Optimization of the Process for Producing Biomass and Exopolysaccharide from the King Tuber Oyster Mushroom, Pleurotus tuber-regium (Agaricomycetes), for Biotechnological Applications

Bamigboye, Comfort Olukemi; Oloke, Julius Kola; Burton, Mike. H.; Dames, Joanna F.; Lateef, Agbaje

doi:10.1615/intjmedmushrooms.2019030357

Cited by 16 publications

(5 citation statements)

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“…The study conducted by Supramani [11] reported an optimised production of mycelium biomass of 5.19 g/L and polysaccharides (both EPS [2.64 g/L] and IPS [2.64 g/L]). The study conducted by Bamigboye [24] only showed very low biomass (0.7 g/L) production upon optimising; however, EPS (0.29 g/L) was not significantly improved. Meanwhile, another study conducted by Guo [25] in conventional STR using a faster species Phellinus igniarius generated 13.86 g/L of biomass but with a low level of EPS at 1.92 g/L and suspected strong wall growth.…”

Section: Comparison Of Alsb With Commercially Used Bioreactor and Sha...mentioning

confidence: 86%

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

et al. 2023

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Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation.

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Section: Comparison Of Alsb With Commercially Used Bioreactor and Sha...mentioning

confidence: 86%

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

et al. 2023

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“…Contemporary studies on the growth of the basidiomycete P. ostreatus in liquid media are targeted to the development of submerged culture technology to yield mycelial biomass for use as food supplements, fodders, various physiologically active preparations (Vetvicka et al, 2019;Zhu et al, 2019), and biotechnologically valuable products (Pozdnyakova et al, 2018;Karthikeyan et al, 2019;Noman et al, 2019). The submerged culture technique is commonly recognised as a rapid and efficient method for seeding mycelium production for the wide-scale growth of mushrooms (Bamigboye et al, 2019;.…”

Section: Fungal-azospirillum Interactionsmentioning

confidence: 99%

Edible Mushrooms Could Take Advantage of the Growth-Promoting and Biocontrol Potential of Azospirillum

Tsivileva

Shaternikov

Ponomareva

2022

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Investigations of biologically active substances sourced from xylotrophic macromycetes have shown their fundamental and practical significance. These studies have promoted the development of biotechnologies by obtaining the valuable products from the mycelial biomass and cultivation liquid and using these fungal species in “white chemistry”. Research into environmentally safe biological methods of stimulating the growth of medicinal and edible mushrooms can assist the development of scientific foundations of culture technologies. From the viewpoint of their bio-technological potential, selected factors of biological origin that promote the growth of wide-scale cultivated basidiomycetes are of interest. Improving mushroom resistance against negative environmental impacts seems to be feasible when the mushroom grows in combination with growth-promoting bacteria. The soil bacteria from the Azospirillum genus have attracted attention, as microorganisms are capable of actively influencing the growth and development of agricultural crops. Thus, revealing and exploring the growth-promoting properties of different species of Azospirillum with respect to edible and/or medicinal mushrooms, as well as the properties promoting the suppression of contaminants in double culture, are believed to be feasible and to reveal undoubted novelty. Cultivation jointly with the beneficial bacteria is a more effective biotechnological method for obtaining this valuable functional food faster, with a greater and much better and maintained mushroom yield. Future work involves addressing the problems of mushroom growth promoting bacterial interactions with mushrooms and implementing these synthetic microbial associations into agriculture.

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“…The bioreactor provides optimal conditions such as pH, aeration, temperature, agitation, and dissolved oxygen for the growth of microorganisms and product formation which must be properly controlled [95]. Our research group has carried out a lot of studies in this area; ranging from construction of bioreactors [96,97], through development of bioprocess software, Bi-opro_optimizer [98] for acidification process of yoghurt fermentation [99], fermentation of yeasts using novel feeding strategy [100], optimization of biogas and biohydrogen production [101,102], optimization of citric acid production [103,104], enzyme production [105][106][107], and exopolysaccharide production in mushroom [108,109]. Figure 9 shows the generation of bioreactors produced by our team, while some software interfaces are shown in Figure 10.…”

Section: Fabrication Of Bioreactors Control and Optimization Of Fermentation Processesmentioning

confidence: 99%

Untitled

2021

Nano Plus: Sci. Tech. Nanomat

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This article chronicles the author's research activities in about twenty-two years, and a worthy enterprise of the intertwining patterns of microbiology, biotechnology and nanotechnology to document advances in this area. It details the pioneering roles at initiating researches in biotechnology to produce fructooligosaccharides, and microbial valorization of agrowastes. It also discusses nanobiotechnology where microbes, plants and animals have been used to produce nanoparticles for biomedical, environmental, agricultural and engineering applications. It was affirmed that although microorganisms consist of the good and the bad ones; the pathogenic microbes causing diseases in plants and animals are less than 1% of the hypothetical one trillion types of microorganisms that exist on earth. The contributions of microorganisms and nanoparticles in terms of their product formation were estimated at hundreds of billions of dollars. For instance, the contribution of biotechnology to the world's economy was put at $714.6 billion in 2021. Similarly, estimated cost of nano-based products was put at $2.6 trillion in 2015, with the expectation to reach 3 trillion in 2020. As an industrial microbiologist, the author has employed microbes to produce novel products that included biofertilizer, fructooligosaccharides, citric acid, biogas, industrial enzymes such as laccase, xylanase, keratinase, and fructosyltransferase. Other investigations included biofabrication of metal nanoparticles such as silver, gold, titanium oxide, calcium and silver-gold alloy for diverse applications, as well as fabrication and deployment of bioreactors for growing microorganisms. In a unique manner, a loop connecting basic microbiology, microbial technology and nanotechnology to herald the sub-discipline of microbial nanobiotechnology which is a gold mine was established. This represents a paradigm shift in the fusion of knowledge of immense benefits in developing self-reliant manpower in life sciences. The article underscored the importance of microbiology, noting that the field should be professionalized in Nigeria for the country to reap immense benefits inherent in the attributes and applications of microbes. It was concluded that microbes and nanoparticles although very small, will continue to define the development of man and contribute in no small measure to the world economy and development of creative products to improve the quality of life on earth. Table 3: Applications of biotechnology using the colour coding system Colour Applications Red Health, medical, gene therapy, regenerative medicine, vaccines and antibiotics, developing new drugs, molecular diagnostics techniques Yellow Food biotechnology, nutrition science Blue Aquaculture, coastal and marine biotechnology Green Agricultural, biofertilizers, biopesticides, transgenic plants and animals Brown Arid zone and desert biotechnology Dark Bioterrorism, biowarfare, biocrimes, anticrop warfare Purple Patents, publications, inventions, intellectual property rights White Gene-based bioindu...

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Optimization of the Process for Producing Biomass and Exopolysaccharide from the King Tuber Oyster Mushroom, Pleurotus tuber-regium (Agaricomycetes), for Biotechnological Applications

Cited by 16 publications

References 0 publications

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

Edible Mushrooms Could Take Advantage of the Growth-Promoting and Biocontrol Potential of Azospirillum

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