Optimization of Pulsed Electric Fields-Assisted Extraction of Polyphenols from Potato Peels Using Response Surface Methodology

Abstract

In this work, optimal Pulsed Electric Fields-assisted extraction conditions were selected in order to intensify the extractability of polyphenol compounds with high antioxidant activity from potato peels.Effectiveness of PEF as cell disintegration technique was confirmed using both impedance measurements and Scanning Electron Microscopy (SEM). Solid-liquid extraction (SLE) for both untreated and PEF pre-treated potato peels was optimized to determine the most effective solvent concentration (0-100% ethanol in water) as well as extraction temperature (20-50°C) and time (30-240 min) using response surface methodology. Total phenolic compounds (TPC) and antioxidant activity (DPPH) of the extracts were determined. Results showed that the application of PEF prior to SLE has the potential to reduce duration, temperature and consumption of solvent to achieve the same recovery yield of phenolic compounds. Under optimized conditions (54% ethanol, 233 min and 50°C for SLE; 52% ethanol, 230 min and 50°C for PEF), the extracts obtained from PEF pre-treated samples showed higher total phenolics yield (10%) and antioxidant activity (9%) as compared to the control extraction. Finally, HPLC-DAD analysis revealed the major classes of the detected polyphenolic compounds as chlorogenic, caffeic, syringic, protocatechuic and p-coumaric acids, and no significant degradation of individual polyphenols due to PEF application was observed.

Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products

“…Besides OH, pulsed electric fields (PEF) could also be an interesting option to explore, as it acts by destroying the cell wall and plant tissue structure and facilitating extraction. Though to our knowledge it has never been applied to pine matrices, it was successfully applied in the extraction of different compounds from many agri-food wastes (e.g., Norway spruce bark, tomato, and potato peels, among others) [131][132][133].…”

Plum ( Prunus domestica L.) leaves extract as a natural antioxidant: Extraction process optimization and sunflower oil oxidative stability evaluation

“…Higher EtOH% induce protein denaturation which in turn decreased the dissolution of polyphenols leading to low TPC yield (Dahmoune et al., 2015). Furthermore, the low TPC yield at high EtOH% might be ascribed to the effect of ethanol on barrier properties of the plant cell membrane (Frontuto et al., 2019).…”

The application of PEF technology in food processing and human nutrition

“…The concentration of extracted total phenolic compounds doubled when tomato waste was treated with a 2 kV/cm and 700 pulses. Other exemplary studies concern the application of PEF for enhancing the extraction of polyphenols from lemon peel residues (Peiró et al 2019 ), potato peels (Frontuto et al 2019 ), mango and papaya by-products (Parniakov et al 2016b ).…”