Optimization of ultrasonic-assisted extraction of polyphenols from the polyherbal formulation of Cinnamomum verum, Origanum majorana, and Origanum vulgare and their anti-diabetic capacity in zebrafish (Danio rerio)

Abstract

The Cinnamomum verum (CV) , Origanum majorana (CM), and Origanum vulgare (OV) have been used in traditional medicine in several regions of México for their anti-diabetic properties. In this study investigated the variables of ultrasound-assisted extraction for the polyphenolic compounds from the combination of these plants and explore their potential antidiabetic activities on glucose-induced-diabetic zebrafish. Determined the optimum conditions for ultrasonic-assisted extraction (UAE) to maximum recovery amounts of phenolic compounds from the extract of these plants. Polyphenols were detected in the extracts using HPLC-DAD-analysis. Extracts were evaluated on zebrafish exposed to high glucose concentration (110 mM) for two weeks. Results showed second-order polynomial mathematical models with a high coefficient of determination (R2 > 0.9564). Optimized extraction conditions for UAE from the combination of the 3 plants (COV) were as follows: 66.03%, ethanol, 28.87 min, and 21.51 mL/g for maximal flavonoids extraction. Used the same optimal extraction conditions for CV, CM, and OV. Results from LC-MS/MS indicated 9 polyphenolic compounds in CV, 12 in CM, and 6 in OV, the content of total polyphenols was 310.28, 90.42, and 126.74 mg GAE 100 g −1 dry weight, respectively. However, hyperglycemic fish showed an increase in cholesterol and triglyceride levels whereas extracts completely prevented these metabolic alterations. COV showed higher anti-diabetic ability than CV, CM, and OV, suggesting a synergistic effect between them. Our investigation developed a new herbal formulation of Cinnamomum verum; Origanum majorana; Origanum vulgare that has proven effective in animals with type 2 diabetes will form a new class of supplements to treat diabetic complications.

Assessing the Ameliorative Effect of Selenium Cinnamomum verum, Origanum majorana, and Origanum vulgare Nanoparticles in Diabetic Zebrafish (Danio rerio)

“…Ten grams of polyherbal formulation powder was placed in a capped tube and subjected to UAE using an ultrasonic cleaning bath (KH5200 DB type, Kunshan ultrasonic instrument Co., Ltd., Kunshan, China). The process was carried out using a frequency of 40 kHz and at 200 W with the following optimal extraction conditions: ethanol 66.03%, 28.87 min, and 21.51 mL/g for maximal flavonoids extraction [ 49 ]. After we collected the supernatant using UAE, the samples were centrifuged at 5000 rpm for 10 min and UV–Vis analyses were performed at 360 nm.…”

The Relevance of Some Plant Extracts In Human Patients and Animal Models of Diabetes

“…Blood glucose, cholesterol and triglyceride levels were reduced in diabetic zebrafish induced by exposure to a 110 mM concentration of glucose following exposure to the extract of Cinnamomum verum, Origanum majorana and Origanum vulgare, while exposure to the polyherbs formed from these three extracts together induced a significant reduction in hyperglycemia and hyperlipidemia [19].…”

Ultrasound-Assisted Extraction of Paeonol from Moutan Cortex: Purification and Component Identification of Extract

“…This might be due to the fact that the polarity of the 60% ethanol was the closest to that of the paeonol, which rendered the paeonol more easily dissolvable [53]. According to the similarity and compatibility [54], when the polarity of the paeonol was similar to that of the ethanol solution, the solubility of paeonol was the highest. When the ethanol concentration was below 60%, the polarity of the ethanol solution increases, leading to decrease in the dissolution of paeonol, while when the ethanol concentration was greater than 60%, the polarity of the ethanol solution decreases, which was not conducive to the dissolution of paeonol.…”