Optimization of rhamnolipid production by Pseudomonas aeruginosa OG1 using waste frying oil and chicken feather peptone

Abstract

In the present study, production of rhamnolipid biosurfactant by Pseudomonas aeruginosa OG1 was statistically optimized by response surface methodology. Box-Behnken design was applied to determine the optimal concentrations of 52, 9.2, and 4.5 g/L for carbon source (waste frying oil), nitrogen source (chicken feather peptone), and KH 2 PO 4 , respectively, in production medium. Under the optimized cultivation conditions, rhamnolipid production reached up to 13.31 g/L (with an emulsification activity of 80%), which is approximately twofold higher than the yield obtained from preliminary cultivations. Hence, rhamnolipid production, noteworthy in the literature, was achieved with the use of statistical optimization on inexpensive waste materials for the first time in the present study.

Use of Chicken Feather Peptone and Sugar Beet Molasses as Low Cost Substrates for Xanthan Production by Xanthomonas campestris MO-03

“…Others have reported that some organic nitrogen sources such as yeast extract [29], ram horn peptone [30], and peptone-tyrptone [28] contribute to the formation of xanthan gum. The results of our previous studies [19,21,23] showed that CFP contains all of these amino acids (alanine, cysteine, glutamate, and proline) at varying concentrations. For the production of xanthan gum, X. campestris requires some micro-(e.g., K, Fe, P, Mg, S and Ca salts) and macronutrients (C and N) [31,32].…”

“…Feathers constitute up to 10% of total chicken weight. They consist of approximately 90% protein composed of keratin, and thus can be a potential source of proteins and amino acids [19][20][21]. Considering these characteristics of the feathers, they are cheap and abundant for peptone production.…”

“…Peptones are the hydrolysis products of nitrogen rich substrates. Recently, the chicken feather peptone has been used as a complex nitrogen source for the production of rhamnolipid [19], citric acid [21], and polyhydroxyalkanoate (bioplastic) [22].…”

Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

“…For instance, compared to water-soluble carbon sources (e.g., glucose), the use of water-insoluble carbon such as vegetable oil generally produces rhamnolipids in higher titers (Chong and Li 2017 ). The use of factorial design to optimize culture conditions has been proven to be a successful approach to significantly increase the rhamnolipid yield obtained using several P. aeruginosa strains (Camilios-Neto et al 2008 ; Kumar et al 2015 ; Ozdal et al 2017 ; El-Housseiny et al 2019 ). Various studies (see Table 1 ) have explored economical production methods using low-cost and readily available nutrients.…”

“…To develop a cost-effective bioprocess, cheap nutrient sources are needed and complex mineral medium cannot be used. The use of domestic or industrial wastes for rhamnolipid production is both cost-effective and environmental-friendly (Ozdal et al 2017 ).…”