Optimization of the reconstruction of dermal papilla like tissues employing umbilical cord mesenchymal stem cells

Yoo, Bo-Young; Shin, Youn-Ho; Yoon, Hee-Hoon; Seo, Young-Kwon; Song, Kye‐Yong; Park, Jung-Keug

doi:10.1007/s12257-009-3050-z

Cited by 6 publications

(7 citation statements)

References 43 publications

(40 reference statements)

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“…The Korean team of Yoo et al [ 87 – 89 ] examined the effect of hWJSC on the acceleration of wound healing processes along with formation of hair follicles and other skin appendages. Enriched aggregates of hWJSC cells can form new hair follicles.…”

Section: Introductionmentioning

confidence: 99%

“…The addition of growth factors to the culture medium is required: hepatocyte growth factor (HGF) stimulates growth of hair follicles in vivo and in vitro ; basic fibroblast growth factor (bFGF) stimulates growth of dermal papilla cells in vivo ; and vesicular endothelial growth factor (VEGF) stimulates growth of hair follicles and hair root in vivo . The hepatocyte growth factor (HGF) must be used at the stage of differentiation of the dermal papilla in culture, which is relatively expensive [ 87 – 89 ].…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic Potential of Stem Cells in Follicle Regeneration

Owczarczyk‐Saczonek

Krajewska–Włodarczyk

Kruszewska

et al. 2018

Stem Cells International

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Alopecia is caused by a variety of factors which affect the hair cycle and decrease stem cell activity and hair follicle regeneration capability. This process causes lower self-acceptance, which may result in depression and anxiety. However, an early onset of androgenic alopecia is associated with an increased incidence of the metabolic syndrome and an increased risk of the cardiac ischaemic disease. The ubiquity of alopecia provides an encouragement to seek new, more effective therapies aimed at hair follicle regeneration and neoregeneration. We know that stem cells can be used to regenerate hair in several therapeutic strategies: reversing the pathological mechanisms which contribute to hair loss, regeneration of complete hair follicles from their parts, and neogenesis of hair follicles from a stem cell culture with isolated cells or tissue engineering. Hair transplant has become a conventional treatment technique in androgenic alopecia (micrografts). Although an autologous transplant is regarded as the gold standard, its usability is limited, because of both a limited amount of material and a reduced viability of cells obtained in this way. The new therapeutic options are adipose-derived stem cells and stem cells from Wharton's jelly. They seem an ideal cell population for use in regenerative medicine because of the absence of immunogenic properties and their ease of obtainment, multipotential character, ease of differentiating into various cell lines, and considerable potential for angiogenesis. In this article, we presented advantages and limitations of using these types of cells in alopecia treatment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Stem Cells in Follicle Regeneration

Owczarczyk‐Saczonek

Krajewska–Włodarczyk

Kruszewska

et al. 2018

Stem Cells International

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“…Out of these, auto hair transplantation has been the most effective. In addition, because of critical limitations due to cost, amount of hair and number of operations (4), studies on hair regeneration have been attempted using cultured hair follicle cells or mesenchymal stem cells (4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

“…However, DP isolation is technically laborious and difficult, and cultured dermal papilla cells (DPCs) lose their hair inductive abilities after a few passages. To overcome these limitations, studies on the reconstruction of dermal papilla-like tissues have been performed with human umbilical cord-derived mesenchymal stem cells (4,5,7).…”

Section: Introductionmentioning

confidence: 99%

Canine Adipose-Derived Stem Cell Aggregates as a Viable Substitute to Actual Canine Dermal Papillae

Bae

Kim

et al. 2015

Macedonian Veterinary Review

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Hair loss is a major dermatological disease in veterinary and human medicine. Active studies on hair regeneration with mesenchymal stem cells have been performed in an effort to solve the limitations of conservative treatments in human medicine. Our understanding of the canine hair follicle (HF), considering a useful model for the study of the human alopecia, is limited. This study was designed to broaden our understanding of canine dermal papilla (DP), and to reconstruct dermal papilla-like tissue (DPLT) using canine adipose-derived mesenchymal stem cells (AD-MSCs), as an alternative to actual DP. We cultured canine DPs, observed their culture patterns and compared their expression level of DP-related genes and proteins with those of DPLTs by performing RT-PCR analysis and Western blotting. Canine dermal papilla cells (DPCs) showed multilayer culture patterns with pseudo-papillae. Reconstruction of DPLTs was performed successfully. Not only were they morphologically similar to actual DPs, but we also observed similarities between DPCs and DPLTs in molecular characteristics. These findings suggested that DPLT was a viable substitute for DP. This study will not only be helpful for understanding the morphological and molecular characteristics of canine DPCs, but may also serve as a basis for understanding human hair follicle biology and potential therapeutic strategies for alopecia.

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“…These cells may be an attractive therapeutic tool for regenerative medicine and tissue engineering (Aggarwal and Pittenger, 2005; Risbud and Shapiro, 2005; Yoo et al, 2010). Therefore, more recent studies have focused on adult stem cells for future clinical applications (Crop et al., 2009; Gaebel et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

hMSCs possess the potential to differentiate into DP cellsin vivoandin vitro

Sun

Guo

et al. 2012

Cell. Biol. Int. Rep.

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DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

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Optimization of the reconstruction of dermal papilla like tissues employing umbilical cord mesenchymal stem cells

Cited by 6 publications

References 43 publications

Therapeutic Potential of Stem Cells in Follicle Regeneration

Therapeutic Potential of Stem Cells in Follicle Regeneration

Canine Adipose-Derived Stem Cell Aggregates as a Viable Substitute to Actual Canine Dermal Papillae

hMSCs possess the potential to differentiate into DP cellsin vivoandin vitro

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