Optimization of process parameters for efficient decolorization of Congo red by novel fungus<i>Curvularia</i>sp. using Box–Behnken design

Senthilkumar, S.; Perumalsamy, Muthiah; Prabhu, H. J.; Thajudin, Nooruddin

doi:10.1080/19443994.2014.889437

Cited by 3 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The variance analysis of the linear regression model showed that the model had high significance and that the p ‐value was very low ( p < 0.0001). Lack of fit, models failed to represent experimental data in the experimental field at points not included in regression analysis . A lack of fit was found to be nonsignificant ( p = 0.0736), indicating that the model equation was sufficient to predict xylanase activity under any combination of the variables.…”

Section: Resultsmentioning

confidence: 97%

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

View full text Add to dashboard Cite

Xylanase is a member of an important family of enzymes that has been used in many biotechnological processes. However, the overall cost of enzyme production has been the main problem in the industrial application of enzymes. To obtain maximum xylanase production, statistical approaches based on the Plackett-Burman design and response surface methodology were employed. The results of the statistical analyses demonstrated that the optimal conditions for increased xylanase production were the following: inoculum size, 3.8%; maize meal, 4.5%; histidine, 0.6%; methanol, 1%; culture volume, 20%; bean pulp, 30 g L −1 ; and Tween-80, 0.8%; and pH 5.0. Verification of the optimization demonstrated that 3273 U mL −1 xylanase was observed under the optimal conditions in shake flask experiments. SDS-PAGE results showed that the size of xylanase protein was about 23 kDa. The results showed that the xylanase produced by fermentation came from Aspergillus Niger by MALDI-TOF-MS. The optimized medium resulted in 2.1-and 1.4-fold higher the activity of xylanase compared with the unoptimized medium (the main nutrients are maize meal and bean pulp) and laboratory medium (the main nutrients are yeast extract and peptone), respectively.The optimization of fermentation conditions is an effective means to reduce production cost and improve xylanase activity. K E Y W O R D Smethodology optimization, Plackett-Burman, xylanase Abbreviations: PBS, phosphate-buffered saline; RSM, response surface method.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

Section: Resultsmentioning

confidence: 97%

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

View full text Add to dashboard Cite

show abstract

“…Biomass plays an important role in the decolorisation of the dyes, since it enhances the degradation of the dyes through increasing the activity of the enzyme. Cell biomass has been suggested as a promising way to remove the color through bio sorption [24]. In the present study Congo red was tested for 5 days to determine the ability of fungus Termitomyces sp.…”

Section: Effect Of Termitomyces Sp On the Decolorisation Of Congo Redmentioning

confidence: 99%

“…[22] Phanerochaete chrysosporium [23], Caldariomyces fumago [24], Curvularia sp. [25] and Trametes versicolor [26] were reported earlier as Congo red degrading fungi.…”

Section: Introductionmentioning

confidence: 99%

Decolorisation of Azo Dye Congo Red (CR) by Termitomyces sp. Biomass

Jackson

Gomathi

2021

IJECC

View full text Add to dashboard Cite

Aims: A study was conducted to evaluate decoloration of azo dye, Congo Red (CR) using fungal hyphal mat of beneficial bacidiomycete Termitomyces sp. TMS7 (MW694830) as bio sorbent material. Study design: Completely randomized block design (CRD). Place and duration of study: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India, between September 2019 and January 2020. Methodology: Isolation of white rot fungus from basidiocarb was done and screened based on their ligninolytic enzyme activity and Isolate TMS 7 was selected as best isolate and identified through ITS 1 and ITS 4 primers. Efficiency of fungal biomass to decolorize Congo red was assessed and per cent decoloration and kinetics were calculated. Results: Twelve fungal isolates were obtained and Isolate TMS 7 was selected as best isolate based on enzymatic activity. TMS 7 was identified as Termitomyces sp. using ITS 1 and ITS 4 primer. Ligninolytic enzymes i.e. cellulase (9.97 µ mol of glucose released/min/mg protein), and xylanase (9.55 µ mol of xylose released/min/mg protein) were quantified from the crude fungal extract of TMS 7, which was higher than standard (Termitomyces albuminosus -MTCC 1366). Decolorisation efficiency of termitomyces fungal biomass (1 g/100 ml) against different concentration of congo red dye (50-250 mg/L) was assessed. About 100 % (99.9) degradation was recorded in the minimum dye concentration of 50 mg/L within 3 days and 8 % decoloration was achieved at the highest dye concentration (250 mg/L) within 5 days. Conclusion: Possible mechanism of degradation is the presence of lignolytic enzyme especially cellulase, xylanase in the culture filtrate and bio sorption of degraded product by the fungal cell wall components viz., chitin, glucan other complex polymers.

show abstract

“…Microorganisms from the genus Curvularia are microscopic fungi most often isolated from the soil, although they can also be isolated from plant and animal tissues [ 9 , 10 ]. Strains from this genus are able to undergo steroid transformation, produce compounds with antibacterial and antifungal activity, and degrade polyaromatic hydrocarbons, dyes, and polyethylene [ 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Quinoline on the Phospholipid Profile of Curvularia lunata and Its Microbial Detoxification

et al. 2022

View full text Add to dashboard Cite

Quinoline is an N-heterocyclic compound commonly found in wastewater, especially that derived from coal processing, chemical, and pharmaceutical industries. In the present study, the microscopic fungus Curvularia lunata IM 4417, which is known to degrade various xenobiotics, was used. The aim of the research was to study the elimination of quinoline and its influence on fungal phospholipids, which are considered to be excellent indicators of environmental monitoring. Quinoline biodegradation products and phospholipid contents were analyzed using gas chromatography–mass spectrometry and liquid chromatography–tandem mass spectrometry. C. lunata IM 4417 degraded quinoline, which led to the formation of conjugates of glucose with hydroxylated derivatives of the compound. Toxicity tests (Artoxkit M and Microtox assay) indicated that the elimination of lower concentrations of quinoline was efficient and led to a reduction in sample toxicity. The presence of quinoline also significantly affected the profile of fatty acids and phospholipids. The addition of quinoline to a culture of C. lunata IM 4417 caused an increase in the content of phosphatidylcholine (PC) and a decrease in the amount of phosphatidylethanolamine (PE), two major structural lipids. Additionally, decreases in the contents of phosphatidylinositol (PI) and phosphatidylserine (PS), which are responsible for tolerance to toxic substances, cell viability, and signal transduction, were noted. Thus, it can be concluded that the presence of quinoline modifies the membrane composition, and this change may be an important indicator of the presence of N-heterocyclic compounds or other toxins in the environment.

show abstract

Optimization of process parameters for efficient decolorization of Congo red by novel fungusCurvulariasp. using Box–Behnken design

Cited by 3 publications

References 28 publications

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Decolorisation of Azo Dye Congo Red (CR) by Termitomyces sp. Biomass

Effect of Quinoline on the Phospholipid Profile of Curvularia lunata and Its Microbial Detoxification

Contact Info

Product

Resources

About