Optimization of the Amplicons Detection System of Loop-Mediated Isothermal Amplification on Microfluidic Compact Disk

Uddin, Shah Mukim; Ibrahim, Fatimah; Cho, Jongman; Thong, Kwai Lin

doi:10.1007/978-981-10-4505-9_2

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…In the past decade, research exploring the potentials to translate the LAMP-on-tube technique into LAMP-on-chip due to its unique advantage of single amplification temperature and low reagent cost [ 58 ]. Several lab-on-a-disk-based LAMP devices have been developed integrating preloaded reagent mixes and detection agents [ 52 , 78 , 79 , 80 ]. Researchers have explored paper-based devices for their robustness, cost-effectiveness, and user-friendliness [ 81 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity

Uddin

Sayad

Chan

et al. 2021

Sensors

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HLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loop-mediated isothermal amplification (LAMP) on the tube, which requires two-stage device development to translate into a portable platform. Here, we report a heater-integrated lab-on-a-chip device for the LAMP amplification, which can rapidly detect HLA-B alleles colorimetrically. A gold-patterned micro-sized heater was integrated into a 3D-printed chip, allowing microfluidic pumping, valving, and incubation. The performance of the chip was tested with color dye. Then LAMP assay was conducted with human genomic DNA samples of known HLA-B genotypes in the LAMP-chip parallel with the tube assay. The LAMP-on-chip results showed a complete match with the LAMP-on-tube assay, demonstrating the detection system’s concurrence.

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