2022
DOI: 10.3390/plants11081067
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Optimization of the Transformation Protocol for Increased Efficiency of Genetic Transformation in Hevea brasiliensis

Abstract: The recurring growth of bacterium in newly developed resistant cells and a minimal level of bacterial infection rate are the main limiting factors of Agrobacterium-mediated transformation experiments in Hevea brasiliensis. The current study aimed to optimize crucial factors of the transformation protocol in order to obtain an efficient transformation experimental model for Hevea using cotyledonary somatic embryos as explants. Transformation conditions such as antibiotic concentration, preculture duration, Agro… Show more

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Cited by 6 publications
(6 citation statements)
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“…In addition, the induction of somatic embryo from anther callus is somewhat difficult, which also impedes the genetic transformation efficiency using anther as explants. Alternatively, SEs can be used as explants to continuously provide plentiful receptor materials by highly efficient cyclic proliferation, and with relatively high transformation efficiency (Hua et al ., 2010; Udayabhanu et al ., 2022). Appropriate screening markers are crucial for genetic transformation efficiency, kanamycin is usually used as a suitable selection agent to screen transgenic cell lines in dicotyledonous species, including Hevea (Jayashree et al ., 2003; Kala et al ., 2014; Huang et al ., 2015), however, the impact of different screening markers on somatic embryo transformation has not been systematically studied in Hevea somatic embryo transformation.…”
Section: Discussionmentioning
confidence: 99%
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“…In addition, the induction of somatic embryo from anther callus is somewhat difficult, which also impedes the genetic transformation efficiency using anther as explants. Alternatively, SEs can be used as explants to continuously provide plentiful receptor materials by highly efficient cyclic proliferation, and with relatively high transformation efficiency (Hua et al ., 2010; Udayabhanu et al ., 2022). Appropriate screening markers are crucial for genetic transformation efficiency, kanamycin is usually used as a suitable selection agent to screen transgenic cell lines in dicotyledonous species, including Hevea (Jayashree et al ., 2003; Kala et al ., 2014; Huang et al ., 2015), however, the impact of different screening markers on somatic embryo transformation has not been systematically studied in Hevea somatic embryo transformation.…”
Section: Discussionmentioning
confidence: 99%
“…Unexpectedly, this is harmful to embryonic induction and development, and successive constant 10 mg/L hygromycin in the medium is the best favourable for embryo transformation. By combining optimized transformation methods (Udayabhanu et al ., 2022) and suitable hygromycin screening strategy, the embryo transformation efficiency is improved from previous 4% (Huang et al ., 2015) to 27% in present study.…”
Section: Discussionmentioning
confidence: 99%
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“…From the results obtained, we confirmed that the phenological impacts were very high on the induction of ABs. Explant age is a crucial factor in the success of plant micropropagation [32,33]. The most suitable phenological stage was the mature period (70.83 ± 7.22), which exhibited the best AB swelling rates compared with the bronze period, color-change period and pale-green period.…”
Section: Discussionmentioning
confidence: 99%
“…Genetic transformation is usually carried by Agrobacterium -mediated method, its transformation efficiency is often influenced by different factors, such as Agrobacterium strains, bacterial density, duration of infection, co-culture time, acetosyringone concentration, and the type of antibiotics [ 20 ], and so on. The gene was successfully transformed into Hevea brasiliensis using cotyledonary somatic embryos as explants [ 21 ], similarly, immature leaf segments of Switchgrass ( Panicum virgatum ) [ 22 ] and leaves of dandelion ( Taraxacum officinale ) were successfully transformed to obtain positive seedlings by optimizing various conditions [ 23 ]. Genetic transformation in sunflower is less reported.…”
Section: Introductionmentioning
confidence: 99%