Optimization of Siderophore Production by Bacillus subtilis DR2 and Its Effect on Growth Promotion of Coriandrum sativum

Kumar, Pankaj; Kiran, Shilpi; Kumari, Shweta; Singh, Ajit

doi:10.3103/s1068367422060076

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…Urea also stimulated siderophore production in both isolates. Similar results were reported in siderophores produced from B. substilis DR2, which showed maximum production when using NaNO 3 as a nitrogen source [28]. The presence of yeast extract and urea stimulated the production of siderophores in P. aeruginosa [43].…”

Section: Discussionsupporting

confidence: 80%

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Optimization of Siderophore Production in Three Marine Bacterial Isolates along with Their Heavy-Metal Chelation and Seed Germination Potential Determination

Sarvepalli,

Velidandi,

Korrapati

2023

Microorganisms

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Siderophores are low-molecular-weight and high-affinity molecules produced by bacteria under iron-limited conditions. Due to the low iron (III) (Fe+3) levels in surface waters in the marine environment, microbes produce a variety of siderophores. In the current study, halophilic bacteria Bacillus taeanensis SMI_1, Enterobacter sp., AABM_9, and Pseudomonas mendocina AMPPS_5 were isolated from marine surface water of Kalinga beach, Bay of Bengal (Visakhapatnam, Andhra Pradesh, India) and were investigated for siderophore production using the Chrome Azurol S (CAS) assay. The effect of various production parameters was also studied. The optimum production of siderophores for SMI_1 was 93.57% siderophore units (SU) (after 48 h of incubation at 30 °C, pH 8, sucrose as carbon source, sodium nitrate as nitrogen source, 0.4% succinic acid), and for AABM_9, it was 87.18 %SU (after 36 h of incubation period at 30 °C, pH 8, in the presence of sucrose, ammonium sulfate, 0.4% succinic acid). The maximum production of siderophores for AMPPS_5 was 91.17 %SU (after 36 h of incubation at 35 °C, pH 8.5, glucose, ammonium sulfate, 0.4% citric acid). The bacterial isolates SMI_1, AABM_9, and AMPPS_5 showed siderophore production at low Fe+3 concentrations of 0.10 µM, 0.01 µM, and 0.01 µM, respectively. The SMI_1 (73.09 %SU) and AMPPS_5 (68.26 %SU) isolates showed siderophore production in the presence of Zn+2 (10 µM), whereas AABM_9 (50.4 %SU) exhibited siderophore production in the presence of Cu+2 (10 µM). Additionally, these bacterial isolates showed better heavy-metal chelation ability and rapid development in seed germination experiments. Based on these results, the isolates of marine-derived bacteria effectively produced the maximum amount of siderophores, which could be employed in a variety of industrial and environmental applications.

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Section: Discussionsupporting

confidence: 80%

“…All the isolated colonies were sub-cultured repeatedly on ZMA to obtain pure cultures. The obtained pure cultures were stored at 4 • C, and 30% glycerol stocks were prepared and preserved at −20 • C for future use [28].…”

Section: Isolation and Screening Of Siderophore-producing Marine Bact...mentioning

confidence: 99%

Optimization of Siderophore Production in Three Marine Bacterial Isolates along with Their Heavy-Metal Chelation and Seed Germination Potential Determination

Sarvepalli,

Velidandi,

Korrapati

2023

Microorganisms

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“…Various beneficial plant-associated bacterial species produce this type of siderophore, such as 2,3-dihydroxybenzoylglycine ( Bacillus subtilis ) [ 24 ], spirilobactin ( Azospirillum brasilense ), 2,3-dihydroxybenzoic acid ( Azospirillumlipoferum) [ 25 ], aminocholine, nitrocholine, protochelin ( Azotobacter vinelandii ) [ 26 ], Agrobactin ( Rhizobium radiobactor ) [ 27 ], and 2,3-dihydroxybenzoic acid conjugated to threonine ( Rhizobium leguminosarum ) [ 28 ]. For example, the catecholate siderophore produced by Bacillus subtilis is reported to enhance seed germination and plant growth in Coriandrum sativum [ 29 ]. Under drought conditions, catecholate is reported to enhance soybean seed germination [ 30 ].…”

Section: Microbial Exudates and Their Compositionmentioning

confidence: 99%

Microbial Exudates as Biostimulants: Role in Plant Growth Promotion and Stress Mitigation

Ansari,

Devi,

Sarkar

et al. 2023

JoX

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Microbes hold immense potential, based on the fact that they are widely acknowledged for their role in mitigating the detrimental impacts of chemical fertilizers and pesticides, which were extensively employed during the Green Revolution era. The consequence of this extensive use has been the degradation of agricultural land, soil health and fertility deterioration, and a decline in crop quality. Despite the existence of environmentally friendly and sustainable alternatives, microbial bioinoculants encounter numerous challenges in real-world agricultural settings. These challenges include harsh environmental conditions like unfavorable soil pH, temperature extremes, and nutrient imbalances, as well as stiff competition with native microbial species and host plant specificity. Moreover, obstacles spanning from large-scale production to commercialization persist. Therefore, substantial efforts are underway to identify superior solutions that can foster a sustainable and eco-conscious agricultural system. In this context, attention has shifted towards the utilization of cell-free microbial exudates as opposed to traditional microbial inoculants. Microbial exudates refer to the diverse array of cellular metabolites secreted by microbial cells. These metabolites enclose a wide range of chemical compounds, including sugars, organic acids, amino acids, peptides, siderophores, volatiles, and more. The composition and function of these compounds in exudates can vary considerably, depending on the specific microbial strains and prevailing environmental conditions. Remarkably, they possess the capability to modulate and influence various plant physiological processes, thereby inducing tolerance to both biotic and abiotic stresses. Furthermore, these exudates facilitate plant growth and aid in the remediation of environmental pollutants such as chemicals and heavy metals in agroecosystems. Much like live microbes, when applied, these exudates actively participate in the phyllosphere and rhizosphere, engaging in continuous interactions with plants and plant-associated microbes. Consequently, they play a pivotal role in reshaping the microbiome. The biostimulant properties exhibited by these exudates position them as promising biological components for fostering cleaner and more sustainable agricultural systems.

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“…Some endophytic bacteria live inside the tissues of plants and help in nutrient acquisition. For example, Bacillus subtilis can produce siderophores that chelate iron, making it available to the plant [ 32 ]. Other endophytic bacteria can produce plant growth-promoting hormones, such as indole acetic acid (IAA), stimulating root growth and nutrient uptake.…”

Section: Endophytic Microorganisms In Plantmentioning

confidence: 99%

Endosphere microbial communities and plant nutrient acquisition toward sustainable agriculture

Babalola,

Adedayo

2023

Emerging Topics in Life Sciences

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Endophytic microbial communities have essential information for scientists based on their biological contribution to agricultural practices. In the external plant environment, biotic and abiotic factors affect microbial populations before getting into plant tissues. Endophytes are involved in mutualistic and antagonistic activities with the host plant. Microbial communities inhabiting the internal tissues of plant roots depend on their ability to live and contend with other plant microflora. The advantageous ones contribute to soil health and plant growth either directly or indirectly. The microbial communities move via soil–root environment into the endosphere of plants promoting plant growth features like antibiosis, induced systemic resistance, phytohormone synthesis, and bioremediation. Therefore, the existence of these microorganisms contributes to plant genomes, nutrient availability in the soil, the presence of pathogens, and abiotic factors. This review aims at how endophytic microorganisms have displayed great interest in contributing to abundant crop production and phytopathogen inhibition.

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Optimization of Siderophore Production by Bacillus subtilis DR2 and Its Effect on Growth Promotion of Coriandrum sativum

Cited by 4 publications

References 20 publications

Optimization of Siderophore Production in Three Marine Bacterial Isolates along with Their Heavy-Metal Chelation and Seed Germination Potential Determination

Optimization of Siderophore Production in Three Marine Bacterial Isolates along with Their Heavy-Metal Chelation and Seed Germination Potential Determination

Microbial Exudates as Biostimulants: Role in Plant Growth Promotion and Stress Mitigation

Endosphere microbial communities and plant nutrient acquisition toward sustainable agriculture

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