Optimization of α-Terpineol Production by the Biotransformation of R-(+)-Limonene and (−)-β-Pinene

Abstract

The main objective of this work is to present the optimization of the biotransformation of R-(+)-limonene and (-)-β-pinene aiming at the production of α-terpineol by strains of fungal and yeasts previously isolated by our research group using the methodology of experimental design. New optimized experimental data on α-terpineol production by the biotransformation of R-(+)-limonene and (-)-β-pinene using newly isolated microorganisms are reported in this work. Conversion of about 1,700 mg/L was achieved when R-(+)-limonene was used as substrate and the newly isolated strain 05.01.35 as microorganism at the central point of the experimental design, corresponding to a substrate concentration of 1.75%, mass of inoculum of 2 g, and substrate to ethanol volume ratio of 1:1. The same experimental condition led to higher conversions when (-)-β-pinene was used as substrates and the strains coded as 04.05.08 and 01.04.03 as microorganism. Here, conversions of about 770 mg/L were achieved.

Fungal biotransformation of limonene and pinene as a biotechnological approach for production of aroma compounds

“…Under these conditions, the concentration of α-terpineol in the culture medium was reached using Aspergillus sp. (04.05.08) and (01.04.03), and higher production was also obtained in the central point of the experimental design, about 761 mg/L and 763 mg/L, respectively (Rottava et al 2011b).…”

Optimization of Culture Conditions for the Efficient Biosynthesis of Trilobatin from Phloretin by Engineered Escherichia coli Harboring the Apple Phloretin-4′-O-glycosyltransferase

“…The BL21 (DE3)­pLysS cells also recorded a higher phloretin concentration (41.95 μM) as well as a higher trilobatin/phloretin ratio (Figure ). Yield and conversion efficiency are affected by substrate concentration; − therefore, the effect of phloretin concentrations on trilobatin production was examined for the BL21 (DE3)­pLysS strain by feeding with different concentrations of phloretin. These cells produced 20.99 μM (9.12 mg L –1 ) trilobatin after feeding with 250 μM phloretin (Figure ).…”

Processos de bioconversão aplicados à obtenção de fármacos e intermediários

“…Avaliando-se estatisticamente as respostas pré--selecionadas para traduzir a eficiência do processo, é possível aplicarem-se várias etapas sequenciais de planejamento experimental. Observando-se as tendências assumidas pelo processo a partir da influência de cada variável e das possíveis combinações entre elas, pode-se refinar paulatinamente os resultados em direção à otimização do processo [59][60][61][62] .…”