Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis

Abstract

Saccharification potential of xylanase enzyme cloned from Bacillus licheniformis into E. coli BL21 (DE3) was evaluated against plant biomass for the production of bioethanol. The expression of cloned gene was studied and conditions were optimized for its large scale production. The parameters effecting enzyme production were examined in a fermenter. Recombinant xylanase has the ability to breakdown birchwood xylan to release xylose as well as the potential to treat plant biomass, such as wheat straw, rice straw, and sugarcane bagass. The saccharification ability of this enzyme was optimized by studying various parameters. The maximum saccharification percentage (84%) was achieved when 20 units of recombinant xylanase were used with 8% sugarcane bagass at 50°C and 120 rpm after 6 hours of incubation. The results indicated that the bioconversion of natural biomass by recombinant xylanase into simple sugars can be used for biofuel (bioethanol) production. This process can replace the use of fossil fuels, and the use of bioethanol can significantly reduce the emission of toxic gases. Future directions regarding pre-treatment of cellulosic and hemicellulosic biomass and other processes that can reduce the cost and enhance the yield of biofuels are briefly discussed.

Xylanase gene mutation by error-prone pcr and expression in Pichia pastoris

“…The reason of the difference may be due to the different enzyme resources, xylanase purity or the modified xylanase gene (PUCHART et al, 2018). It was reported that xylanase gene from Bacillus licheniformis was expressed in E.coli, the enzyme was effective for sugarcane bagasse saccharification (AFTAB et al, 2018), in agreement with this study. In addition, the xylanase from Aspergillus niger could improve the yields of glucose and arabinose effectively (p<0.05), which may be due to the xylanase compound isolated from Aspergillus niger in this study contains xylanase as well as other enzymes.…”

Enzymatic hydrolysis of low temperature alkali pretreated wheat straw using immobilized β-xylanase nanoparticles

“…In another study, maximum saccharification potential was checked against sugarcane bagasse using the recombinant xylanase enzyme. 68 The saccharification efficiency obtained here be compared and discussed with saccharification efficiencies measured by other researchers are enlisted in Table 4.…”

‘The sadness of goodbye in a funny movie’: Desiree Akhavan’s Appropriate Behavior and the melancholic legacy of Annie Hall in contemporary US film and television break-up narratives

“…But there is an infinite pool for men to play in. (Akhavan quoted in Aftab 2015) For Akhavan, these comparisons play a part in diminishing the value of her work to film culture. She also positions this in relative terms alongside the way that the work of her male peers has been seen and treated (she is certainly correct that hers is far from the only work to emerge from recent American independent cinema that bears obvious comparison with Allen's).…”