Optimization of variable fluorescence measurements of phytoplankton communities with cyanobacteria

Simis, Stefan; Huot, Yannick; Babin, Marcel; Seppälä, Jukka; Metsamaa, Liisa

doi:10.1007/s11120-012-9729-6

Cited by 74 publications

(69 citation statements)

References 43 publications

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“…We chose 455 nm as a compromise excitation wavelength absorbed by the long side of the chlorophyll a (or divinyl chlorophyll a) absorbance band, which is the only pigment present across all our diverse study strains. Emerging multi-spectra chlorophyll fluorometers will support more generalized analyses across multiple excitation bands delivered through different antenna complexes (Simis et al, 2012) across taxa.…”

Section: Methodsmentioning

confidence: 99%

Phytoplankton σPSII and Excitation Dissipation; Implications for Estimates of Primary Productivity

Lavaud

Perkins

et al. 2018

Front. Mar. Sci.

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The effective absorption cross section for photochemistry of Photosystem II in the light (σ PSII ′ ) comprises the probability of light capture by Photosystem II and the quantum yield for subsequent photochemistry. σ PSII ′ is used to model photosynthesis and aquatic productivity, and phytoplankters regulate σ PSII ′ to mitigate over-or under-excitation of Photosystem II. We used diverse phytoplankton taxa to compare short and long term changes in σ PSII ′ with the induction of the yield of non-photochemical quenching (YNPQ) of chlorophyll fluorescence, a measure of regulated excitation dissipation. In two picocyanobacteria σ PSII ′ showed no decline upon induction of moderate YNPQ, above light levels sufficient for saturation of electron transport. In the eukaryotic chl a/b Ostreococcus and the chl a/c diatom Thalassiosira, induction of non-photochemical quenching was stronger after growth under saturating light, an acclimation attributable to increased xanthophyll cycle pigment content. Across short and longer-term light histories to induce or relax regulatory processes Ostreococcus and Thalassiosira showed proportional variations between the level of YNPQ and the down regulation of σ PSII ′ . The proportional down regulation of σ PSII ′ was, however, significantly smaller than the amplitude of YNPQ induction. For the eukaryotes we can predict changes in σ PSII ′ , useful for modeling electron transport, productivity and acclimation, from measures of YNPQ, which are accessible from fluorescence yield measures that do not include σ PSII ′ . This useful relation, however, does not extend to the tested prokaryotes, possibly as a result of differential violations of the rate constant assumptions that underlie the calculated YNPQ parameter.

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Section: Methodsmentioning

confidence: 99%

Phytoplankton σPSII and Excitation Dissipation; Implications for Estimates of Primary Productivity

Lavaud

Perkins

et al. 2018

Front. Mar. Sci.

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“…Moreover, owing to the differences in light harvesting systems (pigment-protein binding in particular), especially in dinoflagellates and cyanobacteria (see MacIntyre et al, 2010;Millie, Schofield, Kirkpatrick, Johnsen, & Evens, 2002), the peak wavelength of fluorescence depends also on the species composition of phytoplankton. Thus, for example, phycocyanin-rich cyanobacteria have an additional emission peak at~655 nm due to phycocyanin (MacIntyre et al, 2010;Simis, Huot, Babin, Seppälä, & Metsamaa, 2012), which is close to the first chosen baseline for the MODIS algorithm (667 nm). Therefore, in the case of multispectral retrievals the position of spectral bands for the nFLH retrieval can lead to a smaller sensitivity and lower fluorescence values.…”

Section: Comparison With Modis Nflh Datamentioning

confidence: 75%

Global retrieval of marine and terrestrial chlorophyll fluorescence at its red peak using hyperspectral top of atmosphere radiance measurements: Feasibility study and first results

Wolanin

Розанов

Dinter

et al. 2015

Remote Sensing of Environment

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Keywords:Sun-induced fluorescence Retrieval SCIAMACHY GOME-2 Fraunhofer-line approach Hyperspectral remote sensing Ocean-atmosphere coupled radiative transfer Chlorophyll fluorescence is directly linked to the physiology of phytoplankton or plants. Here, we present a new satellite remote sensing approach to retrieve chlorophyll fluorescence at its red peak (~685 nm) by using measurements from the hyperspectral instruments SCanning Imaging Absorption SpectroMeter for Atmospheric CHartographY (SCIAMACHY) and Global Ozone Monitoring Experiment-2 (GOME-2). This method, which is based on the Differential Optical Absorption Spectroscopy (DOAS) technique, was used to exploit narrow spectral structures resulting from the filling-in of the Fraunhofer Fe I line, which originates from fluorescence. The reference spectra for chlorophyll fluorescence were calculated by the coupled ocean-atmosphere radiative transfer model SCIATRAN. We compared our results on marine chlorophyll fluorescence observations with the MODIS Terra normalized Fluorescence Line Height (nFLH) product for the average of years 2003-2011 and year 2009. Our method also enables the retrieval of chlorophyll fluorescence above land vegetation scenes. The results for the fluorescence observed above terrestrial vegetation for July and December 2009 were compared to MODIS Enhanced Vegetation Index (EVI). The comparisons show good spatial agreement between different retrievals providing evidence for the good performance of our algorithm. The method presented is generic and can be applied to other hyperspectral instruments in the future. Having established the retrieval technique, extensive studies of chlorophyll fluorescence will improve global knowledge on physiology and photosynthetic efficiency, in both the marine and terrestrial realms, and its dependence on environmental factors.

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“…However, it may be that some of the phycobilin pigments tagged along with the lipids during the extraction as in the case of Nodularia harveyana. Simis et al (2012) showed that phycocyanin, which might be mixed with phycoerythrocyanin, is the most abundant photosynthetic accessory pigment. Apart from the chlorophyll pigments, Leptolyngbya spp.…”

Section: Lipid Extractsmentioning

confidence: 99%

An investigation of biodiesel production from microalgae found in Mauritian waters

Beetul¹,

Sadally²,

Taleb-Hossenkhan³

et al. 2014

Biofuel Res. J.

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HIGHLIGHTSTotal micro-phytoplankton count amounted to 6.59±1.27x10 5 cells L -1 which was dominated by diatoms (95.2%), followed by dinoflagellates (2.9%) and cyanobacteria (1.9%).  The cyanobacterial mats were identified as Leptolyngbya sp. and Nodularia harveyana, and the endosymbiotic dinoflagellates as the Symbiodinium clade C.  There were also differences recorded in the % lipid of the different microalgae (p<0.005) -among all, Symbiodinium clade C had the highest with (38.39±6.58%). The aim of this study was to assess the lipid content and the subsequent potential of different microalgae present in the Mauritian marine water to produce biodiesel. The share of micro-phytoplankton species in the water column was determined. The cyanobacterial mats and endosymbiotic dinoflagellates were characterised morphologically and genetically using RFLP. The samples were quantified gravimetrically and analysed using 1 H & 13 C NMR spectroscopy. Total micro-phytoplankton count amounted to 6.59±1.27x10 5 cells L -1 which was dominated by diatoms (95.2%), followed by dinoflagellates (2.9%) and cyanobacteria (1.9%). The cyanobacterial mats were identified as Leptolyngbya sp. and Nodularia harveyana, and the RFLP characterised the endosymbiotic dinoflagellates as the Symbiodinium clade C. The highest amount of lipid was recorded in the Symbiodinium clade C (38.39±6.58%).1 H and 13 C NMR analyses indicated the presence of acyl glycerols. An attempt to synthesise biodiesel by alkaline trans-esterification reaction was also performed and the presence of biodiesel was detected using the Fourier Transform Infrared Spectroscopy. The Infrared analysis yielded peaks at around 1738cm -1 and 1200cm -1 characteristic of the carbonyl and ether groups respectively, indicating the presence of biodiesel.

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Optimization of variable fluorescence measurements of phytoplankton communities with cyanobacteria

Cited by 74 publications

References 43 publications

Phytoplankton σPSII and Excitation Dissipation; Implications for Estimates of Primary Productivity

Phytoplankton σPSII and Excitation Dissipation; Implications for Estimates of Primary Productivity

Global retrieval of marine and terrestrial chlorophyll fluorescence at its red peak using hyperspectral top of atmosphere radiance measurements: Feasibility study and first results

An investigation of biodiesel production from microalgae found in Mauritian waters

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