Optimization of Xylanase Production from Aspergillus Flavus in Solid State Fermentation Using Agricultural Waste as a Substrate

Abstract

In the present study an attempt was made to optimize the cultural conditions using agricultural substrate for xylanase production by Aspergillus flavus in solid state fermentation process. The optimization of cultural conditions such as optimal temperature, pH and inoculam size was studied and best choice for agricultural substrate among wheat bran, wheat bran, and soya bran substrates in solid state fermentation for xylanase production has been investigated. Among three different agricultural substrates rice bran shows the maximum enzyme activity of 162.0±3.0 unit /ml. The enzyme assay was carried out at different temperatures and pH which shows maximum enzyme activity of 172.0±4.1 units /ml in wheat bran at 32 0 C and pH 8 produce the maximum enzyme activity in soya bran 183.0±5.1 units /ml respectively. Optimization of inoculum size for xylanase production shows 140 spores/ ml that is 184.0±2.8 units /ml in wheat bran used as the substrate.With this studies the optimized culture medium for the production of the enzyme and to get the maximum enzymatic activity with the optimized temperature32°C, pH 8, substrate wheat bran and the inoculmn size of 140 spores/ml shows the maximum enzyme activity of 190±3.1units /ml.