Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide

Yu-bin, JI; Dong, Fang; Lang, Lang; Zhang, Lingwen; Miao, Jing; Liu, Zhenfeng; Jin, Lina; Ying, Hao

doi:10.3390/ijms131217275

Cited by 23 publications

(21 citation statements)

References 17 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It had also been documented that the anti-tumor activities of selenized polysaccharides depended on its Se content. Ji et al reported that seleno-Capparis spionosa polysaccharide (Se-CSPs, Se content of 5518 μg/g, molecular mass data was not given) obtained from selenylation could significantly increased cytotoxicity to human gastric cancer SGC-7901 cells with the IC50 value of 116 μg/mL (Ji et al, 2012). A selenized pectic polysaccharide from Codonopsis pilosula (sCPP1b, molecular mass data was not given) with low selenium content of 478.17 μg/g was obtained in HNO3-Na2SeO3 method.…”

Section: Anti-tumor Activity In Vitromentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

View full text Add to dashboard Cite

It has been reported in our previous work that selenized Artemisia sphaerocephala polysaccharides (SeASPs) with the Se content range of 168-1703μg/g were synthesized by using Na2SeO3/HNO3/BaCl2 system. In the present work, the solution property of SeASP was studied by using size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS). A decrease in df values indicated that SeASPs with different conformational features that were highly dependent on MW. SeASPs exhibited a more rigid conformation (df value of 1.29-1.52) in low molecular weight range (MW of 1.026-1.426×10(4)g/mol) and compact spherical conformation in high molecular weight range (MW of 2.268-4.363×10(4)g/mol). It could be due to the degradation of polysaccharide chains in HNO3, which was supported in monosaccharide composition analysis. Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results also confirmed the conformational transition and the evidence on the shape of the rigid chains. In vitro anti-tumor assays, SeASP2 displayed greater anti-proliferative effects against three tumor cell lines (hepatocellular carcinoma HepG-2 cells, lung adenocarcinom A549 cells and cervical squamous carcinoma Hela cells) in a dose-dependent manner. This suggested that selenylation could significantly enhance the anti-tumor activities of polysaccharide derivatives in vitro.

show abstract

Section: Anti-tumor Activity In Vitromentioning

confidence: 99%

“…Capparis spionosa polysaccharide of 5.547 mg/g (Ji et al, 2012), garlic polysaccharide of 29.4 mg/g (Gao et al, 2016) and pectic polysaccharide of 478.17 μg/g (Chen et al, 2014b). The selenylation of polysaccharide is one of the most intriguing scientific endeavors in carbohydrate chemistry.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

View full text Add to dashboard Cite

show abstract

“…The substitution position, molecular weight and solution conformation were characterized by employing 13 C NMR spectroscopy, size-exclusion chromatograph combined with multi-angle laser light scattering photometer (SEC-MALLS), congo red (CR) spectrophotometric analysis and atomic force microscopy (AFM) observation. After selenylation, SeASP exhibited better scavenge hydroxyl and superoxide radical activities in vitro [24].…”

Section: Introductionmentioning

confidence: 99%

“…Fourier transform infrared spectroscopy (FT-IR), 13 C NMR spectroscopy, SEC-MALLS were used to study the chemical structure and conformation parameter of SeASP MW . AFM was employed to observe the shape and size of the polysaccharide molecules.…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Wang

Yang

Bao

et al. 2017

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

In this work, selenylation of Artemisia sphaerocephala polysaccharide (SeASP) was studied by using HSeO/HNO/BaCl reaction system in microwave field. SeASP exhibited the Se content range of 111-264μg/g with high yields (72.1-94.9%). C NMR results indicated that the weak C-6 substitution was occurred. The decrease (from 7.348×10g/mol to 1.736-4.667×10g/mol) in weight average molecular mass (M) of SeASP was observed in size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS) analysis. SeASP exhibited a more rigid solution conformation which might be due to the degradation of polysaccharide chains in acidic reaction reagent. This was also supported by atomic force microscopy (AFM) result that SeASP showed short chains and island-like topography. In anti-tumor activity assays, SeASP exhibited the inhibition rates of 32.381% and 39.776% against human non-small cell lung cancer cell line (H1650) at the concentration of 100 and 200μg/mL, respectively. The relatively weak inhibition effect of SeASP was not related to cell apoptosis and cell cycle arrest, suggesting Se content might be a key factor to influence the anti-tumor activities of selenized polysaccharides in vitro.

show abstract

“…The study have confirmed that many of its chemical constituents have anti-oxidative, antimicrobial and many others properties. Ji et al reported that, Capparis spionosa L. contains various active components such as glucose isothiocyanates, volatile oil, sugar ligands and alkaloid compounds that is used for controlling and treating diseases such as hypertension and rheumatoid arthritis [6].…”

Section: Introductionmentioning

confidence: 99%

Early Feasibility Study on Capparis Production and Processing in Hamedan Province in Iran

Khoram¹,

Reyahi-Khoram²

2018

JOAAT

View full text Add to dashboard Cite

Capparis spinosa is one of the most widely consumed aromatic and medicinal plants in Iran whose local name is "shapaleh". The aim of the present study was to evaluate whether the production and processing of Capparis in Asadabad Township in Hamedan province is suitable and sustainable. The food processing industry is one of the basic industries operating in Iran and is divided into several sections. Iran is the second largest economy in the Middle East and North Africa, Iran annually produces 12 million tones of fresh fruits and 23 million tons vegetables. In this situation, it is necessary to select kind of crop, technology tools and apply methods to improve them. Today in Iran, there are three industrial companies which are engaged in industrial processing of Capparis. One of the mentioned industries is situated in Hamedan province with name "Sahar Food Industries Company". It is concluded that, if local authorities are honest about their needs, Stakeholders will be able to give feedback, receive information and be informed of major decisions made.

show abstract

Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide

Cited by 23 publications

References 17 publications

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Early Feasibility Study on Capparis Production and Processing in Hamedan Province in Iran

Contact Info

Product

Resources

About