Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

Kaisto, Susanna; Saarela, Ulla; Dönges, Laura; Raykhel, Irina; Skovorodkin, Ilya; Vainio, Seppo

doi:10.3791/60995-v

Cited by 3 publications

(3 citation statements)

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“…Therefore, models including endothelial cells and blood-like perfusion would be very useful, particularly to study podocyte foot effacement and glomerular basement membrane thickening. The glomeruli produced in vitro in organoids can be vascularized upon in vivo transplantation or chicken chorioallantoic membrane engraftment [169][170][171] . While a few endothelial cells were co-developed in the early kidney organoids produced around 2015 (ref.…”

Section: Nephropathiesmentioning

confidence: 99%

Towards a better understanding of diabetes mellitus using organoid models

2023

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Our understanding of diabetes mellitus has benefited from a combination of clinical investigations and work in model organisms and cell lines. Organoid models for a wide range of tissues are emerging as an additional tool enabling the study of diabetes mellitus. The applications for organoid models include studying human pancreatic cell development, pancreatic physiology, the response of target organs to pancreatic hormones and how glucose toxicity can affect tissues such as the blood vessels, retina, kidney and nerves. Organoids can be derived from human tissue cells or pluripotent stem cells and enable the production of human cell assemblies mimicking human organs. Many organ mimics relevant to diabetes mellitus are already available, but only a few relevant studies have been performed. We discuss the models that have been developed for the pancreas, liver, kidney, nerves and vasculature, how they complement other models, and their limitations. In addition, as diabetes mellitus is a multi-organ disease, we highlight how a merger between the organoid and bioengineering fields will provide integrative models.

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Section: Nephropathiesmentioning

confidence: 99%

Towards a better understanding of diabetes mellitus using organoid models

2023

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“…A connection to the chick´s circulating system was also observed by Schmidt et al while cultivating blood vessel organoids in the CAM model [62]. The recovery of tubulogenic capacity was shown by Kaisto et al, as kidney spheroids were grafted onto the CAM [63]. In future projects, organoids obtained from pancreatic cancer could be cultured on the CAM to test chemotherapeutic regimens.…”

Section: Organoids and Cam Modelmentioning

confidence: 68%

Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing

Ettner-Sitter,

Montagner,

Kuenzel

et al. 2024

Organoids

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Although significant improvements have been made in the treatment of pancreatic cancer, its prognosis remains poor with an overall 5-year survival rate of less than 10%. New experimental approaches are necessary to develop novel therapeutics. In this study, the investigation of pancreatic cancer tissue growth in the chorioallantoic membrane (CAM) model and the subsequent use of indocyanine green (ICG) injections for the verification of intratumoral perfusion was conducted. ICG was injected into the CAM vasculature to visualize the perfusion of the tumor tissue. The presence of metastasis was investigated through PCR for the human-specific ALU element in the liver of the chicken embryo. Additionally, the usage of cryopreserved pancreatic tumors was established. Intratumoral perfusion of tumor tissue on the CAM was observed in recently obtained and cryopreserved tumors. ALU-PCR detected metastasis in the chick embryos’ livers. After cryopreservation, the tissue was still vital, and the xenografts generated from these tumors resembled the histological features of the primary tumor. This methodology represents the proof of principle for intravenous drug testing of pancreatic cancer in the CAM model. The cryopreserved tumors can be used for testing novel therapeutics and can be integrated into the molecular tumor board, facilitating personalized tumor treatment.

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“…Other studies report how CAM can also be used for transplanting sections of organs or organoids from murine embryos, such as, for example, the kidney. Embryonic kidneys were taken at ED11.5, transplanted onto CAM at ED8, and then cultivated for 7 days; subsequently, the murine embryonic kidney, grown on CAM, underwent immunohistochemistry for endothelial growth markers, highlighting the anastomosis between the blood vessels of CAM and those of the murine kidney [192].…”

Section: Use Of Cam To Set-up Organotypic Culturementioning

confidence: 99%

CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing

Palumbo,

Sisi,

Checchi

2023

Biology

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We are witnessing the revival of the CAM model, which has already used been in the past by several researchers studying angiogenesis and anti-cancer drugs and now offers a refined model to fill, in the translational meaning, the gap between in vitro and in vivo studies. It can be used for a wide range of purposes, from testing cytotoxicity, pharmacokinetics, tumorigenesis, and invasion to the action mechanisms of molecules and validation of new materials from tissue engineering research. The CAM model is easy to use, with a fast outcome, and makes experimental research more sustainable since it allows us to replace, reduce, and refine pre-clinical experimentation (“3Rs” rules). This review aims to highlight some unique potential that the CAM-assay presents; in particular, the authors intend to use the CAM model in the future to verify, in a microenvironment comparable to in vivo conditions, albeit simplified, the angiogenic ability of functionalized 3D constructs to be used in regenerative medicine strategies in the recovery of skeletal injuries of critical size (CSD) that do not repair spontaneously. For this purpose, organotypic cultures will be planned on several CAMs set up in temporal sequences, and a sort of organ model for assessing CSD will be utilized in the CAM bioreactor rather than in vivo.

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Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

Cited by 3 publications

References 0 publications

Towards a better understanding of diabetes mellitus using organoid models

Towards a better understanding of diabetes mellitus using organoid models

Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing

CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing

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