Optimization of the skin multiple analyte profile bioanalytical method for determination of skin biomarkers from D‐Squame<sup>®</sup> tape samples

Hendrix, S. W.; Miller, K. H.; Youket, Thomas E.; Adam, Ralf; O’Connor, Robert J.; Morel, Jorge G.; Tepper, Bruce E.

doi:10.1111/j.1600-0846.2007.00235.x

Cited by 26 publications

(37 citation statements)

References 40 publications

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“…Biomarkers were extracted in PBS buffer, 0.2% SDS, and 0.5% propylene glycol and sonicated for 60 min at 4°C. Keratin 1,10,11 (as a mixture), keratin 6, involucrin, fibronectin, cortisol, and albumin were quantified using Human Skin Panel Lincoplex Kit microsphere beads (Linco Research, Inc., St. Charles, MO) (13). IL1␣, IL1␤, TNF␣, IL6, MCP1, IL8, IL1RA, INF␥, and IP10 were determined using human cytokine bead-based arrays (Linco Research) with a Bio-Plex multiplex suspension system (BioRad Laboratories, Hercules, CA).…”

Section: Methodsmentioning

confidence: 99%

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Biomarkers of Epidermal Innate Immunity in Premature and Full-Term Infants

et al. 2010

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ABSTRACT:Epidermal innate immunity is a complex process involving a balance of pro-and anti-inflammatory cytokines, structural proteins, and specific antigen presenting cells occurring against a background of neuroendocrine modulators such as cortisol. In this study, a multiplex array system was used to simultaneously determine multiple molecular factors critical for development of epidermal innate immune function from the skin surface of premature and term infants, healthy adults, and vernix caseosa. Samples were analyzed for Keratin 1,10,11, Keratin 6, involucrin, albumin, fibronectin and cortisol, and cytokines IL-1, TNF␣, IL-6, IL-8, MCP1, IP10, IFN␥, and IL-1 receptor antagonist. Keratin 1,10,11 was decreased and involucrin was increased in infants versus adults. All infants had elevated IL1␣ and reduced TNF␣ versus adults. IL-6, IL-8, and MCP1 were significantly increased in premature versus term infants and adults. Skin surface cortisol and albumin were significantly elevated in premature infants. The biomarker profile in premature infants was unique with differences in structural proteins, albumin, and cytokines IL-6, IL-1␤, IL-8, and MCP1. The higher infant IL1␣ may be associated with skin barrier maturation. The significant elevations in skin surface cortisol for preterm infants may reflect a neuroendocrine response to the stress of premature birth. (Pediatr Res 67: 382-386, 2010)

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Section: Methodsmentioning

confidence: 99%

“…Central to this approach is the application of a new noninvasive sampling method (13), which simultaneously determines multiple factors critical for the development of epidermal innate immune function. The technique analyzes biomarkers from the outermost SC eliminating the need for invasive tissue biopsies.…”

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confidence: 99%

Biomarkers of Epidermal Innate Immunity in Premature and Full-Term Infants

et al. 2010

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“…After compression of the tape for 3 seconds, a Pulnix Tm-7CN monochrome CCD camera (Pulnix America Inc., Sunnyvale, CA, USA) and RGB monitor (PVM 1342Q; Sony Co., Tokyo, Japan) were used for measurement of the distribution and density of attached keratinocytes. This result was analyzed using an image analyzer (AIC Inc., Princeton, NJ, USA), and desquamation index (DI) and scaling index (SI) were calculated using this mathematical formula [9,10].…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Korean Red Ginseng in the Treatment of Atopic Dermatitis

Lee

Son²

2011

Journal of Ginseng Research

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In order to determine the efficacy of functional foods, objective measurement of the severity of atopic dermatitis (AD) after taking foods is important. The aim of this study was to conduct an objective evaluation of whether Korean red ginseng (KRG) might be helpful for improvement of skin condition and serum IgE in patients with AD. Thirty atopic patients (18 females and 12 males) participated in this study. Patients took KRG for 16 weeks. Bioengineering methods, including the corneometer and evaporimeter, were used at the start of the study and after 8 weeks and 16 weeks. In addition, we assessed serum IgE levels and the severity scoring of the atopic dermatitis (SCORAD) index. Transepidermal water loss and skin hydration showed significant improvement after 16 weeks. A significant decrease in the SCORAD index, as well as in serum IgE level, was observed after 16 weeks. Our results demonstrated that KRG may be helpful as a functional food for patients with AD.

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“…22À26 In contrast, variations in the gene encoding interleukin-1α (IL-1α) such as the presence of an IL1A-889 T allele have been shown to exert protective effects toward the development of manifest dermatitis that have been attributed to reduced IL-1α levels in the stratum corneum. 27,28 Genetic variations related to the skin barrier that have been found to contribute to increased susceptibility to irritant dermatitis include loss-of-function mutations in the gene encoding the epidermal differentiation protein filaggrin (FLG). The FLG mutations are the most important individual risk factor for atopic dermatitis known so far, 29À31 whereas individuals with atopic skin disease have been found to show enhanced responses to irritant damage.…”

Section: Delayed Acute Irritant Dermatitismentioning

confidence: 99%