Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli

Yavuz, Elif; Güneş, Hatice; Harsa, Şebnem; Bulut, Çisem; Yenidünya, Ali Fazıl

doi:10.1007/s11274-004-1004-3

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…One aspect of this diversity may be explained by the dynamic repertoire of plasmids found in the B. cereus group as well as in individual strains of the B. cereussensu stricto (Rasko et al 2004; Rasko et al 2005; Rasko et al 2007).We determined the relationships between the B. cereus strain, isolated from clinical, food and water sources using PFGE analysis using SmaI restriction endonuclease(Akamatsu et al 2019;Liu et al 2016;Yavuz et al 2004). The ngerprints generated by macrorestriction of the DNA comprised approximately 10 to 23 bands of 48.5 to 727.5 kb.…”

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Genetic Diversity and The Presence of Circular Plasmids in Bacillus Cereus Isolates of Clinical and Food Origin

Kišek

Pogačnik

Torkar

2021

Preprint

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The diversity of 61 Bacillus cereus strains isolated from different clinical specimens, food including raw milk and milk products, and water was evaluated. PFGE analysis could discriminate 61 distinct pulsotypes with similarity levels from 25 to 82%, which were divided into 13 clonal complexes. The similarity between clonal complexes was at least 40%. Clinical strains were divided into 10 clonal complexes, while the strains, isolated from milk, food and water were included in 9, 6 and 6 clonal complexes, respectively. Three clonal complexes were dominated by clinical isolates, while they were absent in two complexes. Bacterial isolates from foods, being a probable source of alimentary toxoinfection, showed low similarity to isolates from stool specimens. The isolates from both sources were classified together in only 4 out of 13 clonal complexes. The large circular and linear plasmids with the sizes between 50 and 200 kb were detected in 24 (39.3%) and 14 (23%) B. cereus strains, respectively. Thirteen (21.3%) strains contained only one plasmid, two plasmids were found in 6 (9.8%) of strains, and three or more plasmids were obtained in 5 (8.2%) of tested strains. The plasmids were confirmed in 30.8% and 40% of isolates from clinical specimens and food and milk samples, respectively. No clear correlation between the PFGE profiles, the source as well as plasmid content among all tested strains was observed.

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confidence: 99%

Genetic Diversity and The Presence of Circular Plasmids in Bacillus Cereus Isolates of Clinical and Food Origin

Kišek

Pogačnik

Torkar

2021

Preprint

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Genetic diversity and the presence of circular plasmids in Bacillus cereus isolates of clinical and environmental origin

2021

View full text Add to dashboard Cite

The diversity of 61 Bacillus cereus strains isolated from different clinical specimens, food including raw milk and milk products, and water was evaluated. PFGE analysis could discriminate 61 distinct pulsotypes with similarity levels from 25 to 82%, which were divided into 13 clonal complexes. The similarity between clonal complexes was at least 40%. Clinical strains were divided into 10 clonal complexes, while the strains, isolated from milk, food and water were included in 9, 6 and 6 clonal complexes, respectively. Three clonal complexes were dominated by clinical isolates, while they were absent in two complexes. Bacterial isolates from foods, being a probable source of alimentary toxoinfection, showed low similarity to isolates from stool specimens. The isolates from both sources were classi ed together in only 4 out of 13 clonal complexes. The large circular and linear plasmids with the sizes between 50 and 200 kb were detected in 24 (39.3%) and 14 (23%) B. cereus strains, respectively. Thirteen (21.3%) strains contained only one plasmid, two plasmids were found in 6 (9.8%) of strains, and three or more plasmids were obtained in 5 (8.2%) of tested strains. The plasmids were con rmed in 30.8% and 40% of isolates from clinical specimens and food and milk samples, respectively. No clear correlation between the PFGE pro les, the source as well as plasmid content among all tested strains was observed.

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A novel strain of Bacillus amyloliquefaciens displaying broad spectrum antifungal activity and its underlying mechanism

2015

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Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli

Cited by 3 publications

References 8 publications

Genetic Diversity and The Presence of Circular Plasmids in Bacillus Cereus Isolates of Clinical and Food Origin

Genetic Diversity and The Presence of Circular Plasmids in Bacillus Cereus Isolates of Clinical and Food Origin

Genetic diversity and the presence of circular plasmids in Bacillus cereus isolates of clinical and environmental origin

A novel strain of Bacillus amyloliquefaciens displaying broad spectrum antifungal activity and its underlying mechanism

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