Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo

Tian, Chunlian; Chang, Yu Chia; Wang, Ruxia; Kang, Zhiruo; Wang, Qi; Tong, Zhi-Fan; Zhou, Aohua; Cui, Cai-Lian; Liu, Mingchun

doi:10.1016/j.jep.2021.114225

Cited by 12 publications

(8 citation statements)

References 47 publications

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“…The content of total flavonoids in quinoa was determined with rutin as the standard, and the absorbance was measured at 500 nm using a UV‐visible spectrophotometer. The total polysaccharide content of quinoa was determined by the phenol‐sulfuric acid method with glucose as the standard, and the absorbance was measured at 490 nm 18‐20 …”

Section: Methodsmentioning

confidence: 99%

“…The total polysaccharide content of quinoa was determined by the phenol-sulfuric acid method with glucose as the standard, and the absorbance was measured at 490 nm. [18][19][20] Ultra-high performance liquid chromatographyquadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) conditions for quinoa analysis Quinoa flour was made into a solution of 100 mg L −1 with deionized water, and the supernatant was filtered through a 0.22-mm microporous membrane. All samples were analyzed by UHPLC-Q-TOF-MS high-resolution four-stage flight tandem liquid chromatography-mass spectrometry (Xevo G2-XS Qtof; Waters Corp. Milford, MA, USA) for positive and negative ion mode analysis.…”

Section: Determination Of Total Saponins Total Flavonoids and Total P...mentioning

confidence: 99%

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The protective effect of quinoa on the gastric mucosal injury induced by absolute ethanol

et al. 2022

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BACKGROUND: Gastric mucosal injury caused by ethanol is a common gastrointestinal disease. Quinoa (Chenopodium quinoa Willd.), as a nutrient-rich grain, plays a significant role in preventing and treating gastric mucosal damage. The present study aimed to explore the protective effect of quinoa on alcohol-induced gastric mucosal damage and its possible mechanism.RESULTS: The ethanol-induced gastric mucosal injury rat model was used for in vivo experiments and H 2 O 2 -induced GES-1 cells for in vitro experiments to elucidate the protective effect of quinoa. The results show that quinoa water extract can increase the superoxide dismutase level and decrease the malondialdehyde level in vitro and in vivo. Furthermore, quinoa also reduced the bleeding point and bleeding area in rats with ethanol-induced gastric mucosal injury and improved gastric histopathological changes. H 2 O 2 significantly increased the levels of inflammatory factors in GES-1 cells, which were markedly ameliorated by quinoa water extract. Likewise, quinoa water extract regulated the protein expression levels of Nrf2, Keap1, HO-1, p-IKK, and p-NF-κB through Nrf2 and nuclear factor-κB signaling pathways, reducing the production of oxidative stress and inflammation, thereby repairing the damaged gastric mucosa. CONCLUSION:The findings of this study demonstrated that quinoa shows protective effect against ethanol-induced gastric mucosal injury through its anti-inflammatory and anti-oxidant effects. We propose that our research will provide a reference for quinoa as a functional food.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of Total Saponins Total Flavonoids and Total P...mentioning

confidence: 99%

The protective effect of quinoa on the gastric mucosal injury induced by absolute ethanol

et al. 2022

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“…Recently, many epidemiological and experimental studies have shown saponins’ beneficial effects on human health. Several sources of evidence suggest that saponins not only seem to have antioxidant and cholesterol-lowering capacities, but also anti-inflammatory, antibacterial, antidiabetic, and neuro-protective activities, as well as stereoselective effects on ion channel current regulation and the cardiovascular system, immunostimulation, and protection against the risk of cancer development [ 39 , 40 , 103 , 104 ].…”

Section: Health Benefits Of Saponinsmentioning

confidence: 99%

“…Saponins from lentils were also studied as anti-inflammatory agents [ 104 ]. Conti et al (2021) investigated the impact of a legume diet on elderly people and underlined how soyasaponins from lentils and other legumes can exhibit anti-inflammatory activity.…”

Section: Health Benefits Of Saponinsmentioning

confidence: 99%

Polyphenols, Saponins and Phytosterols in Lentils and Their Health Benefits: An Overview

et al. 2022

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The lentil (Lens culinaris L.) is one of the most important legumes (family, Fabaceae). It is a natural functional food rich in many bioactive compounds, such as polyphenols, saponins and phytosterols. Several studies have demonstrated that the consumption of lentils is potentially important in reducing the incidence of a number of chronic diseases, due to their bioactive compounds. The most common polyphenols in lentils include phenolic acids, flavan-3-ol, flavonols, anthocyanidins, proanthocyanidins or condensed tannins, and anthocyanins, which play an important role in the prevention of several degenerative diseases in humans, due to their antioxidant activity. Furthermore, lentil polyphenols are reported to have antidiabetic, cardioprotective and anticancer activities. Lentil saponins are triterpene glycosides, mainly soyasaponins I and βg. These saponins have a plasma cholesterol-lowering effect in humans and are important in reducing the risk of many chronic diseases. Moreover, high levels of phytosterols have been reported in lentils, especially in the seed coat, and β-sitosterol, campesterol, and stigmasterol are the most abundant. Beyond their hypocholesterolemic effect, phytosterols in lentils are known for their anti-inflammatory activity. In this review, the current information on the nutritional composition, bioactive compounds including polyphenols, saponins and phytosterols, and their associated health-promoting effects are discussed.

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“…To date, many kinds of chromatographic approaches, such as UPLC [13][14][15][16][17], HPLC [18][19][20], SFC [21,22], TLC [23,24], and GC [25,26] have been applied for the measurement of pharmaceuticals in various matrixes. Among them, HPLC is the most potent approach [27].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of ginsenosides imprinted polymer‐based chromatographic stationary phase

Xue

Zhang

et al. 2023

J of Separation Science

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The molecular imprinting technique has aroused great interest in preparing novel stationary phases, and the resulting materials named molecularly imprinted polymers coated silica packing materials exhibit good performance in separating diverse analytes based on their good characteristics (including high selectivity, simple synthesis, and good chemical stability). To date, monotemplate is commonly used in synthesizing molecularly imprinted polymersbased stationary phases. The resulting materials always own the disadvantages of low column efficiency and restricted analytes, and the price of ginsenosides with high purity was very high. In this study, to overcome the weaknesses of molecularly imprinted polymers-based stationary phases mentioned above, the multi-templates (total saponins of folium ginseng) strategy was used to prepare ginsenosides imprinted polymer-based stationary phase. The resulting ginsenosides imprinted polymer-coated silica stationary phase has a good spherical shape and suitable pore structures. Additionally, the total saponins of folium ginseng were cheaper than other kinds of ginsenosides. Moreover, the ginsenosides imprinted polymer-coated silica stationary phase-packed column performed well in the separation of ginsenosides, nucleosides, and sulfonamides. The ginsenosides imprinted polymer-coated silica stationary phase possesses good reproducibility, repeatability, and stability for seven days. Therefore, a multitemplates strategy for synthesizing the ginsenosides imprinted polymer-coated silica stationary phase is considered in the future. K E Y W O R D Schromatographic properties, molecular imprinting, polar pharmaceuticals, stationary phases, templates Article Related Abbreviations: EtOH, ethanol; HAC, acetic acid; MeOH, methanol; MIPs/SiO 2 , molecularly imprinted polymers coated silica; NIP/SiO 2 , non-imprinted polymer-modified silica microspheres; Sil-PEI/CDs, polyethyleneimine-functionalized carbon dots fabricated silica microbeads; XPS, X-ray photoelectron spectroscopy; γ-MPS, 3-(trimethoxysilyl)propyl methacrylate.

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Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo

Cited by 12 publications

References 47 publications

The protective effect of quinoa on the gastric mucosal injury induced by absolute ethanol

The protective effect of quinoa on the gastric mucosal injury induced by absolute ethanol

Polyphenols, Saponins and Phytosterols in Lentils and Their Health Benefits: An Overview

Synthesis and evaluation of ginsenosides imprinted polymer‐based chromatographic stationary phase

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