Optimization of ultrasound-assisted cellulase degradation method on the extraction of mulberry leaf protein and its effect on the functional characteristics

Wang, Fan; Ren, Xiaolan; Duan, Hanyi; Guo, Xiaojun; Zhang, Yachao; Li, Jisheng; Zhang, Fengying; Chen, J. Y.

doi:10.1016/j.ultsonch.2023.106561

Cited by 3 publications

(1 citation statement)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation aligned well with previous investigations on protein extraction from wheat flour and rice bran, wherein most protein was extracted within the initial 60 s of sonication (Morel et al, 2000;Ly et al, 2018;Singh and MacRitchie, 2001). However, a reported sonication time of 10-60 min was found to facilitate the highest protein yield from M. oleifera leaves (Cheng et al, 2021), mulberry leaves (Fan et al, 2023;Zhao et al, 2023), and Diplazium esculentum leaves (Dai et al, 2020). This discrepancy could be due to the sample preparation and pre-treatment in which the leaf meals with particle size of no greater than 177 µm were employed and softened before sonication.…”

Section: Effects Of Sonication Timesupporting

confidence: 89%

Optimisation of Protein Extraction from Selenium-Enriched Brassicaceae Leaves

Rawiwan,

Xiang,

Quek

2023

Preprint

View full text Add to dashboard Cite

Section: Effects Of Sonication Timesupporting

confidence: 89%

Optimisation of Protein Extraction from Selenium-Enriched Brassicaceae Leaves

Rawiwan,

Xiang,

Quek

2023

Preprint

View full text Add to dashboard Cite

Optimisation of protein extraction from selenium-enriched Brassicaceae leaves

Rawiwan,

Xiang,

Quek

2023

Future Foods

View full text Add to dashboard Cite

Isolation, Characterization, and Functional Properties of Antioxidant Peptides from Mulberry Leaf Enzymatic Hydrolysates

Zhou,

Zhang,

Wang

et al. 2024

Antioxidants

View full text Add to dashboard Cite

Recent evidence suggests that mulberry leaves have good antioxidant activity. However, what the antioxidant ingredient is and how the ingredient works are still not well understood. In this study, we enzymatically hydrolyze mulberry leaf proteins (MLPs) using neutral protease and find that the mulberry leaf protein hydrolysates (MLPHs) have stronger antioxidant activity compared to MLPs. We separate the core antioxidant components in MLPHs by ion-exchange columns and molecular sieves and identify 798 antioxidant peptides by LC-MS/MS. Through bioinformatics analysis and biochemical assays, we screen two previously unreported peptides, P6 and P7, with excellent antioxidant activities. P6 and P7 not only significantly reduce ROS in cells but also improve the activities of the antioxidant enzymes SOD and CAT. In addition, both peptides are found to exert protective effects against H2O2-induced chromatin damage and cell apoptosis. Collectively, these results provide support for the application of mulberry leaf peptides as antioxidants in the medical, food and livestock industries.

show abstract

Optimization of ultrasound-assisted cellulase degradation method on the extraction of mulberry leaf protein and its effect on the functional characteristics

Cited by 3 publications

References 38 publications

Optimisation of Protein Extraction from Selenium-Enriched Brassicaceae Leaves

Optimisation of Protein Extraction from Selenium-Enriched Brassicaceae Leaves

Optimisation of protein extraction from selenium-enriched Brassicaceae leaves

Isolation, Characterization, and Functional Properties of Antioxidant Peptides from Mulberry Leaf Enzymatic Hydrolysates

Contact Info

Product

Resources

About