Optimization of solid-phase microextraction conditions for gas chromatographic determination of ethanol and other volatile compounds in blood

Zuba, Dariusz; Parczewski, A.; Reichenbächer, Manfred

doi:10.1016/s1570-0232(02)00143-5

Cited by 54 publications

(39 citation statements)

References 7 publications

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“…The fibre-life seems mainly to be influenced by the number of samplings to which it has been submitted and by both the nature of the matrix under investigation and sampling conditions. As a consequence, reliable HS-SPME routine analysis of a high number of solid samples requires the fibre performance to be controlled at regular intervals with a reference material or solution and the results to normalised through correction factors such as the concentration factors versus static headspace of some of the target analytes [11] or the fibre concentration capability index (F ij ) [19,20] where the number of different fibre coatings compared must be replaced with that of periodical samplings.…”

Section: Discussionmentioning

confidence: 99%

Reliability of fibres in solid-phase microextraction for routine analysis of the headspace of aromatic and medicinal plants

Bicchi

Cordero

Liberto

et al. 2007

Journal of Chromatography A

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This article evaluates the HS-SPME recovery repeatability, intermediate precision and their performance over time when applied to HS-SPME sampling for quality control of medicinal and aromatic plants. Experiments were carried out on two sets of fibres coated with two different coatings and belonging to different lots (i.e 100 m polydimethylsyloxane (PDMS) and Carboxen/divinylbenzene/PDMS 50/30 m, l: 1 cm (CAR/DVB/PDMS)) and on chamomile (Matricaria chamomilla L.), sage (Salvia lavandulifolia Vahl.) and a standard solution containing 3-hexanol, isoamyl acetate, 1,8-cineole and menthol in diisobutyl phthalate. The performance of each set of fibres was evaluated by determining a group of complementary statistical parameters including: (i) repeatability of the absolute areas of each marker from each matrix with each fibre; (ii) intra-fibre repeatability of the total absolute areas of the markers of each matrix obtained with each fibre of each set; (iii) inter-fibre intermediate precision of the total absolute areas of the markers of each matrix obtained with all fibres of each set; and (iv) analysis of variance by one-way ANOVA with Fisher and Tukey tests. The influence of the number of analyses on fibre effectiveness (fibre life-time) was studied by linear regression analysis (LRA). The results proved that HS-SPME can successfully be used for routine control analysis of aromatic ad medicinal plants since both types of fibres showed good repeatability and intermediate precision of analytes recovery and consistency over time. Unlike data previously reported by other authors, CAR/DVB/PDMS coated fibres gave better results than those coated with PDMS. The fibre-life seemed mainly to be influenced by the number and conditions of samplings and nature of the matrix investigated.

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Section: Discussionmentioning

confidence: 99%

Reliability of fibres in solid-phase microextraction for routine analysis of the headspace of aromatic and medicinal plants

Bicchi

Cordero

Liberto

et al. 2007

Journal of Chromatography A

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“…By applying the salting out effect to HS-SPME, the signal intensity of the alcohols was improved. 31) However, the simultaneous volatile mixture extraction and the limited ber coating volume a ected the SPME extraction and volatiles having low interactions with the bers might be released from ber due to being more readily displaced. 36,37) erefore, low-molecular-weight volatiles tend to have lower area values while higher-molecularweight volatiles have improved area values (Fig.…”

Section: Comparison Of Headspace Micro Extraction Methodsmentioning

confidence: 99%

“…In addition, it is able to trap the sorbent with improved extraction e ciency for trace volatiles by combining techniques including cooling the sorbent and adding some salt to produce a salting out e ect. 31,32) e combination of these techniques would be expected to improve the sample volume loaded on the column and the sensitivity for the individual volatile components.…”

Section: Introductionmentioning

confidence: 99%

Multi-Component Profiling of Trace Volatiles in Blood by Gas Chromatography/Mass Spectrometry with Dynamic Headspace Extraction

et al. 2015

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A dynamic headspace extraction method (DHS) with high-pressure injection is described. is dynamic extraction method has superior sensitivity to solid phase micro extraction, SPME and is capable of extracting the entire gas phase by purging the headspace of a vial. Optimization of the DHS parameters resulted in a highly sensitive volatile pro ling system with the ability to detect various volatile components including alcohols at nanogram levels. e average LOD for a standard volatile mixture was 0.50 ng mL, and the average LOD for alcohols was 0.66 ng mL −1. is method was used for the analysis of volatile components from biological samples and compared with acute and chronic in ammation models. e method permitted the identi cation of volatiles with the same pro le pattern as in vitro oxidized lipid-derived volatiles. In addition, the concentration of alcohols and aldehydes from the acute in ammation model samples were signi cantly higher than that for the chronic in ammation model samples. e di erent pro les between these samples could also be identi ed by this method. Finally, it was possible to analyze alcohols and lowmolecular-weight volatiles that are di cult to analyze by SPME in high sensitivity and to show volatile proling based on multi-volatile simultaneous analysis.Please cite this article as: Mass Spectrom (Tokyo) 2015; 4(1): A0034

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“…The fiber performance was evaluated and confirmed through the fiber concentration capability index (Fij), first introduced by Zuba et al [15] and modified by Hamm et al [16] as: = where Fij is the concentration capability index of fiber j for analyte i, k is the number of fibers investigated and Hij is the peak height of analyte i with fiber j.…”

Section: +mentioning

confidence: 99%

Rapid Separation of Elemental Species by Fast Multicapillary Gas Chromatography with Multichannel Optical Spectrometry Detection following Headspace Solid Phase Microextraction

2015

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Abstract:A method for conducting fast and efficient gas chromatography based on short multicapillaries in straight alignment combined with atomic emission detection was developed for field analysis. The strategy enables for speciation analysis of organometallic compounds. The analytes are simultaneously ethylated and preconcentrated on a solid phase microextraction (SPME) fiber placed in the headspace over the sample for 25 min. The ethylated species are then completely separated and selectively quantified within 25 s under isothermal conditions. A new miniaturized speciation analyzer has been constructed and evaluated. The system consists of a GC injection port and a lab-made miniaturized GC unit directly coupled with miniaturized plasma excitation source. The emitted light is transferred via optical fiber and registered with a miniaturized charged coupled device (CCD) based spectrometer. Working parameters for multicapillary column gas chromatography with atomic emission detector, including carrier gas flow rate, desorption temperature, and GC column temperature, were optimized to achieve good separation of analytes. Basic investigations of the fundamental properties of 5 cm-long multicapillary column, to evaluate its potential and limitations as a rapid separation unit, are presented. The adaptation of the technique for use with a SPME system and with a multichannel element-selective plasmaemission detector is highlighted.

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Optimization of solid-phase microextraction conditions for gas chromatographic determination of ethanol and other volatile compounds in blood

Cited by 54 publications

References 7 publications

Reliability of fibres in solid-phase microextraction for routine analysis of the headspace of aromatic and medicinal plants

Reliability of fibres in solid-phase microextraction for routine analysis of the headspace of aromatic and medicinal plants

Multi-Component Profiling of Trace Volatiles in Blood by Gas Chromatography/Mass Spectrometry with Dynamic Headspace Extraction

Rapid Separation of Elemental Species by Fast Multicapillary Gas Chromatography with Multichannel Optical Spectrometry Detection following Headspace Solid Phase Microextraction

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