Optimization of proteinase K incubation protocol duration during DNA extraction from oral squamous cell carcinoma FFPE samples
Asti Meizarini,
Astari Puteri,
Yanna Debby Restifanny Yasan
et al.
Abstract:Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented. Thus, it is necessary to optimize the FFPE DNA extraction protocol to obtain the best results. Proteinase K incubation is undoubtedly crucial in DNA extraction procedures, but this step is often not well explained in the manufacturer’s manual. Purpose: This study aimed to find… Show more
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