Optimization of the arsenazo-III method for the determination of uranium in water and plant samples

Jauberty, Loïc; Drogat, Nicolas; Decossas, Jean-Louis; Delpech, V.; Gloaguen, Vincent; Sol, Vincent

doi:10.1016/j.talanta.2013.06.046

Cited by 33 publications

(9 citation statements)

References 21 publications

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“…The Environmental Protection Agency (EPA) limits the level of uranium in drinking water as 30 lg L -1 [1]. This maximum contaminant level is consistent with WHO recommendation value [3]. This stringent level regulation made scientist develop new analytical techniques for accurate determination uranium in trace levels in water samples.…”

Section: Introductionsupporting

confidence: 56%

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Ionic liquid dispersive microextraction and spectrophotometric determination of trace uranyl ion in water samples

Bağda

Tüzen

2015

J Radioanal Nucl Chem

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In the present study, spectrophotometric determination of uranyl with 2-(5-Bromo-2-pyridylazo)-5-(diethylamino) phenol after ionic liquid based microextraction has been developed. Effect of experimental parameters were optimized to achieve high sensitivity and stability of the complex. The analytical parameters such as linear range (1.33-670 lg L -1 ), limit of detection (0.97 lg L -1 ) and enrichment factor (150) were calculated for the method. The relative standard deviation of ten replicate measurement of uranyl was 6.53 and 2.59 % for 66 and 133 lg L -1 uranium, respectively. The proposed method was used to determine the uranyl ions in spiked water samples.

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Section: Introductionsupporting

confidence: 56%

“…It can be naturally found in environmental soil, rock, air and water samples [2]. According to Jouberty et al [3], it is slightly radioactive element in its natural state, but it is nonetheless harmful to body. The Environmental Protection Agency (EPA) limits the level of uranium in drinking water as 30 lg L -1 [1].…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid dispersive microextraction and spectrophotometric determination of trace uranyl ion in water samples

Bağda

Tüzen

2015

J Radioanal Nucl Chem

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“…The potentials were measured by using the electrode cell assembly under optimized conditions described in this work and calibration plots were further employed to evaluate the concentration of UO 2 2+ in these samples. The results were validated using the results of the analyses of the same samples by applying Arsenazo III spectrophotometric method and the results obtained are presented in Table . As can be seen, the results obtained from both techniques are comparable, indicating the applicability of the developed electrode for accurate and precise determination of UO 2 2+ content in real samples.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Selective Determination of Uranyl Ions Using PVC Membrane Sensor

Akl

2017

Electroanalysis

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A UO22+‐PVC membrane electrode was constructed using 2‐thenoyltrifluoroacetone as ionophore and its electrochemical response performance was characterized. The effect of membrane composition on the electrode performance was studied and best results were obtained using dioctylsebacate as a mediator and potassium tetrakis(4‐chlorophenyl)borate as anion excluder. The optimized UO22+‐sensor exhibited a Nernstian response with a slope of 29.5±0.5 mV decade−1 over the concentration range 5.0×10−7−1.0×10−1 mol L−1 at 25 °C with a detection limit of 3.1×10−7 mol L−1. The optimized electrode showed very good selectivity towards UO22+ relative to a wide variety of other cations and fast response time. Surface morphology of the optimized membrane electrode at different stages of its development and use was investigated and discussed. Quantum chemical calculations for geometrical optimization of the ionophore were carried out to investigate the interaction between the ionophore and UO22+ using DFT B3LYP/6‐31++G(d,p) level of theory and the obtained data confirmed the proposed response mechanism. The developed sensor was successfully applied for UO22+ selective determination in real water samples and the obtained results were compared to those obtained by spectrophotometric method indicating no significant difference.

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“…Content of U (VI) was determined by the arsenazo (III) staining method [29]. 1 mL of sample was added to 1 mL of arsenazo (III) chromogenic agent, the volume of the mix was adjusted to 10 mL with 0.4 mol/L chloroacetic acid-sodium chloroacetate buffer, then the mix was shaken and left to incubate at room temperature for 30 min.…”

Section: Methodsmentioning

confidence: 99%

ytiB and ythA Genes Reduce the Uranium Removal Capacity of Bacillus atrophaeus

Wang

Xiao

Chen

et al. 2019

IJMS

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Two Bacillus atrophaeus strains, the first being a highly stress-resistant ATCC 9372 strain and the Ua strain identified from a chromium mine by our lab, differ in their abilities to tolerate and remove Uranium (VI) from contaminated water. An increase in U(VI) concentration in growth media led to a decrease in the tolerance and bio-remedial capacity of both strains. However, under high concentrations of U(VI) in the growth media, the ATCC 9372 strain demonstrated a higher tolerance and a higher removal capacity than the Ua strain. Two approaches, transcriptome sequencing and transgenic technology, were used to elucidate the relationship between particular genes within these two strains and their U(VI) removal capacity. Sequencing confirmed the expression of two genes unique to the Ua strain, previously designated ytiB and ythA. They encode putative proteins that show the highest levels of identity to carbonic anhydrase and cytochrome bd terminal oxidase I, respectively. Using the pBE-S DNA vector, ytiB and ythA were transformed into the ATCC 9372 strain of Bacillus atrophaeus. Under a U(VI) concentration of 120 mg/L, the removal rates of the transgenic ATCC 9372-ytiB and ATCC 9372-ythA strains decreased by 7.55% and 7.43%, respectively, compared to the removal rate of the control strain transformed with empty plasmid. The results suggest that both ythA and ytiB genes have a negative influence on the uranium removing capacity of Bacillus atrophaeus. This finding will help to elucidate the molecular mechanisms of uranium removal by bacteria.

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Optimization of the arsenazo-III method for the determination of uranium in water and plant samples

Cited by 33 publications

References 21 publications

Ionic liquid dispersive microextraction and spectrophotometric determination of trace uranyl ion in water samples

Ionic liquid dispersive microextraction and spectrophotometric determination of trace uranyl ion in water samples

Electrochemical Selective Determination of Uranyl Ions Using PVC Membrane Sensor

ytiB and ythA Genes Reduce the Uranium Removal Capacity of Bacillus atrophaeus

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