Optimization of Some Variables of on-Line Injection in Pressurized Planar Electrochromatography

2022

Molecules

In our previous paper we have presented the new prototype equipment and introduced a new analytical technique—high-performance/high-pressure layer electrochromatography (HPLEC), a combination of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC). In this paper, the work of the equipment in various operational modes is investigated. Some difficulties and challenges related to various aspects of separation are discussed. The OPLC and HPLEC techniques are compared in terms of selectivity and performance. The results show that our equipment can be successfully used for singe- and multichannel OPLC and HPLEC separations in various sample application and detection modes. It includes the high-throughput, multichannel, and fully automated online separation of multiple samples simultaneously. The equipment allows for the independent optimization of various operational parameters. HPLEC combines the advantages of column/capillary and planar separation techniques while overcoming their limitations. It also combines the advantages and overcomes the drawbacks of OPLC and PPEC. It provides hydrodynamic flow of the mobile phase, irrespective of the voltage used and/or the mobile phase composition. Thus, any optimization of the composition and the voltage can be used independently. Both can be used to obtain the required selectivity of separation. The voltage can be used to facilitate the mobile phase flow and accelerate the analysis.

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Section: Introductionmentioning

confidence: 99%

Comparison of Overpressured-Layer Chromatography and High-Performance/High-Pressure Layer Electrochromatography Using the New Prototype Equipment in Various Operational Modes

2022

Molecules

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“…As in the case of OPLC, commercially available chromatographic plates can be adapted to PPEC by sealing their edges. Most papers on PPEC present offline sample application (with the exception of refs and ) and, so far, only offline sample detection. This technique has been successfully used for separation of many various types of compounds, and its advantages make it an interesting alternative or supplement to the other separation techniques. − , However, as indicated in one of our latest papers, it has one serious drawback.…”

mentioning

confidence: 99%

High Performance (High Pressure) Layer Electrochromatography Separation Technique: Equipment and Preliminary Results

2022

Anal. Chem.

We present a new prototype device and propose a new analytical technique: high performance (high pressure) layer electrochromatography (HPLEC). The equipment provides a combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC), yet it still enables researchers to perform each of these analyses separately. In comparison to PPEC, HPLEC provides hydrodynamic flow of the mobile phase, irrespective of the voltage used and the mobile phase composition. The advantages of HPLEC over OPLC include the possibility of the use of the electrophoretic effect to influence the selectivity of separation and the use of the electroosmotic effect to facilitate the mobile phase flow in order to decrease backpressure and increase the flow velocity. Many operational parameters can be freely adjusted and optimized independently. The equipment is fully automated and can work in various separation/operational modes, including combinations of online/offline sample application and detection. We present preliminary results of simultaneous, fully online, multichannel HPLEC separation of analgesic drugs (including acetaminophen, ibuprofen, and tramadol) as an example of increasing analysis throughput.

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Pressurized Planar Electrochromatography

Hałka-Grysińska

Instrumental Thin-Layer Chromatography

2015