Optimization of the discrete pulse sequence for two-dimensional FT-ICR mass spectrometry using infrared multiphoton dissociation

Agthoven, Maria A. van; Chiron, Lionel; Coutouly, Marie Aude; Sehgal, Akansha Ashvani; Pelupessy, Philippe; Delsuc, Marc-André; Rolando, Christian

doi:10.1016/j.ijms.2014.06.019

Cited by 29 publications

(72 citation statements)

References 29 publications

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“…Despite the optimization of the pulse sequence [17], vertical lines are visible along the most intense peaks corresponding to scintillation noise and curves corresponding to harmonics. However, the signal-to-noise ratio of the data was sufficient and the precursors were simple enough that denoising was not deemed necessary to interpret the 2D mass spectrum [16].…”

Section: Resultsmentioning

confidence: 99%

“…Finally, the artifact peak at m/z 217.39 is present in both product-ion spectra shown in Figure 1b and c and is absent in Figure 2b. Owing to the radial modulation of the precursor ions in the pulse sequence for 2D FT-ICR MS, artifacts do not show peaks in the horizontal fragment scan as actual fragments would do [17]. As a result, m/z 217.39 can be ruled out as a fragment ion, and as its frequency is approximately 850 kHz, can be assigned to rf interference from the multipoles.…”

Section: Resultsmentioning

confidence: 99%

“…In 2010, 2D FT-ICR MS using the original pulse sequence was implemented again using IRMPD [13] and electron capture dissociation (ECD) [14] for peptides from a nanoelectrospray ion source. Advances in data processing and denoising algorithms were made [15,16], and the pulse sequence was optimized in order to maximize signal-to-noise ratios [17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differentiating Fragmentation Pathways of Cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Agthoven

Barrow

Chiron³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry is a data-independent analytical method that records the fragmentation patterns of all the compounds in a sample. This study shows the implementation of atmospheric pressure photoionization with two-dimensional (2D) Fourier transform ion cyclotron resonance mass spectrometry. In the resulting 2D mass spectrum, the fragmentation patterns of the radical and protonated species from cholesterol are differentiated. This study shows the use of fragment ion lines, precursor ion lines, and neutral loss lines in the 2D mass spectrum to determine fragmentation mechanisms of known compounds and to gain information on unknown ion species in the spectrum. In concert with high resolution mass spectrometry, 2D Fourier transform ion cyclotron resonance mass spectrometry can be a useful tool for the structural analysis of small molecules.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differentiating Fragmentation Pathways of Cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Agthoven

Barrow

Chiron³

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…IR photons were produced at a wavelength of 10.6 μm and pulsed into the ICR cell for 0.3 s prior to detection. The pulse program used for the 2D MS analysis was optimized in order to have the highest fragmentation efficiency according to previous studies [30].…”

Section: Ft-icr Ms Was Performed On a 12 T Bruker Solarix Ft-icrmentioning

confidence: 99%

2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach

Floris

Agthoven

Chiron³

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time-and sample-consuming experiment than doing the same thing using sequential MS/MS experiments.

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“…Since 2010, thanks to improvements in computational capacities for data processing and storage, 2D MS on FT‐ICR instruments has been developed into a fully‐fledged analytical technique with infrared multiphoton dissociation (IRMPD) and electron capture dissociation (ECD) as fragmentation methods . De‐noising algorithms have been developed in order to reduce the effects of scintillation noise in 2D mass spectra .…”

Section: Methodsmentioning

confidence: 99%