Optimization of solvent extraction of antioxidants from Eucalyptus globulus leaves by response surface methodology: Characterization and assessment of their bioactive properties

Gullón, Beatriz; Gullón, Patricia; Lú-Chau, Thelmo A.; Moreira, Marı́a Teresa; Lema, Juan M.; Eibes, Gemma

doi:10.1016/j.indcrop.2017.07.014

Cited by 75 publications

(47 citation statements)

References 38 publications

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“…(Figure 3a), and again this was the most important factor. Similar influence of the ethanol concentration in flavonoid extraction has been observed in other biomasses [2,18]. In this work, the model predicted the greatest TFC at 42.3% ethanol concentration, 70% amplitude, and 15 min., which would achieve 126.9 mg RE/g EOP.…”

Section: Total Flavonoid Compounds (Tfc)supporting

confidence: 84%

“…As presented in Figure 2a, the ethanol concentration had a positive influence that reached a maximum at approximately 40% ethanol and subsequently declined, as indicated by the negative signs of the linear and quadratic terms for this parameter. This behaviour can be explained in accordance with the principle of similarity and intermiscibility; i.e., greater extraction of phenolic compounds is achieved when the solvent and the solute have similar polarity [2]. Notably, the use of a mixture of ethanol and water, as the solvent is more efficient in the phenolic extraction than the use of a pure solvent.…”

Section: Influence Of the Factors On The Uae Of Eop And Model Equationssupporting

confidence: 55%

“…In recent years, the demand for natural antioxidants as alternatives to synthetic antioxidants has increased. Phenolic compounds, such as flavonoids, phenolic acids, phenolic alcohols, stilbenes, lignans, and tannins, exhibit antioxidant activities and other types of bioactivity (anti-inflammatory, anticarcinogenic, antiviral, hepatoprotective, and neuroprotective activities, among others) [1,2]. These properties make these compounds useful in the food and pharmaceutical industries as food preservatives and biocompounds for human health [3].…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Extraction as a First Step in a Biorefinery Strategy for Valorisation of Extracted Olive Pomace

et al. 2019

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Currently, interest in finding new feedstock as sources of natural food antioxidants is growing. The extracted olive pomace (EOP), which is an agro-industrial residue from the olive pomace extracting industries, is generated yearly in big amounts, mainly in the Mediterranean countries. EOP was subjected to an ultrasound assisted extraction with ethanol-water mixtures. The effect of main parameters, such as ethanol concentration (30–70% v/v), ultrasound amplitude (20–80%), and extraction time (5–15 min), on the extraction of antioxidant compounds was evaluated according to a Box–Behnken experimental design. The antioxidant capacity of the resulting extracts was determined by measuring their content in total phenolic compounds (TPC) and flavonoids (TFC), as well as their antioxidant activity by DPPH, ferric reducing antioxidant power (FRAP), and ABTS assays. Considering the simultaneous maximization of these five responses, the optimal conditions were found to be 43.2% ethanol concentration, 70% amplitude, and 15 min. The ultrasound assisted extraction of EOP under these optimized conditions yielded an extract with a phenolic and flavonoid content (per gram of EOP) of 57.5 mg gallic acid equivalent (GAE) and 126.9 mg rutin equivalent (RE), respectively. Likewise, the values for DPPH, ABTS, and FRAP assay (per gram of EOP) of 56.7, 139.1, and 64.9 mg Trolox equivalent, respectively were determined in the optimized extract.

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Section: Total Flavonoid Compounds (Tfc)supporting

confidence: 84%

Section: Influence Of the Factors On The Uae Of Eop And Model Equationssupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Extraction as a First Step in a Biorefinery Strategy for Valorisation of Extracted Olive Pomace

et al. 2019

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“…Since it was not possible to measure the concentration of rutin oligomers in solution (no standards are available), an indirect protocol for measuring apparent aqueous solubility was developed based on the total flavonoid content (TFC) assay explained by Kim et al [35] with the modifications proposed by Gullón et al [36].…”

Section: Apparent Solubility Of Rutin Oligomersmentioning

confidence: 99%

Laccase Activity as an Essential Factor in the Oligomerization of Rutin

et al. 2018

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The enzyme-mediated polymerization of bioactive phenolic compounds, such as the flavonoid rutin, has gained interest due to the enhanced physico-chemical and biological properties of the products, which increases their potential application as a nutraceutical. In this work, the influence of enzyme activity on rutin oligomerization was evaluated in reactions with low (1000 U/L) and high (10,000 U/L) initial laccase activities. For both reactions, high molecular weight oligomer fractions showed better properties compared to lower weight oligomers. Products of the reaction with low laccase activity exhibited thermal stability and antioxidant potential similar to control reaction, but led to higher inhibitory activity of xanthine oxidase and apparent aqueous solubility. Oligomers obtained in the reaction with high laccase activity showed better apparent aqueous solubility but decreased biological activities and stability. Their low antioxidant activity was correlated with a decreased phenolic content, which could be attributed to the formation of several bonds between rutin molecules.

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“…Using pyrolysis and gas chromatography coupled to mass spectrometry (Py-GC/MS) analysis, Gullón et al [ 41 ] identified 21 compounds (β-eudesmol, γ-eudesmol, and globulol, among others) from Eucalyptus leaf extract. The authors also conducted a Fourier-transform infrared spectroscopy (FTIR) analysis, revealing specific bands that can be attributed to different phenolic compounds such as flavonoids and polyphenols.…”

Section: Phenolic Compounds From Eucalyptus Spementioning

confidence: 99%

Value-Added Compound Recovery from Invasive Forest for Biofunctional Applications: Eucalyptus Species as a Case Study

Gullón

Astray

et al. 2020

Molecules

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From ancient times, the medicinal properties of the different Eucalyptus species are well known. In fact, plants from this family have been used in folk medicine as antiseptics, and to treat different ailments of the upper respiratory tract such as sinus congestion, common cold, or influenza. Moreover, other biological activities were described for Eucalyptus species such as antioxidant and antimicrobial properties. In the last few decades, numerous investigations revealed that the compounds responsible for these properties are secondary metabolites that belonging to the group of phenolic compounds and are present in different parts of the plants such as leaves, bark, wood, fruits, and stumps. The increasing demand for natural compounds that can substitute synthetic antioxidants and the increase in resistance to traditional antibiotics have boosted the intense search for renewable natural sources containing substances with such bioactivities, as well as greener extraction technologies and avant-garde analytical methods for the identification of the target molecules. The literature data used in this paper were collected via Scopus (2001–2020) using the following search terms: Eucalyptus, extraction methods, phenolic compounds, and biological activities. This review collects the main studies related to the recovery of value-added compounds from different Eucalyptus species, as well as their biofunctional applications.

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Optimization of solvent extraction of antioxidants from Eucalyptus globulus leaves by response surface methodology: Characterization and assessment of their bioactive properties

Cited by 75 publications

References 38 publications

Ultrasound-Assisted Extraction as a First Step in a Biorefinery Strategy for Valorisation of Extracted Olive Pomace

Ultrasound-Assisted Extraction as a First Step in a Biorefinery Strategy for Valorisation of Extracted Olive Pomace

Laccase Activity as an Essential Factor in the Oligomerization of Rutin

Value-Added Compound Recovery from Invasive Forest for Biofunctional Applications: Eucalyptus Species as a Case Study

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