-Water Mobile Phase

Abstract

Halogenated phenols are priority pollutants. Their determination in drinking waters is, therefore, of great importance and many analytical methods have been developed. Chromatographic methods are suitable for the selective determination of individual phenolic compounds. The aim of this study is to develop a method for separating halogenated phenols such as 3-bromophenol, 3-chlorophenol, 4-fluorophenol, 4-chlorophenol and 4-iodophenol in complex matrices that both sensitive and selective. At the application of HPLC technique in the separation of halogenated phenols, the pH, concentration, in the presence of the water and temperature are important factors affecting its stability. The optimum mobile phase for the chromatographic separation was found to be methanol-water (50:50 v/v) with 5 × 10 -3 M hexanesulfonic acid, pH of 4.552 and optimum heat 25 ºC.