Optimization of recombinant vaccine antigen dose in mouse model by ELISA and immunophenotyping

Bs, Reddy; Hms, Kumar

doi:10.15761/gvi.1000123

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2020

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“…For the active immunization assays, mice were immunized subcutaneously with 10-μg AgBR1 [ 16 , 17 , 18 , 19 , 20 ] with aluminum hydroxide (AH-AgBR1) or aluminum hydroxide alone (AH) boosted twice every two weeks with the same amount of AH-AgBR1 or AH (1:1 AH-AgBR1 or AH with sterilized PBS, 300ul per dose). Two weeks after the final immunization, the mice were anaesthetized with ketamine-xylazine and challenged by ZIKV-infected mosquitoes.…”

Section: Methodsmentioning

confidence: 99%

Vaccination with Aedes aegypti AgBR1 Delays Lethal Mosquito-Borne Zika Virus Infection in Mice

et al. 2020

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Zika Virus (ZIKV) is transmitted primarily by Aedes aegypti mosquitoes, resulting in asymptomatic infection, or acute illness with a fever and headache, or neurological complications, such as Guillain-Barre syndrome or fetal microcephaly. Previously, we determined that AgBR1, a mosquito salivary protein, induces inflammatory responses at the bite site, and that passive immunization with AgBR1 antiserum influences mosquito-transmitted ZIKV infection. Here, we show that the active immunization of mice with AgBR1 adjuvanted with aluminum hydroxide delays lethal mosquito-borne ZIKV infection, suggesting that AgBR1 may be used as part of a vaccine to combat ZIKV.

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