Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Abstract

In this study, response surface methodology was employed to optimize the ultrasound‐assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP). The optimal conditions for UAE of PSMP were: liquid–solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%. The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography‐mass spectrometry, Fourier‐transfer infrared spectroscopy, and nuclear magnetic resonance detections. Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE. The antioxidant activities of PSMP were investigated using various assays in vitro. The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods. Practical Application The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources. With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays. This study provides effective reference for in‐depth research on other medicine‐food dual‐use resources. Ultrasound‐assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving. In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.

Critical Variables Influencing the Ultrasound-Assisted Extraction of Bioactive Compounds—A Review

“…However, despite the use of methanol tends to obtain better extraction yields, ethanol is preferably chosen because of its lower toxicity [10]. For example, distilled water and ethanol are usually used for pigments extraction, while water is the most common solvent for the extraction of polysaccharides and amino acids [12,20,21] (Table 1). Furthermore, European Directive 2010/59/EU lists the solvents that can be used for the extraction of compounds from foodstuffs, as well as their uses and limitations [22].…”

Optimization of complex enzyme‐ultrasonic synergistic extraction of water‐soluble polysaccharides from Perilla frutescens seed meal: Purification, characterization and in vitro antioxidant activity

“…DPPH radical scavenging activity of PSMP‐1 was determined according to our previous method (Zhang et al., 2021). Briefly, a 60 μmol/L ethanol‐free DPPH radical solution was prepared.…”

Optimization of Flavonoid Extraction from Xanthoceras sorbifolia Bunge Flowers, and the Antioxidant and Antibacterial Capacity of the Extract

“…On the basis of single factor experimental results, the extraction of TFs from X. sorbifolia Bunge flower was optimized using the response surface methodology (RSM) [ 22 , 23 ]. In this study, the Box–Behnken design method provided by Design Expert 11 (Stat-Ease Inc., Minneapolis, MN, USA) was selected to guide the experimental design with four variables (extraction temperature, ethanol concentration, solid–liquid ratio, and extraction time) at three levels, whose response value was the extraction yield of the TFs (Y) [ 24 ]. The Box–Behnken design method with four factors and three levels consisted of 29 randomized experiments [ 25 ].…”