Optimization of the separation conditions of tetracyclines on a preselected reversed-phase column with embedded urea group

Abstract

The use of a C12 stationary phase with embedded polar group has been investigated for the separation of seven tetracyclines. The influence of pH, organic modifier, buffer, and temperature on the peak shape and analyte separation was discussed. It appears that all the chromatographic conditions had a great effect on both the resolution and peak shape whereas the elution order was not affected. The baseline separation with symmetrical peaks of the seven tetracyclines can be obtained with a mobile phase containing either 5 mM phosphate buffer pH 2.5/ACN (84:16 v/v) or 5 mM perchlorate buffer pH 2.5/ACN (75:25 v/v) at a temperature not exceeding 20 degrees C. This study reveals that the retention mechanism is ion-pairing.

Polar-Embedded and Polar-Endcapped Stationary Phases for LC

“…2003). A urea linked phase was recently applied to the separation of a mixture of tetracyclines-the dominant retention mechanism was by ion-pairing with buffered mobile phase (Kallel et al 2006). …”

Simultaneous determination of pinoxaden, cloquintocet‐mexyl, clodinafop‐propargyl ester and its major metabolite in barley products and soil using QuEChERS modified with multi‐walled carbon nanotubes coupled with LC–MS/MS

“…The mobile phase dramatically influences the degree of chromatographic separations, ionization efficiency, peak shape, and analysis time (Kallel et al, 2015). To achieve efficient and reliable chromatographic separation and high sensitivity, various mobile phase compositions (deionized water/MeOH, water/ACN, 0.1% FA aqueous solution/ACN, and 2 mM ammonium acetate aqueous solution/ACN) with different gradients were assessed.…”

“…The validation of the proposed LC-MS/MS method was assessed in terms of linearity, sensitivity, ME, precision, and reliability according to CAC GL 90-2017, guidelines on performance criteria for methods of analysis of pesticide residues in food and feed. The matrix-matched calibration curve was used for quantification to avoid inaccurate The mobile phase dramatically influences the degree of chromatographic separations, ionization efficiency, peak shape, and analysis time (Kallel et al, 2015). To achieve efficient and reliable chromatographic separation and high sensitivity, various mobile phase compositions (deionized water/MeOH, water/ACN, 0.1% FA aqueous solution/ACN, and 2 mM ammonium acetate aqueous solution/ACN) with different gradients were assessed.…”