Optimization of ultrasound assisted extraction of anthocyanins from red cabbage using Taguchi design method

Ravanfar, Raheleh; Tamadon, Ali Mohammad; Niakousari, Mehrdad

doi:10.1007/s13197-015-1880-6

Cited by 47 publications

(31 citation statements)

References 28 publications

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“…Nas et al (2005) showed that methods based on fractional factorial designs, such as Plackett-Burman designs (PBD) or fractional factorial design (FFD), can be used in plant tissue culture experiments and significantly reduce the number of optimisation experiments compared with that needed for SPD, CRD and RCBD. The Taguchi method utilises the same concept with two-, three-and mixed-level fractional factorial designs, which renders this a robust method for optimisation of industrial processes (Rao et al 2008;Ravanfar et al 2015). The Taguchi method has been applied successfully in a range of biological experiments (Orłowska et al 2020;Ramakrishna et al 2013;Ravanfar et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The Taguchi method utilises the same concept with two-, three-and mixed-level fractional factorial designs, which renders this a robust method for optimisation of industrial processes (Rao et al 2008;Ravanfar et al 2015). The Taguchi method has been applied successfully in a range of biological experiments (Orłowska et al 2020;Ramakrishna et al 2013;Ravanfar et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Orłowska

Bednarek

2020

Plant Mol Biol

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Key message The Taguchi method and metAFLP analysis were used to optimise barley regenerants towards maximum andminimum levels of tissue culture-induced variation. The subtle effects of symmetric and asymmetric methylationchanges in regenerants were identified. Abstract Plant tissue cultures (PTCs) provide researchers with unique materials that accelerate the development of new breeding cultivars and facilitate studies on off-type regenerants. The emerging variability of regenerants derived from PTCs may have both genetic and epigenetic origins, and may be desirable or degrade the value of regenerated plants. Thus, it is crucial to determine how the PTC variation level can be controlled. The easiest way to manipulate total tissue cultureinduced variation (TTCIV) is to utilise appropriate stress factors and suitable medium components. This study describes the optimisation of in vitro tissue culture-induced variation in plant regenerants derived from barley anther culture, and maximizes and minimizes regenerant variation compared with the source explants. The approach relied on methylation amplified fragment length polymorphism (metAFLP)-derived TTCIV characteristics, which were evaluated in regenerants derived under distinct tissue culture conditions and analysed via Taguchi statistics. The factors that may trigger TTCIV included CuSO 4 , AgNO 3 and the total time spent on the induction medium. The donor plants prepared for regeneration purposes had 5.75% and 2.01% polymorphic metAFLP loci with methylation and sequence changes, respectively. The level of TTCIV (as the sum of all metAFLP characteristics analyzed) identified in optimisation and verification experiments reached 7.51 and 10.46%, respectively. In the trial designed to produce a minimum number of differences between donor and regenerant plants, CuSO 4 and AgNO 3 were more crucial than time, which was not a significant factor. In the trial designed to produce a maximum number of differences between donor and regenerant plants, all factors had comparable impact on variation. The Taguchi method reduced the time required for experimental trials compared with a grid method and suggested that medium modifications were required to control regenerant variation. Finally, the effects of symmetric and asymmetric methylation changes on regenerants were identified using novel aspects of the metAFLP method developed for this analysis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Orłowska

Bednarek

2020

Plant Mol Biol

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“…Suitable power with lower frequencies is better than higher frequencies in extraction yield. Ravanfar et al (2015) reported when high power ultrasonic is applied, the sound energy is converted into heat causing the anthocyanin degradation. Therefore exposing the materials containing anthocyanin which is very sensitive to temperature ultimately lead to low anthocyanin yield (Chigurupati et al, 2002).…”

Section: Total Anthocyanin Contents (Tac)mentioning

confidence: 99%

Comparison of hot water and methanol extraction combined with ultrasonic pretreatment on antioxidant properties of two pigmented rice cultivars

Tobgay¹,

Boonyanuphong²,

Meunprasertdee³

2019

Food Res.

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Pigmented rice is considered as the good source of phytochemicals which contains more phenolic contents and higher antioxidant activities compared with non-pigmented rice. However, those phytochemicals are normally extracted using inorganic solvent, using water was rarely found. This study was aimed to compare extraction methods on antioxidant contents and activities of two types of Thai pigmented rice (black glutinous rice and red non-glutinous rice). Pigmented rice was extracted either conventional method alone or a combination of ultrasonic plus conventional method with different solvents (methanol and hot water). Phenolic contents were analyzed by spectrophotometric assay and high-performance liquid chromatography (HPLC). Antioxidant activities were investigated by radical scavenging capacity (ABTS and DPPH). The results showed that ultrasonic pretreatment significantly enhanced the total phenolic, flavonoid, anthocyanin contents and higher antioxidant activities compared with conventional extraction irrespective of solvents and rice varieties. Ultrasonic methanol (UM) extraction proved to extract significant higher content of all the compounds analyzed in both the rice varieties. In contrast, hot water (HW) extracts exhibited the lowest amount of bioactive compounds. However, there was no significant difference between methanol extraction alone and ultrasonic pretreatment with hot water extract on antioxidant contents for black glutinous rice. The contents of individual anthocyanins and flavonoids (cyanidin 3-O-glucoside, peonidin 3-O-glucoside, and quercetin) were significantly increased with ultrasonic pretreatment compare to the conventional method in black glutinous rice while failed to detect the red rice variety. The black glutinous rice contained significantly higher contents of all analyzed compounds and antioxidant activities than red rice. In conclusions, our results demonstrated that ultrasonic pretreatment significantly enhanced antioxidant extraction with higher activities compared with conventional extraction irrespective of solvents and rice varieties. Furthermore, the efficiency of ultrasonic together with hot water extraction was almost equal to methanol extraction which was the one effective solvent for extracting antioxidant.

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“…They suggest that their approach allows a potential energy savings as much as 48% according to conventional approaches. There are more studies about the extractions, Taguchi methods, and the heat and mass transfers in the literature [12], [15]- [18].…”

Section: Introductionmentioning

confidence: 99%

An Experimental Study on Extraction of Sugar from Carob Using with Taguchi Method

Durmaz¹,

Ozel²

2019

Sakarya University Journal of Science

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The carob, a perennial plant, is grown in regions where the Mediterranean climate is dominant. Because of its properties, it is preferred in many sectors especially in food and health sectors. Carob is mostly used as sugar and molasses. Moreover, seeds of carob are also used as thickening material in the food sector and its residue is utilized as animal feeding stuff as well. Molasses are mostly produced from carob, grape, mulberry, apple and pear for many years in Turkey. Molasses production is different from each other with regard to the structure of each fruit. Molasses are produced by pressing in the event of soft. The molasses of hard dried fruits such as carobs which cannot be pressed are obtained by extraction method using water. The parameters which are important for the quality of product such as extraction temperature and duration are not taken into account in the carob molasses obtained by traditional methods. Increasing the amount of water in extraction decreases production efficiency and quality while increasing energy consumption. In this study, the effects of parameters such as water quantity, extraction temperature, duration and batch number that affect quality of molasses were experimentally investigated by using Taguchi method.

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Optimization of ultrasound assisted extraction of anthocyanins from red cabbage using Taguchi design method

Cited by 47 publications

References 28 publications

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Comparison of hot water and methanol extraction combined with ultrasonic pretreatment on antioxidant properties of two pigmented rice cultivars

An Experimental Study on Extraction of Sugar from Carob Using with Taguchi Method

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