Optimization of the Extraction Conditions and Simultaneous Quantification by RP-LC of Six Alkaloids in Evodiae Fructus

Zhao, Mei‐Xin; Yang, Xiu‐Wei

doi:10.1365/s10337-008-0543-4

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…Each experiment trial was conducted three times. The peak area of analytes can be used as a response function because it can take into consideration the effect of changes in the variables on the extraction efficiency of this technique [28]. In the present study, the effect of variables on the summed total area of all seven alkaloids, the most abundant compound (berberine), and the least abundant compound (evodiamine) was investigated, and used as the performance indicator.…”

Section: Optimization Of the Ase Extraction Conditionsmentioning

confidence: 99%

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Simultaneous analysis of seven alkaloids in Coptis‐Evodia herb couple and Zuojin pill by UPLC with accelerated solvent extraction

Gào

Yang

Marriott

2010

J of Separation Science

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A rapid, sensitive, and accurate ultra-performance liquid chromatography-UV detection method was developed and validated for simultaneous determination of seven active alkaloids, berberine, palmatine, coptisine, jatrorrhizine, epiberberine, evodiamine, and rutaecarpine, in the Coptis-Evodia herb couple and the Zuojin pill preparation. The original validated method had a total time of about 11 min, which was reduced to 4.7 min by further optimization of gradient elution conditions with eluent A comprising water/methanol/formic acid (v/v/v, 90:10:0.1) and eluent B ACN. An accelerated solvent extraction method was used for the rapid extraction of herb products and pill preparations. Linear behavior over the investigated concentration ranges was observed, with values of r(2) greater than 0.9987 for all analytes. Experiment results were satisfactory for both intra-day and inter-day precision, and method accuracy. Method recovery ranged from 95.7 to 103.5% based on pure reference standards. The validated method was successfully applied to determine amounts of bioactive alkaloid compounds in five samples, in the Coptis-Evodia herb couple, and the commercial Zuojin pill products. The developed method achieved baseline resolution for all seven main basic compounds and is suitable for use as a routine procedure for the rapid identification and quantification of basic compounds in the Coptis-Evodia herb couple and related products.

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Section: Optimization Of the Ase Extraction Conditionsmentioning

confidence: 99%

“…Some methods have been reported for the determination of some of the indolequinoline alkaloids in Evodia crude drugs such as TLC [24], CE [25], HPLC [26][27][28], and HPLC-MS [29].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of seven alkaloids in Coptis‐Evodia herb couple and Zuojin pill by UPLC with accelerated solvent extraction

Gào

Yang

Marriott

2010

J of Separation Science

Self Cite

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“…In addition, coptisine and palmatine were previously tested for skin permeation enhancement [13] and antimicrobial activities with promising results [12]. The characteristic chemical constituents in E. fructus, the indolequinoline alkaloids evodiamine and rutaecarpine, are considered to have many beneficial effects on human health [14]. Pharmacological and clinical studies indicated that evodiamine and rutaecarpine are bioactive compounds with reported vasodilatory [15], anti-inflammatory [16], and analgesia [17] activities.…”

Section: Introductionmentioning

confidence: 99%

“…However, up to now, very limited studies on Zuojin pill extract have been carried out in the pharmaceutical sciences. The protoberberine alkaloids in C. rhizoma and indolequinoline alkaloids in E. fructus were analyzed by high-performance liquid chromatography (HPLC) separately [14,23,24]. Several methods were reported for the determination of protoberberine and indolequinoline alkaloids in herb couple of C. rhizoma and E. fructus, such as HPLC [25], HPLC-mass spectrometry (MS) [3], and capillary electrophoresis (CE) [26].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of protoberberine and indolequinoline alkaloids in Zuojin pill extract by high-performance liquid chromatography

Zhang

Wang

2013

Acta Chromatographica

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“…Benth. [1][2][3] Evo and Rut have been reported to exhibit anti-inflammatory 4 and anti-nociceptive 5 effects. Both Evo and Rut have been found to strongly inhibit prostaglandin E2 synthesis from lipopolysaccharide-treated RAW 264.7 cells, and Evo has also been shown to inhibit cyclooxygenase-2 induction and NF-κB activation.…”

Section: Introductionmentioning

confidence: 99%

In vitro cellular uptake of evodiamine and rutaecarpine using a microemulsion

Zhang

Huang²,

Zhang³

et al. 2012

IJN

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Objective: To investigate the cellular uptake of evodiamine and rutaecarpine in a microemulsion in comparison with aqueous suspensions and tinctures. Materials and methods: A microemulsion was prepared using the dropwise addition method. Mouse skin fibroblasts were cultured in vitro to investigate the optimal conditions for evodiamine and rutaecarpine uptake with different drug concentrations and administration times. Under optimal conditions, the cellular uptake of microemulsified drugs was assayed and compared to tinctures and aqueous suspensions. Rhodamine B labeling and laser scanning confocal microscopy (LSCM) were used to explore the distribution of fluorochrome transferred with the microemulsion in fibroblasts. Cellular morphology was also investigated, using optical microscopy to evaluate microemulsion-induced cellular toxicity. Results:The maximum cellular drug uptake amounts were obtained with a 20% concentration (v/v) of microemulsion and an 8 hour administration time. Drug uptake by mouse skin fibroblasts was lowest when the drugs were loaded in microemulsion. After incubation with rhodamine B-labeled microemulsion for 8 hours, the highest fluorescence intensity was achieved, and the fluorochrome was primarily distributed in the cytochylema. No obvious cellular morphologic changes were observed with the administration of either the microemulsion or the aqueous suspension; for the tincture group, however, massive cellular necrocytosis was observed. Conclusion:The lower cellular uptake with microemulsion may be due to the fact that most of the drug loaded in the microemulsion vehicle was transported via the intercellular space, while a small quantity of free drug (released from the vehicle) was ingested through transmembrane transport. Mouse skin fibroblasts rarely endocytosed evodiamine and rutaecarpine with a microemulsion as the vehicle. The microemulsion had no obvious effect on cellular morphology, suggesting there is little or no cellular toxicity associated with the administration of microemulsion on mouse skin fibroblasts.

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Optimization of the Extraction Conditions and Simultaneous Quantification by RP-LC of Six Alkaloids in Evodiae Fructus

Cited by 21 publications

References 13 publications

Simultaneous analysis of seven alkaloids in Coptis‐Evodia herb couple and Zuojin pill by UPLC with accelerated solvent extraction

Simultaneous analysis of seven alkaloids in Coptis‐Evodia herb couple and Zuojin pill by UPLC with accelerated solvent extraction

Simultaneous analysis of protoberberine and indolequinoline alkaloids in Zuojin pill extract by high-performance liquid chromatography

In vitro cellular uptake of evodiamine and rutaecarpine using a microemulsion

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