Optimization of separation conditions in the size-selective capillary electrophoretic separation of DNA fragments

Chemometrics in capillary electrophoresis. Part A: Methods for optimization

“…In this case, the experimental variables studied were background electrolyte concentration, pH, voltage, SDS concentration, and methanol percentage. The separation of DNA fragments by size-selective CE [12] and absorbing species in human urine [19] were also optimized by simplex.…”

Experimental design as a tool when evaluating stationary phases for the capillary electrochromatographic separation of basic peptides

“…The basic requirements were that these response functions should provide measures of baseline separation and peak breadth for the four peptides. In addition, three chromatographic response functions were utilized: the chromatographic optimisation function, COF [25,26], the chromatographic response function, CRF 3 [26][27][28][29], and the resolution product, R p [30].…”

“…Many different chromatographic functions have been described in the literature [25][26][27][28][29][30][31][32][33][34], however, only the chromatographic optimisation function, COF, the chromatographic response function, CRF 3 (modified according to [29]), and the resolution product, R p were used here. The chromatographic functions were calculated according to equations (3)-(5):…”

“…The weighting factors, a (the excess resolution factor) and b (the overlap degradation factor), were selected according to [28,29], and set to 5 and 50, respectively. This procedure gives more weight to the resolution (second) term.…”

Simplex optimization of electrokinetic injection of DNA in capillary electrophoresis using dilute polymer solution

“…However, chemometric methods are particularly suitable to optimize separation conditions in both CE [23][24][25] and chromatography [26,27]. Sizing separation of DNA fragments in polymer solutions has been optimized for column length and methylcellulose concentration [28], however, injection-related aspects have not been addressed. In our work, the influence of different buffer concentrations of the DNA samples during EK injection was evaluated in a systematic series of experi-ments.…”