Optimization of the yield of bacteriocin-like substance (BLIS) produced by <i>Pediococcus pentosaceus</i> and its application as food bioconservative

Abstract

Sucrose and inulin were investigated in this study as stimulating agents of bacteriocin production by Pediococcus pentosaceus ATCC 43200 when they were combined with glucose. When such a microbial strain was grown in glucose-based MRS medium, without additional supplements, it reached higher maximum cell concentration (2.68 g/L) and generation time (2.17 h), but lower specific growth rate (0.32 h-1) than in the same medium supplemented with 1.0% of both ingredients (2.53 g/L, 1.60 h and 0.43 h-1 , respectively). Glucose replacement by sucrose or inulin almost completely suppressed growth, hence confirming that it is the preferred carbon source. Qualitatively, similar results were observed for lactate production, which was 59.8% higher in glucose-based medium. Enterococcus and Listeria strains were sensitive to bacteriocin, and both supplements in the glucose-based MRS medium improved the antimicrobial effect against these strains, and were also able to speed up P. pentosaceus in the exponential phase.