Optimization of protease activity of alkaliphilic bacteria isolated from an alkaline lake in India

Kanekar, Pradnya Pralhad; Nilegaonkar, Smita; Sarnaik, Seema; Kelkar, A. S.

doi:10.1016/s0960-8524(02)00018-4

Cited by 102 publications

(54 citation statements)

References 10 publications

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“…Therefore, L21 was thought to produce less side by-products than the other two strains. An inoculation ratio of 2-5% was an optimum value for Bacillus type of strains reported in the literature, which supported our findings [14,18].…”

Section: Effect Of Inoculation Ratiosupporting

confidence: 92%

Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats

Gençkal

Tarı

2006

Enzyme and Microbial Technology

124

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Bacillus strains isolated under extreme alkaline conditions (Izmir, Turkey), were screened and identified for high alkaline protease activity. Strains with high protease yields were optimized with respect to inoculum concentration, temperature, agitation speed, initial medium pH and incubation time. Three Bacillus strains coded as I18, L18 and L21 showed high potential, for alkaline protease activity (160-222 U/ml) among 85 isolates. The specific growth rates were estimated from the growth curves as 0.49 h −1 for I18, as 0.6 and 0.7 h −1 for L18 and L21, respectively. The optimum temperatures were determined as 30 • C for strain I18 and 37 • C for the strains L18 and L21. Similarly, the optimum agitation speeds were 100 rpm for I18 and 180 rpm for L18 and L21. For all three strains, the optimum inoculation ratio and incubation time, were determined as 5% (v/v) and 96 h, respectively. The optimum initial media pH was found as pH 10 for strain L18 and L21. Bacillus sp. L21 with the highest specific protease activity (60 U/mg protein) and a broader pH range was chosen for further study. The biomass and product yield for this strain was determined as 0.023 g cell/g glucose and 0.021 U/g glucose, respectively. The crude enzyme of this strain was further characterized and was determined as a bleach stable, serine alkaline protease with an optimum temperature of 60 • C and a pH of 11, with a potential to be a candidate for the applications in the detergent industry.

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Section: Effect Of Inoculation Ratiosupporting

confidence: 92%

Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats

Gençkal

Tarı

2006

Enzyme and Microbial Technology

124

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“…In view of the above realization, exploration of extremophiles and their biocatalytic machinery has been attracting the greater attention. Among the various groups of extremophiles, halo-tolerant/halophilic organisms are the major categories with greater biocatalytic potential (17,32).…”

Section: Introductionmentioning

confidence: 99%

Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1

Thumar

Singh

2007

Braz. J. Microbiol.

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An alkaliphilic and salt-tolerant actinomycete, Streptomyces clavuligerus strain Mit-1, was isolated from Mithapur, the western coast of India. The organism was Gram-positive, having filamentous, long thread like structure. The sporulation started after two days of growth and the optimum level of alkaline protease (130 U/ ml) was produced during the early stationary phase. The strain could grow and produce protease with 0-10% NaCl (w/v), the optimum being 5% NaCl (w/v). Growth and protease production was optimum at pH 9 with substantial decline at neutral pH. Sucrose and gelatin were the best carbon and nitrogen sources respectively, whereas gelatin broth was the preferred medium for protease production. Mit-1 produced substantial protease with various amino acids, when employed as the sole nitrogen sources. Crude substrates, such as molasses, whey and wheat flour had significant effect on enzyme production. The results are quite valuable, as only few actinomycetes, particularly salt-tolerant alkaliphilic ones, have so far been explored for their enzymatic potential and process optimization.

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“…On the other hand, other carbon sources were reported for maximal protease production based on the source organism (JOSHI et al, 2008;SHIVANAND;JAYARAMAN, 2009;JAYAKUMAR et al, 2012). Less production of alkaline protease by B. agaradhaerens strain AK-R in the presence of simple sugar like glucose, galactose, sucrose, and maltose is mostly due to catabolite repression of protein biosynthesis (KANEKAR et al, 2002;DENG et al, 2010). …”

Section: Effect Of Carbon Sourcementioning

confidence: 99%

Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain ak-r isolated from egyptian soda lakes

Ibrahim

Al‐Salamah

Elbadawi³

et al. 2016

Biosci. J.

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Alkaline proteases are hydrolytic enzymes that cleave peptide bonds in proteins and peptides in alkaline conditions, which occupy a pivotal importance with respect to their industrial applications. This study aimed to isolate new alkaline protease producing alkaliphilic bacteria from Egyptian soda lakes and optimize the fermentation process to enhance the enzyme production. The extensive screening process of the samples collected from Egyptian soda lakes resulted in isolation of a potent alkaline protease producing alkaliphilic strain AK-R. The isolate was identified as Bacillus agaradhaerens strain AK-R based on 16S rRNA gene analysis (99%). Wheat bran and gelatin supported maximum alkaline protease production as carbon and nitrogen sources, respectively. Strain AK-R is halo-tolerant thermotolerant alkaliphilic bacterium in nature, as it can grow over a wide range of NaCl concentrations (up to 25%) and up to 55 °C, with maximal growth and enzyme production at 2.5-5%, and pH 11 at 35 °C. Among the tested cations, only Mg 2+ and Ca 2+ ions significantly enhanced the enzyme production by about 1.2, and 1.3 fold compared to control, respectively. Alkaline protease secretion was coherent with the growth pattern, reaching maximal yield after about 32 h (mid stationary phase). In conclusion a new halo-tolerant thermo-tolerant alkaliphilic alkaline protease producing Bacillus agaradhaerens strain AK-R was isolated from Egyptian soda lakes. Optimization of the nutritional and cultivation conditions resulted in increase of enzyme yield by 20 fold. Strain AK-R and its extracellular alkaline protease with salt, pH and temperature, tolerance signify their potential application in laundry and pharmaceuticals industries.

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Optimization of protease activity of alkaliphilic bacteria isolated from an alkaline lake in India

Cited by 102 publications

References 10 publications

Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats

Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats

Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1

Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain ak-r isolated from egyptian soda lakes

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